Detection of reserve polymer synthesis genes in natural bacterial populations
Screening of indigenous bacteria for the presence of genetic information for the biosynthesis of storage polymers was performed in river water samples using PCR amplification combined with Southern blotting. Polyhydroxyalkanoate synthase genes and polyphosphate kinase genes were detected using templ...
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| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1997
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| Sumario: | Screening of indigenous bacteria for the presence of genetic information for the biosynthesis of storage polymers was performed in river water samples using PCR amplification combined with Southern blotting. Polyhydroxyalkanoate synthase genes and polyphosphate kinase genes were detected using template DNA from different laboratory and river isolated bacterial strains, and from total river water DNA. This approach could be a suitable alternative to the study of bacterial communities based on the analysis of genes instead of microorganisms. |
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| Bibliografía: | López, N.I., Floccari, M.E., Steinbüchel, A., García, A.F., Méndez, B.S., Effect of poly(3-hydroxybutyrate) content on the starvation-survival of bacteria in natural waters (1995) FEMS Microbiol. Ecol., 16, pp. 95-102 Schubert, P., Steinbüchel, A., Schlegel, H.G., Cloning of the Alcaligenes eutrophus genes or synthesis of poly-β-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli (1988) J. Bacteriol., 170, pp. 5837-5847 Peoples, O.P., Sinskey, A.J., Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC) (1989) J. Biol. Chem., 264, pp. 15298-15303 Matin, A., Veldhuis, C., Stegeman, V., Veenhuis, M., Selective advantage of a Spirillum sp. in a carbon-limited environment. Accumulation of poly-β-hydroxybutyric acid and its role in starvation (1979) J. Gen. Microbiol., 112, pp. 349-355 Malmcrona-Friberg, K., Tunlid, A., Marden, P., Kjelleberg, S., Odham, G., Chemical changes in cell envelope and poly-β-hydroxybutyrate during short term starvation of a marine bacterial isolate (1986) Arch. Microbiol., 144, pp. 340-345 Kornberg, A., Kornberg, S.R., Simms, E.S., Metaphosphate synthesis by an enzyme of Escherichia coli (1956) Biochim. Biophys. Acta, 20, pp. 215-227 Kornberg, S.R., Adenosine triphosphate synthesis from polyphosphate by an enzyme of Escherichia coli (1957) Biochim. Biophys. Acta, 26, pp. 294-300 Akiyama, M., Crooke, E., Kornberg, A., The polyphosphate kinase gene of Escherichia coli (1992) J. Biol. Chem., 267, pp. 22556-22561 Rao, N.N., Kornberg, A., Inorganic polyphosphate supports resistance and survival of stationary phase Escherichia coli (1996) J. Bacteriol., 178, pp. 1394-1400 Hobbie, J.E., Daley, R.J., Jasper, S., Use of Nuclepore filters for counting bacteria by fluorescence microscopy (1977) Appl. Environ. Microbiol., 33, pp. 1225-1228 Tao, Y.-P., Vary, P.S., Isolation and characterization of a sporulation lacZ fusion mutants of Bacillus megaterium (1991) J. Gen. Microbiol., 137, pp. 797-806 Yanisch-Perron, C., Vieira, J., Messing, J., Improved M13 cloning vectors and host strains: Nucleotide sequences of M13mp18 and pUC19 vectors (1985) Gene, 33, pp. 103-119 Ostle, A., Holt, J.G., Nile Blue a as a fluorescent stain for poly-β-hydroxybutyrate (1982) Appl. Environ. Microbiol., 44, pp. 238-241 Balows, A., (1991) Manual of Clinical Microbiology, , American Society for Microbiology, Washington, DC Bohall N.A., Jr., Vary, P.S., Transposition of Tn917 in Bacillus megaterium (1986) J. Bacteriol., 167, pp. 716-718 Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn., , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY Möller, A., Gustafsson, K., Jansson, J.K., Specific monitoring by PCR amplification and bioluminescence of firefly luciferase gene tagged bacteria added to environmental samples (1994) FEMS Microbiol. Ecol., 15, pp. 193-206 López, N.I., Pettinari, M.J., Méndez, B.S., Monitoring of the polyphosphate kinase gene added to natural water samples by PCR amplification (1996) Microbiología SEM, , in press Steinbüchel, A., Hustede, E., Liebergesell, M., Pieper, U., Timm, A., Valentin, H., Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria (1992) FEMS Microbiol. Rev., 103, pp. 217-230 Schembri, M.A., Bayly, R.C., Davies, J.K., Cloning and molecular analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: Evidence that gene is both plasmid and chromosomally located (1994) FEMS Microbiol. Lett., 118, pp. 145-152 Slater, S.C., Voige, W.H., Dennis, D.E., Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-β-hydroxybutyrate biosynthetic pathway (1988) J. Bacteriol., 170, pp. 4431-4436 Kornberg, A., Inorganic polyphosphate: Toward making a forgotten polymer unforgettable (1995) J. Bacteriol., 177, pp. 491-496 Kulaev, I.S., Vagabov, V.M., Polyphosphate metabolism in micro-organisms (1983) Adv. Microb. Physiol., 24, pp. 83-171 Ahn, K., Kornberg, A., Polyphosphate kinase from Escherichia coli (1990) J. Biol. Chem., 265, pp. 11734-11739 Sinigalliano, C.D., Kuhn, D.N., Jones, R.D., Amplification of the amoA gene from diverse species of ammonium-oxidizing bacteria and from an indigenous bacterial population from seawater (1995) Appl. Environ. Microbiol., 61, pp. 2702-2706 Goldenberger, D., Perschil, I., Ritzler, M., Altwegg, M., A simple 'universal' DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification (1995) PCR Methods Appl., 4, pp. 368-370 Comeau, Y., Hall, K.J., Hancock, R.E.W., Oldham, W.K., Biochemical model for enhanced biological phosphorus removal (1986) Water Res., 20, pp. 1511-1521 Pedrós-Alió, C., Mas-Castellà, J., Mas, J., Guerrero, R., Polyhydroxyalkanoate accumulation in planktonic and anaerobic environments (1990) Novel Biodegradable Microbial Polymers, pp. 263-274. , (Dawes, E.A., Ed.), Kluwer Academic, Dordrecht |
| ISSN: | 01686496 |
| DOI: | 10.1016/S0168-6496(96)00083-9 |