Phosphotransbutyrylase expression in Bacillus megaterium

Mostrar otras versiones (1)

The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponentia...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Vazquez, G.J
Otros Autores: Pettinari, M.J, Méndez, B.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2001
Acceso en línea:Registro en Scopus
DOI
Handle
Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
LEADER 07564caa a22009257a 4500
001 PAPER-2047
003 AR-BaUEN
005 20230518203122.0
008 190411s2001 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-0035078620 
024 7 |2 Molecular Sequence Numbers  |a EMBL: AJ278958; 
024 7 |2 cas  |a Amino Acids, Branched-Chain; Culture Media; DNA-Binding Proteins; DNA-Directed RNA Polymerases, EC 2.7.7.6; Phosphate Acetyltransferase, EC 2.3.1.8; RNA Polymerase Sigma 54, EC 2.7.7.6; Sigma Factor 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a CUMID 
100 1 |a Vazquez, G.J. 
245 1 0 |a Phosphotransbutyrylase expression in Bacillus megaterium 
260 |c 2001 
270 1 0 |m Méndez, B.S.; Departamento de Quimica Biologica, Ciudad Universitaria Pabellon 2, Fac. de Ciencias Exactas y Naturales, 1428 Buenos Aires, Argentina; email: bea@qb.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Bullock, W., Fernández, J., Short, J., XL1-Blue: A high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection (1987) Biotechniques, 5, pp. 376-379 
504 |a Cary, J., Petersen, D., Papoutaskis, E., Bennett, G., Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli (1988) J Bacteriol, 170, pp. 4613-4618 
504 |a Debarbouille, M., Rozzen, G., Maryvonne, A., Rapoport, G., Role of BkdR, a transcriptional activator of the SigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis (1999) J Bacteriol, 181, pp. 2059-2066 
504 |a English, J., Vary, P., Isolation of recombination deficient and UV-sensitive mutants of Bacillus megaterium (1986) J Bacteriol, 165, pp. 155-160 
504 |a Feigenbaum, J., Schultz, H., Thiolases of Escherichia coli: Purification and chain length specificities (1975) J Bacteriol, 122, pp. 407-411 
504 |a Grundy, F., Waters, D., Tsetska, H., Henkin, T., Identification of genes involved in utilization of acetate and acetoin in B. subtillis (1993) Mol Microbiol, 10, pp. 259-271 
504 |a Hull, R., Gill, R., Hsu, P., Minshew, P., Falkow, S., Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from urinary track infection Escherichia coli isolate (1981) Infect Immun, 33, pp. 933-938 
504 |a Jenkins, L., Nunn, W., Regulation of the ato operon by the atoC gene in Escherichia coli (1987) J Bacteriol, 169, pp. 2096-2102 
504 |a Jones, D., Woods, D., Acetone-butanol fermentation revisited (1986) Microbiol Rev, 50, pp. 484-524 
504 |a Kunst, F., Ogasawara, N., Moszer, I., Albertini, A., Alloni, G., The complete genome sequence of the Gram-positive bacterium Bacillus subtilis (1997) Nature, 390, pp. 249-256 
504 |a Lowry, O., Rosebrough, N., Farr, L., Randall, R., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 
504 |a Massey, K., Schmidt, J., Brody, S., Branched-chain amino acid catabolism in bacteria (1976) Bacteriol Rev, 40, pp. 42-54 
504 |a Pettinari, M.J., Vazquez, G.J., Kruger, N., Vary, P., Steinbüchel, A., Méndez, B.S., Trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoates production (1998) Appl Microbiol Biotechnol, 49, pp. 737-742 
504 |a Rado, T., Hoch, J., Phosphotransacetylase from Bacillus subtilis: Purification and physiological studies (1973) Biochim Biophys Acta, 321, pp. 114-125 
504 |a Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular cloning: a laboratory manual, 2nd edn., , Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, Press 
504 |a Shingler, V., Signal sensing by σ54 dependent regulators: Derepression as a control mechanism (1996) Mol Microbiol, 19, pp. 409-416 
504 |a Shulgovskaya, E., Pozmogova, E., Rabotnova, I., Effect of protein synthesis inhibitor chloramphenicol: Basic growth parameters of continuous and batch cultures of Bacillus megaterium (1981) Mikrobiologiya, 49, pp. 893-901 
504 |a Trieu-Cuot, P., Carlier, C., Poyart-Salmeron, C., Courvalin, P., Shuttle vectors containing a multiple cloning site and a lacZ gene for conjugal transfer of DNA from Escherichia coli to gram positive bacteria (1991) Gene, 102, pp. 99-104 
504 |a Vary, P., Tao, Y.P., Development of genetic methods in Bacillus megaterium (1988), 2, pp. 403-407. , Ganesan AT, Hoch JA (eds) Genetics and biotechnology of bacilli. New York: Academic Press; Vazquez, G.J., Pettinari, M.J., Méndez, B.S., (1997), EMBL nucleotide accession number: Y14588; Walter, K., Nair, R., Cary, J., Bennett, G., Papoutsakis, E., Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824 (1993) Gene, 134, pp. 107-111 
520 3 |a The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponential growth phase. The branched amino acids isoleucine and valine activated Ptb expression. PtbBm was capable of using butyryl-CoA and 2-methyl-propionyl CoA as substrates. ActBm, a σ54 regulator from B. megaterium whose gene is situated upstream from the ptb gene, activated its expression.  |l eng 
593 |a Departamento de Química Biológica, Ciudad Universitaria Pabellón 2, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina 
690 1 0 |a BACTERIAL ENZYME 
690 1 0 |a ISOLEUCINE 
690 1 0 |a PHOSPHOTRANSBUTYRYLASE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a VALINE 
690 1 0 |a ARTICLE 
690 1 0 |a BACILLUS MEGATERIUM 
690 1 0 |a BACTERIAL GROWTH 
690 1 0 |a ENZYME ACTIVITY 
690 1 0 |a ENZYME SUBSTRATE 
690 1 0 |a GENE EXPRESSION REGULATION 
690 1 0 |a NONHUMAN 
690 1 0 |a NUCLEOTIDE SEQUENCE 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a REGULATORY MECHANISM 
690 1 0 |a AMINO ACIDS, BRANCHED-CHAIN 
690 1 0 |a BACILLUS MEGATERIUM 
690 1 0 |a CULTURE MEDIA 
690 1 0 |a DNA-BINDING PROTEINS 
690 1 0 |a DNA-DIRECTED RNA POLYMERASES 
690 1 0 |a GENE EXPRESSION REGULATION, BACTERIAL 
690 1 0 |a KINETICS 
690 1 0 |a PHOSPHATE ACETYLTRANSFERASE 
690 1 0 |a RNA POLYMERASE SIGMA 54 
690 1 0 |a SIGMA FACTOR 
690 1 0 |a BACILLUS MEGATERIUM 
690 1 0 |a BACTERIA (MICROORGANISMS) 
690 1 0 |a POSIBACTERIA 
690 1 0 |a PROKARYOTA 
700 1 |a Pettinari, M.J. 
700 1 |a Méndez, B.S. 
773 0 |d 2001  |g v. 42  |h pp. 345-349  |k n. 5  |p Curr. Microbiol.  |x 03438651  |w (AR-BaUEN)CENRE-160  |t Current Microbiology 
856 4 1 |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035078620&doi=10.1007%2fs002840010227&partnerID=40&md5=d94e6042037ba9d0d5e995ad71090ae8  |y Registro en Scopus 
856 4 0 |u https://doi.org/10.1007/s002840010227  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_03438651_v42_n5_p345_Vazquez  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v42_n5_p345_Vazquez  |y Registro en la Biblioteca Digital 
961 |a paper_03438651_v42_n5_p345_Vazquez  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
999 |c 63000 

Ejemplares similares