Kinetic recognition of the retinoblastoma tumor suppressor by a specific protein target
The retinoblastoma tumor suppressor (Rb) plays a key role in cell cycle control and is linked to various types of human cancer. Rb binds to the LxCxE motif, present in a number of cellular and viral proteins such as AdE1A, SV40 large T-antigen and human papillomavirus (HPV) E7, all instrumental in r...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2011
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 25582caa a22017657a 4500 | ||
|---|---|---|---|
| 001 | PAPER-23617 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205519.0 | ||
| 008 | 190411s2011 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-80052021703 | |
| 024 | 7 | |2 cas |a Papillomavirus E7 Proteins; Retinoblastoma Protein; oncogene protein E7, Human papillomavirus type 16 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JMOBA | ||
| 100 | 1 | |a Chemes, L.B. | |
| 245 | 1 | 0 | |a Kinetic recognition of the retinoblastoma tumor suppressor by a specific protein target |
| 260 | |c 2011 | ||
| 270 | 1 | 0 | |m De Prat-Gay, G.; Protein Structure-Function and Engineering Laboratory, Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, 1405 Buenos Aires, Argentina; email: gpg@leloir.org.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Chinnam, M., Goodrich, D.W., RB1, development, and cancer (2011) Curr. Top. Dev. Biol., 94, pp. 129-169 | ||
| 504 | |a Longworth, M.S., Dyson, N.J., PRb, a local chromatin organizer with global possibilities (2010) Chromosoma, 119, pp. 1-11 | ||
| 504 | |a Van Den Heuvel, S., Dyson, N.J., Conserved functions of the pRB and E2F families (2008) Nat. Rev., Mol. Cell Biol., 9, pp. 713-724 | ||
| 504 | |a Burkhart, D.L., Sage, J., Cellular mechanisms of tumour suppression by the retinoblastoma gene (2008) Nat. Rev., Cancer, 8, pp. 671-682 | ||
| 504 | |a Howley, P.M., Livingston, D.M., Small DNA tumor viruses: Large contributors to biomedical sciences (2009) Virology, 384, pp. 256-259 | ||
| 504 | |a Morris, E.J., Dyson, N.J., Retinoblastoma protein partners (2001) Advances in Cancer Research, 82, pp. 1-54. , DOI 10.1016/S0065-230X(01)82001-7 | ||
| 504 | |a Degregori, J., The Rb network (2004) J. Cell Sci., 117, pp. 3411-3413 | ||
| 504 | |a Isaac, C.E., Francis, S.M., Martens, A.L., Julian, L.M., Seifried, L.A., Erdmann, N., The retinoblastoma protein regulates pericentric heterochromatin (2006) Mol. Cell. Biol., 26, pp. 3659-3671 | ||
| 504 | |a Xiao, B., Spencer, J., Clements, A., Ali-Khan, N., Mittnacht, S., Broceno, C., Burghammer, M., Gamblin, S.J., Crystal structure of the retinoblastoma tumor suppressor protein bound to E2F and the molecular basis of its regulation (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (5), pp. 2363-2368. , DOI 10.1073/pnas.0436813100 | ||
| 504 | |a Lee, C., Chang, J.H., Lee, H.S., Cho, Y., Structural basis for the recognition of the E2F transactivation domain by the retinoblastoma tumor suppressor (2002) Genes and Development, 16 (24), pp. 3199-3212. , DOI 10.1101/gad.1046102 | ||
| 504 | |a Singh, M., Krajewski, M., Mikolajka, A., Holak, T.A., Molecular determinants for the complex formation between the retinoblastoma protein and LXCXE sequences (2005) Journal of Biological Chemistry, 280 (45), pp. 37868-37876. , DOI 10.1074/jbc.M504877200 | ||
| 504 | |a Dick, F.A., Structure-function analysis of the retinoblastoma tumor suppressor protein-is the whole a sum of its parts? (2007) Cell Div., 2, p. 26 | ||
| 504 | |a Burke, J.R., Deshong, A.J., Pelton, J.G., Rubin, S.M., Phosphorylation-induced conformational changes in the retinoblastoma protein inhibit E2F transactivation domain binding (2010) J. Biol. Chem., 285, pp. 16286-16293 | ||
| 504 | |a Rubin, S.M., Gall, A.-L., Zheng, N., Pavletich, N.P., Structure of the Rb C-terminal domain bound to E2F1-DP1: A mechanism for phosphorylation-induced E2F release (2005) Cell, 123 (6), pp. 1093-1106. , DOI 10.1016/j.cell.2005.09.044, PII S0092867405012316 | ||
| 504 | |a De Souza, R.F., Iyer, L.M., Aravind, L., Diversity and evolution of chromatin proteins encoded by DNA viruses (2009) Biochim. Biophys. Acta, 1799, pp. 302-318 | ||
| 504 | |a Kehn, K., De La Fuente, C., Strouss, K., Berro, R., Jiang, H., Brady, J., Mahieux, R., Kashanchi, F., The HTLV-I Tax oncoprotein targets the retinoblastoma protein for proteasomal degradation (2005) Oncogene, 24 (4), pp. 525-540. , DOI 10.1038/sj.onc.1208105 | ||
| 504 | |a Munakata, T., Nakamura, M., Liang, Y., Li, K., Lemon, S.M., Down-regulation of the retinoblastoma tumor suppressor by the hepatitis C virus NS5B RNA-dependent RNA polymerase (2005) Proceedings of the National Academy of Sciences of the United States of America, 102 (50), pp. 18159-18164. , DOI 10.1073/pnas.0505605102 | ||
| 504 | |a Forng, R.-Y., Atreya, C.D., Mutations in the retinoblastoma protein-binding LXCXE motif of rubella virus putative replicase affect virus replication (1999) Journal of General Virology, 80 (2), pp. 327-332 | ||
| 504 | |a Davey, N.E., Trave, G., Gibson, T.J., How viruses hijack cell regulation (2011) Trends Biochem. Sci., 36, pp. 159-169 | ||
| 504 | |a Lee, J.-O., Russo, A.A., Pavletich, N.P., Structure of the retinoblastoma tumour-suppressor pocket domain bound to a peptide from HPV E7 (1998) Nature, 391 (6670), pp. 859-865. , DOI 10.1038/36038 | ||
| 504 | |a Kim, H.-Y., Ahn, B.-Y., Cho, Y., Structural basis for the inactivation of retinoblastoma tumor suppressor by SV40 large T antigen (2001) EMBO Journal, 20 (1-2), pp. 295-304. , DOI 10.1093/emboj/20.1.295 | ||
| 504 | |a Ferreon, J.C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E., Structural basis for subversion of cellular control mechanisms by the adenoviral E1A oncoprotein (2009) Proc. Natl Acad. Sci. USA, 106, pp. 13260-13265 | ||
| 504 | |a Liu, X., Marmorstein, R., Structure of the retinoblastoma protein bound to adenovirus E1A reveals the molecular basis for viral oncoprotein inactivation of a tumor suppressor (2007) Genes and Development, 21 (21), pp. 2711-2716. , http://www.genesdev.org/cgi/reprint/21/21/2711, DOI 10.1101/gad.1590607 | ||
| 504 | |a Dyson, N., Guida, P., McCall, C., Harlow, E., Adenovirus E1A makes two distinct contacts with the retinoblastoma protein (1992) J. Virol., 66, pp. 4606-4611 | ||
| 504 | |a Patrick, D.R., Oliff, A., Heimbrook, D.C., Identification of a novel retinoblastoma gene product binding site on human papillomavirus type 16 E7 protein (1994) Journal of Biological Chemistry, 269 (9), pp. 6842-6850 | ||
| 504 | |a Block, C., Janknecht, R., Herrmann, C., Nassar, N., Wittinghofer, A., Quantitative structure-activity analysis correlating Ras/Raf interaction in vitro to Raf activation in vivo (1996) Nature Structural Biology, 3 (3), pp. 244-251. , DOI 10.1038/nsb0396-244 | ||
| 504 | |a Kiel, C., Serrano, L., Cell type-specific importance of ras-c-raf complex association rate constants for MAPK signaling (2009) Sci. Signal., 2, p. 38 | ||
| 504 | |a Schreiber, G., Kinetic studies of protein-protein interactions (2002) Current Opinion in Structural Biology, 12 (1), pp. 41-47. , DOI 10.1016/S0959-440X(02)00287-7 | ||
| 504 | |a Phelps, W.C., Yee, C.L., Munger, K., Howley, P.M., The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1A (1988) Cell, 53, pp. 539-547 | ||
| 504 | |a Banerjee, N.S., Genovese, N.J., Noya, F., Chien, W.-M., Broker, T.R., Chow, L.T., Conditionally activated E7 proteins of high-risk and low-risk human papillomaviruses induce S phase in postmitotic, differentiated human keratinocytes (2006) Journal of Virology, 80 (13), pp. 6517-6524. , DOI 10.1128/JVI.02499-05 | ||
| 504 | |a Garcia-Alai, M.M., Alonso, L.G., De Prat-Gay, G., The N-terminal module of HPV16 E7 is an intrinsically disordered domain that confers conformational and recognition plasticity to the oncoprotein (2007) Biochemistry, 46 (37), pp. 10405-10412. , DOI 10.1021/bi7007917 | ||
| 504 | |a Uversky, V.N., Roman, A., Oldfield, C.J., Dunker, A.K., Protein intrinsic disorder and human papillomaviruses: Increased amount of disorder in E6 and E7 oncoproteins from high risk HPVs (2006) Journal of Proteome Research, 5 (8), pp. 1829-1842. , DOI 10.1021/pr0602388 | ||
| 504 | |a Alonso, L.G., Garcia-Alai, M.M., Nadra, A.D., Lapena, A.N., Almeida, F.L., Gualfetti, P., Prat-Gay, G.D., High-risk (HPV16) human papillomavirus E7 oncoprotein is highly stable and extended, with conformational transitions that could explain its multiple cellular binding partners (2002) Biochemistry, 41, pp. 10510-10518 | ||
| 504 | |a Liu, X., Clements, A., Zhao, K., Marmorstein, R., Structure of the human Papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor (2006) Journal of Biological Chemistry, 281 (1), pp. 578-586. , http://www.jbc.org/cgi/reprint/281/1/578.pdf, DOI 10.1074/jbc.M508455200 | ||
| 504 | |a Ohlenschlager, O., Seiboth, T., Zengerling, H., Briese, L., Marchanka, A., Ramachandran, R., Baum, M., Gorlach, M., Solution structure of the partially folded high-risk human papilloma virus 45 oncoprotein E7 (2006) Oncogene, 25 (44), pp. 5953-5959. , DOI 10.1038/sj.onc.1209584, PII 1209584 | ||
| 504 | |a Chemes, L.B., Sanchez, I.E., Smal, C., De Prat-Gay, G., Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein. Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 (2010) FEBS J., 277, pp. 973-988 | ||
| 504 | |a Rechsteiner, M., Rogers, S.W., PEST sequences and regulation by proteolysis (1996) Trends in Biochemical Sciences, 21 (7), pp. 267-271. , DOI 10.1016/0968-0004(96)10031-1 | ||
| 504 | |a Dick, F.A., Dyson, N.J., Three regions of the pRB pocket domain affect its inactivation by human papillomavirus E7 proteins (2002) Journal of Virology, 76 (12), pp. 6224-6234. , DOI 10.1128/JVI.76.12.6224-6234.2002 | ||
| 504 | |a Ho Man Chan, Smith, L., La Thangue, N.B., Role of LXCXE motif-dependent interactions in the activity of the retinoblastoma protein (2001) Oncogene, 20 (43), pp. 6152-6163. , DOI 10.1038/sj.onc.1204793 | ||
| 504 | |a Munger, K., Yee, C.L., Phelps, W.C., Pietenpol, J.A., Moses, H.L., Howley, P.M., Biochemical and biological differences between E7 oncoproteins of the high- and low-risk human papillomavirus types are determined by amino-terminal sequences (1991) J. Virol., 65, pp. 3943-3948 | ||
| 504 | |a Huang, P.S., Patrick, D.R., Edwards, G., Goodhart, P.J., Huber, H.E., Miles, L., Garsky, V.M., Heimbrook, D.C., Protein domains governing interactions between E2F, the retinoblastoma gene product, and human papillomavirus type 16 E7 protein (1993) Molecular and Cellular Biology, 13 (2), pp. 953-960 | ||
| 504 | |a Heck, D.V., Yee, C.L., Howley, P.M., Munger, K., Efficiency of binding the retinoblastoma protein correlates with the transforming capacity of the E7 oncoproteins of the human papillomaviruses (1992) Proc. Natl Acad. Sci. USA, 89, pp. 4442-4446 | ||
| 504 | |a Xue, B., Williams, R.W., Oldfield, C.J., Goh, G.K., Dunker, A.K., Uversky, V.N., Viral disorder or disordered viruses: Do viral proteins possess unique features? (2010) Protein Pept. Lett., 17, pp. 932-951 | ||
| 504 | |a Chemes, L.B., Sánchez, I.E., Alonso, L.G., De Prat-Gay, G., Intrinsic Disorder in the human papillomavirus E7 protein (2011) Flexible Viruses: Structural Disorder Within Viral Proteins, , (Longhi, S., Uversky, V.N., ed.), pp. in press. John Wiley and Sons | ||
| 504 | |a Shoemaker, B.A., Portman, J.J., Wolynes, P.G., Speeding molecular recognition by using the folding funnel: The fly-casting mechanism (2000) Proceedings of the National Academy of Sciences of the United States of America, 97 (16), pp. 8868-8873. , DOI 10.1073/pnas.160259697 | ||
| 504 | |a Helt, A.-M., Galloway, D.A., Mechanisms by which DNA tumor virus oncoproteins target the Rb family of pocket proteins (2003) Carcinogenesis, 24 (2), pp. 159-169. , DOI 10.1093/carcin/24.2.159 | ||
| 504 | |a Magnaghi-Jaulin, L., Groisman, R., Naguibneva, I., Robin, P., Lorain, S., Le Villain, J.P., Troalen, F., Harel-Bellan, A., Retinoblastoma protein represses transcription by recruiting a histone deacetylase (1998) Nature, 391 (6667), pp. 601-605. , DOI 10.1038/35410 | ||
| 504 | |a Stokes, P.H., Thompson, L.S., Marianayagam, N.J., Matthews, J.M., Dimerization of CtIP may stabilize in vivo interactions with the Retinoblastoma-pocket domain (2007) Biochemical and Biophysical Research Communications, 354 (1), pp. 197-202. , DOI 10.1016/j.bbrc.2006.12.178, PII S0006291X06028580 | ||
| 504 | |a Dick, F.A., Sailhamer, E., Dyson, N.J., Mutagenesis of the pRB pocket reveals that cell cycle arrest functions are separable from binding to viral oncoproteins (2000) Molecular and Cellular Biology, 20 (10), pp. 3715-3727. , DOI 10.1128/MCB.20.10.3715-3727.2000 | ||
| 504 | |a MacLellan, W.R., Xiao, G., Abdellatif, M., Schneider, M.D., A novel Rb- and p300-binding protein inhibits transactivation by MyoD (2000) Molecular and Cellular Biology, 20 (23), pp. 8903-8915. , DOI 10.1128/MCB.20.23.8903-8915.2000 | ||
| 504 | |a Miyake, S., Sellers, W.R., Safran, M., Li, X., Zhao, W., Grossman, S.R., Gan, J., Kaelin W.G., Jr., Cells degrade a novel inhibitor of differentiation with e1a-like properties upon exiting the cell cycle (2000) Molecular and Cellular Biology, 20 (23), pp. 8889-8902. , DOI 10.1128/MCB.20.23.8889-8902.2000 | ||
| 504 | |a Joerger, A.C., Fersht, A.R., Structural biology of the tumor suppressor p53 (2008) Annu. Rev. Biochem., 77, pp. 557-582 | ||
| 504 | |a Clements, A., Johnston, K., Mazzarelli, J.M., Ricciardi, R.P., Marmorstein, R., Oligomerization properties of the viral oncoproteins adenovirus E1A and human papillomavirus E7 and their complexes with the retinoblastoma protein (2000) Biochemistry, 39 (51), pp. 16033-16045. , DOI 10.1021/bi002111g | ||
| 504 | |a Sheinerman, F.B., Norel, R., Honig, B., Electrostatic aspects of protein-protein interactions (2000) Current Opinion in Structural Biology, 10 (2), pp. 153-159. , DOI 10.1016/S0959-440X(00)00065-8 | ||
| 504 | |a Vindigni, A., White, C.E., Komives, E.A., Di Cera, E., Energetics of thrombin-thrombomodulin interaction (1997) Biochemistry, 36 (22), pp. 6674-6681. , DOI 10.1021/bi962766a | ||
| 504 | |a Grucza, R.A., Bradshaw, J.M., Mitaxov, V., Waksman, G., Role of electrostatic interactions in SH2 domain recognition: Salt-dependence of tyrosyl-phosphorylated peptide binding to the tandem SH2 domain of the Syk kinase and the single SH2 domain of the Src kinase (2000) Biochemistry, 39 (33), pp. 10072-10081. , DOI 10.1021/bi000891n | ||
| 504 | |a Fersht, A.R., Matouschek, A., Serrano, L., The folding of an enzyme. I. Theory of protein engineering analysis of stability and pathway of protein folding (1992) J. Mol. Biol., 224, pp. 771-782 | ||
| 504 | |a Chien, W.-M., Parker, J.N., Schmidt-Grimminger, D.-C., Broker, T.R., Chow, L.T., Casein kinase II phosphorylation of the human papillomavirus-18 E7 protein is critical for promoting S-phase entry (2000) Cell Growth and Differentiation, 11 (8), pp. 425-435 | ||
| 504 | |a Phelps, W.C., Munger, K., Yee, C.L., Barnes, J.A., Howley, P.M., Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein (1992) J. Virol., 66, pp. 2418-2427 | ||
| 504 | |a Genovese, N.J., Banerjee, N.S., Broker, T.R., Chow, L.T., Casein kinase II motif-dependent phosphorylation of human papillomavirus E7 protein promotes p130 degradation and S-phase induction in differentiated human keratinocytes (2008) Journal of Virology, 82 (10), pp. 4862-4873. , DOI 10.1128/JVI.01202-07 | ||
| 504 | |a Munger, K., Werness, B.A., Dyson, N., Phelps, W.C., Harlow, E., Howley, P.M., Complex formation of c-myc papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product (1989) EMBO Journal, 8 (13), pp. 4099-4105 | ||
| 504 | |a Hassler, M., Singh, S., Yue, W.W., Luczynski, M., Lakbir, R., Sanchez-Sanchez, F., Bader, T., Mittnacht, S., Crystal Structure of the Retinoblastoma Protein N Domain Provides Insight into Tumor Suppression, Ligand Interaction, and Holoprotein Architecture (2007) Molecular Cell, 28 (3), pp. 371-385. , DOI 10.1016/j.molcel.2007.08.023, PII S109727650700593X | ||
| 504 | |a Schreiber, G., Fersht, A.R., Interaction of barnase with its polypeptide inhibitor barstar studied by protein engineering (1993) Biochemistry, 32 (19), pp. 5145-5150. , DOI 10.1021/bi00070a025 | ||
| 504 | |a Myles, T., Le Bonniec, B.F., Betz, A., Stone, S.R., Electrostatic steering and ionic tethering in the formation of thrombin-hirudin complexes: The role of the thrombin anion-binding exosite-I (2001) Biochemistry, 40 (16), pp. 4972-4979. , DOI 10.1021/bi0023549 | ||
| 504 | |a Kiel, C., Selzer, T., Shaul, Y., Schreiber, G., Herrmann, C., Electrostatically optimized Ras-binding Ral guanine dissociation stimulator mutants increase the rate of association by stabilizing the encounter complex (2004) Proceedings of the National Academy of Sciences of the United States of America, 101 (25), pp. 9223-9228. , DOI 10.1073/pnas.0401160101 | ||
| 504 | |a Gianni, S., Engstrom, A., Larsson, M., Calosci, N., Malatesta, F., Eklund, L., Ngang, C.C., Jemth, P., The kinetics of PDZ domain-ligand interactions and implications for the binding mechanism (2005) Journal of Biological Chemistry, 280 (41), pp. 34805-34812. , DOI 10.1074/jbc.M506017200 | ||
| 504 | |a Stewart, R.C., Van Bruggen, R., Association and Dissociation Kinetics for CheY Interacting with the P2 Domain of CheA (2004) Journal of Molecular Biology, 336 (1), pp. 287-301. , DOI 10.1016/j.jmb.2003.11.059 | ||
| 504 | |a Zhang, Y., Wavreille, A.S., Kunys, A.R., Pei, D., The SH2 domains of inositol polyphosphate 5-phosphatases SHIP1 and SHIP2 have similar ligand specificity but different binding kinetics (2009) Biochemistry, 48, pp. 11075-11083 | ||
| 504 | |a Radic, Z., Kirchhoff, P.D., Quinn, D.M., McCammon, J.A., Taylor, P., Electrostatic influence on the kinetics of ligand binding to acetylcholinesterase: Distinctions between active center ligands and fasciculin (1997) Journal of Biological Chemistry, 272 (37), pp. 23265-23277. , DOI 10.1074/jbc.272.37.23265 | ||
| 504 | |a Rihs, H.-P., Jans, D.A., Fan, H., Peters, R., The rate of nuclear cytoplasmic protein transport is determined by the casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigen (1991) EMBO Journal, 10 (3), pp. 633-639 | ||
| 504 | |a Whalen, S.G., Marcellus, R.C., Barbeau, D., Branton, P.E., Importance of the Ser-132 phosphorylation site in cell transformation and apoptosis induced by the adenovirus type 5 E1A protein (1996) Journal of Virology, 70 (8), pp. 5373-5383 | ||
| 504 | |a Grove, T.Z., Cortajarena, A.L., Regan, L., Ligand binding by repeat proteins: Natural and designed (2008) Curr. Opin. Struct. Biol., 18, pp. 507-515 | ||
| 504 | |a Remenyi, A., Good, M.C., Lim, W.A., Docking interactions in protein kinase and phosphatase networks (2006) Current Opinion in Structural Biology, 16 (6), pp. 676-685. , DOI 10.1016/j.sbi.2006.10.008, PII S0959440X06001801, Catalysis and Regulation / Proteins | ||
| 504 | |a Mittag, T., Kay, L.E., Forman-Kay, J.D., Protein dynamics and conformational disorder in molecular recognition (2009) J. Mol. Recognit., 23, pp. 105-116 | ||
| 504 | |a Uversky, V.N., Multitude of binding modes attainable by intrinsically disordered proteins: A portrait gallery of disorder-based complexes (2010) Chem. Soc. Rev., 40, pp. 1623-1634 | ||
| 504 | |a Ferreiro, D.U., Hegler, J.A., Komives, E.A., Wolynes, P.G., On the role of frustration in the energy landscapes of allosteric proteins (2011) Proc. Natl Acad. Sci. USA, 108, pp. 3499-3503 | ||
| 504 | |a Balog, E.R.M., Burke, J.R., Hura, G.L., Rubin, S.M., Crystal structure of the unliganded retinoblastoma protein pocket domain (2011) Proteins: Struct., Funct., Bioinf., 79, pp. 2010-2014 | ||
| 504 | |a Sugase, K., Dyson, H.J., Wright, P.E., Mechanism of coupled folding and binding of an intrinsically disordered protein (2007) Nature, 447 (7147), pp. 1021-1025. , DOI 10.1038/nature05858, PII NATURE05858 | ||
| 504 | |a Turjanski, A.G., Gutkind, J.S., Best, R.B., Hummer, G., Binding-induced folding of a natively unstructured transcription factor (2008) PLoS Comput. Biol., 4, p. 1000060 | ||
| 504 | |a Whitford, P.C., Onuchic, J.N., Wolynes, P.G., Energy landscape along an enzymatic reaction trajectory: Hinges or cracks? (2008) HFSP J., 2, pp. 61-64 | ||
| 504 | |a Pace, C.N., Vajdos, F., Fee, L., Grimsley, G., Gray, T., How to measure and predict the molar absorption coefficient of a protein (1995) Protein Sci., 4, pp. 2411-2423 | ||
| 504 | |a Hermanson, G.T., (1996) Bioconjugate Techniques, , Academic Press San Diego, CA | ||
| 504 | |a Malatesta, F., The study of bimolecular reactions under non-pseudo-first order conditions (2005) Biophysical Chemistry, 116 (3), pp. 251-256. , DOI 10.1016/j.bpc.2005.04.006, PII S0301462205000864 | ||
| 504 | |a Chi, C.N., Bach, A., Engstrom, A., Wang, H., Stromgaard, K., Gianni, S., Jemth, P., A sequential binding mechanism in a PDZ domain (2009) Biochemistry, 48, pp. 7089-7097 | ||
| 520 | 3 | |a The retinoblastoma tumor suppressor (Rb) plays a key role in cell cycle control and is linked to various types of human cancer. Rb binds to the LxCxE motif, present in a number of cellular and viral proteins such as AdE1A, SV40 large T-antigen and human papillomavirus (HPV) E7, all instrumental in revealing fundamental mechanisms of tumor suppression, cell cycle control and gene expression. A detailed kinetic study of RbAB binding to the HPV E7 oncoprotein shows that an LxCxE-containing E7 fragment binds through a fast two-state reaction strongly favored by electrostatic interactions. Conversely, full-length E7 binds through a multistep process involving a pre-equilibrium between E7 conformers, a fast electrostatically driven association step guided by the LxCxE motif and a slow conformational rearrangement. This kinetic complexity arises from the conformational plasticity and intrinsically disordered nature of E7 and from multiple interaction surfaces present in both proteins. Affinity differences between E7N domains from high- and low-risk types are explained by their dissociation rates. In fact, since Rb is at the center of a large protein interaction network, fast and tight recognition provides an advantage for disruption by the viral proteins, where the balance of physiological and pathological interactions is dictated by kinetic ligand competition. The localization of the LxCxE motif within an intrinsically disordered domain provides the fast, diffusion-controlled interaction that allows viral proteins to outcompete physiological targets. We describe the interaction mechanism of Rb with a protein ligand, at the same time an LxCxE-containing model target, and a paradigmatic intrinsically disordered viral oncoprotein. © 2011 Elsevier Ltd. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Fundación YPF | ||
| 536 | |a Detalles de la financiación: L.B.C. currently holds a postdoctoral fellowship from Consejo Nacional de Investigaciones Científicas y Técnicas and was supported throughout the work by a José A. Estenssoro predoctoral fellowship from Fundación YPF . I.E.S. and G.d.P.-G. are career investigators from Consejo Nacional de Investigaciones Científicas y Técnicas. We thank M. Trevisan for helpful advice on quantitative modeling of the E7:Rb interaction. Appendix A | ||
| 593 | |a Protein Structure-Function and Engineering Laboratory, Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, 1405 Buenos Aires, Argentina | ||
| 593 | |a Protein Physiology Laboratory, Departamento de Quimica Biologica, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a INTRINSICALLY DISORDERED PROTEINS |
| 690 | 1 | 0 | |a LXCXE MOTIF |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a RETINOBLASTOMA PROTEIN |
| 690 | 1 | 0 | |a VIRAL ONCOPROTEIN |
| 690 | 1 | 0 | |a PROTEIN E7 |
| 690 | 1 | 0 | |a RETINOBLASTOMA BINDING PROTEIN |
| 690 | 1 | 0 | |a VIRUS PROTEIN |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CANCER INHIBITION |
| 690 | 1 | 0 | |a CELL CYCLE REGULATION |
| 690 | 1 | 0 | |a GENE EXPRESSION |
| 690 | 1 | 0 | |a HUMAN PAPILLOMAVIRUS TYPE 16 |
| 690 | 1 | 0 | |a MOLECULAR RECOGNITION |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN CONFORMATION |
| 690 | 1 | 0 | |a PROTEIN DOMAIN |
| 690 | 1 | 0 | |a PROTEIN MOTIF |
| 690 | 1 | 0 | |a PROTEIN PROTEIN INTERACTION |
| 690 | 1 | 0 | |a PROTEIN TARGETING |
| 690 | 1 | 0 | |a SIMIAN VIRUS 40 |
| 690 | 1 | 0 | |a TUMOR SUPPRESSOR GENE |
| 690 | 1 | 0 | |a AMINO ACID SEQUENCE |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MODELS, MOLECULAR |
| 690 | 1 | 0 | |a MOLECULAR SEQUENCE DATA |
| 690 | 1 | 0 | |a PAPILLOMAVIRUS E7 PROTEINS |
| 690 | 1 | 0 | |a PROTEIN BINDING |
| 690 | 1 | 0 | |a RETINOBLASTOMA PROTEIN |
| 690 | 1 | 0 | |a SEQUENCE HOMOLOGY, AMINO ACID |
| 690 | 1 | 0 | |a THERMODYNAMICS |
| 690 | 1 | 0 | |a HUMAN PAPILLOMAVIRUS |
| 700 | 1 | |a Sánchez, I.E. | |
| 700 | 1 | |a De Prat-Gay, G. | |
| 773 | 0 | |d 2011 |g v. 412 |h pp. 267-284 |k n. 2 |p J. Mol. Biol. |x 00222836 |w (AR-BaUEN)CENRE-1757 |t Journal of Molecular Biology | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052021703&doi=10.1016%2fj.jmb.2011.07.015&partnerID=40&md5=85c19821f32d4427ea6e6ae3a51596fe |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.jmb.2011.07.015 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00222836_v412_n2_p267_Chemes |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222836_v412_n2_p267_Chemes |y Registro en la Biblioteca Digital |
| 961 | |a paper_00222836_v412_n2_p267_Chemes |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 84570 | ||