Expression and biochemical characterisation of recombinant AceA, a bacterial α-mannosyltransferase
Biosynthesis of repeat-unit polysaccharides and N-linked glycans proceeds by sequential transfer of sugars from the appropriate sugar donor to an activated lipid carrier. The transfer of each sugar is catalysed by a specific glycosyltransferase. The molecular basis of the specificity of sugar additi...
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1999
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| LEADER | 13722caa a22013217a 4500 | ||
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| 001 | PAPER-2591 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203201.0 | ||
| 008 | 190411s1999 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0032788714 | |
| 024 | 7 | |2 cas |a DNA, Recombinant; Mannosyltransferases, EC 2.4.1.- | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MGGEA | ||
| 100 | 1 | |a Geremia, R.A. | |
| 245 | 1 | 0 | |a Expression and biochemical characterisation of recombinant AceA, a bacterial α-mannosyltransferase |
| 260 | |c 1999 | ||
| 270 | 1 | 0 | |m Geremia, R.A.; Ctr. Rech. Macromol. Veg. (CNRS), Universite Joseph Fourier, BP 53X, F-38041 Grenoble Cedex 9, France; email: geremia@cermav.cnrs.fr |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Reuber, T.L., Walker, G.C., Biosynthesis of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti (1993) Cell, 74, pp. 269-280 | ||
| 504 | |a Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual (2nd Edn.), , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y | ||
| 504 | |a Schnaitman, C.A., Klena, J.D., Genetics of lipopolysaccharide biosynthesis in enteric bacteria (1993) Microbiol Rev, 57, pp. 655-682 | ||
| 504 | |a Semino, C.E., Dankert, M.A., In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations (1993) J Gen Microbiol, 139, pp. 2745-2756 | ||
| 504 | |a Studier, F.W., Rosenberg, A.H., Dunn, J.J., Dubendorff, J.W., Use of T7 RNA polymerase to direct expression of cloned genes (1990) Methods Enzymol, 185, pp. 60-89 | ||
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| 504 | |a Wakarchuk, W., Martin, A., Jennings, M.P., Moxon, E.R., Richards, J.C., Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in the expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis (1996) J Biol Chem, 271, pp. 19166-19173 | ||
| 504 | |a Wakarchuk, W.W., Cunningham, A., Watson, D.C., Young, N.M., Role of paired basic residues in the expression of active recombinant galactosyltransferases from the bacterial pathogen Neisseria meningitidis (1998) Protein Eng, 11, pp. 295-302 | ||
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| 504 | |a Wiggins, C.A., Munro, S., Activity of the yeast MNN1 alpha-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases (1998) Proc Natl Acad Sci USA, 95, pp. 7945-7950 | ||
| 520 | 3 | |a Biosynthesis of repeat-unit polysaccharides and N-linked glycans proceeds by sequential transfer of sugars from the appropriate sugar donor to an activated lipid carrier. The transfer of each sugar is catalysed by a specific glycosyltransferase. The molecular basis of the specificity of sugar addition is not yet well understood, mainly because of the difficulty of isolating these proteins. In this study, the aceA gene product expressed by Acetobacter xylinum, which is involved in the biosynthesis of the exopolysaccharide acetan, was overproduced in Escherichia coli and its function was characterised. The aceA ORF was subcloned into the expression vector pET29 in frame with the S·tag epitope. The recombinant protein was identified, and culture conditions were optimised for production of the soluble protein. The results of test reactions showed that AceA is able to transfer one α-mannose residue from GDP-mannose to cellobiose-P-P-lipid to produce α-mannose-cellobiose-P-P-lipid. AceA was not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity. |l eng | |
| 536 | |a Detalles de la financiación: UBA TY03, 01-00014-00269 | ||
| 536 | |a Detalles de la financiación: PIP4461 | ||
| 536 | |a Detalles de la financiación: Acknowledgements Jean Gagnon, Institut de Biologie Structurale (Grenoble, France) for protein sequencing, Valerie Chazalet and Catherine Gautier (CERMAV) for their expert technical assistance, and H. Driguez (CERMAV) for critical reading of the manuscript. The France-Argentina collaboration was funded by the ECOS program PA97B28. Work at the Buenos Aires laboratory was supported by grants from CONICET PIP4461, ANPCyT 01-00014-00269, and UBA TY03 (to LI). | ||
| 593 | |a Ctr. Rech. sur les Macromolec. Veg., CNRS, Université Joseph Fourier, BP 53X, F-38041 Grenoble Cedex 9, France | ||
| 593 | |a Inst. Invest. Bioquim. Fund. C., Fac. de Ciencias Exactas y Naturales, Consejo Nac. de Invest. Cie./Tec., Avda. Patricias Argentinas 451, 1045 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ACETAN |
| 690 | 1 | 0 | |a ACETOBACTER XYLINUM |
| 690 | 1 | 0 | |a BACTERIAL POLYSACCHARIDE |
| 690 | 1 | 0 | |a BIOSYNTHESIS |
| 690 | 1 | 0 | |a GLYCOSYLTRANSFERASE |
| 690 | 1 | 0 | |a BACTERIAL ENZYME |
| 690 | 1 | 0 | |a CELLOBIOSE |
| 690 | 1 | 0 | |a GUANOSINE DIPHOSPHATE MANNOSE |
| 690 | 1 | 0 | |a MANNOSE |
| 690 | 1 | 0 | |a MANNOSYLTRANSFERASE |
| 690 | 1 | 0 | |a RECOMBINANT ENZYME |
| 690 | 1 | 0 | |a ACETOBACTER |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a EXPRESSION VECTOR |
| 690 | 1 | 0 | |a GENE OVEREXPRESSION |
| 690 | 1 | 0 | |a MOLECULAR CLONING |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OPEN READING FRAME |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a STRUCTURE ACTIVITY RELATION |
| 690 | 1 | 0 | |a AMINO ACID SEQUENCE |
| 690 | 1 | 0 | |a BASE SEQUENCE |
| 690 | 1 | 0 | |a CARBOHYDRATE SEQUENCE |
| 690 | 1 | 0 | |a CLONING, MOLECULAR |
| 690 | 1 | 0 | |a DNA, RECOMBINANT |
| 690 | 1 | 0 | |a MANNOSYLTRANSFERASES |
| 690 | 1 | 0 | |a MUTAGENESIS, SITE-DIRECTED |
| 690 | 1 | 0 | |a OPEN READING FRAMES |
| 690 | 1 | 0 | |a SUBSTRATE SPECIFICITY |
| 690 | 1 | 0 | |a ACETOBACTER |
| 690 | 1 | 0 | |a BACTERIA (MICROORGANISMS) |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a GLUCONACETOBACTER XYLINUS |
| 700 | 1 | |a Roux, M. | |
| 700 | 1 | |a Ferreiro, D.U. | |
| 700 | 1 | |a Dauphin-Dubois, R. | |
| 700 | 1 | |a Lellouch, A.C. | |
| 700 | 1 | |a Ielpi, L. | |
| 773 | 0 | |d 1999 |g v. 261 |h pp. 933-940 |k n. 6 |p Mol. Gen. Genet. |x 00268925 |w (AR-BaUEN)CENRE-3025 |t Molecular and General Genetics | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00268925_v261_n6_p933_Geremia |y Handle |
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