The role of phospholipids in the activity of a4-3-KET0steroid 5αoxidoreductase of the rat ventral prostate
The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis produc...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Informa Healthcare
1977
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | The involvement of phospholipids in the activity of 5αreductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5αreductase in both subcellular fractions; this effect was not attributable to the hydrolysis products. Phospholipase C stopped only the activity of the nuclear fraction. There was no quantitative correlation between hydrolysis of phospholipids and loss of enzyme activity. The inactivation observed seems to result from phospholipase-induced instability of the enzyme. The results suggest that the altered structure of phospholipids and/or the membranes where 5αreductase is located is responsible for the decrease in activity. © 1977 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
|---|---|
| Bibliografía: | Wilson, J.D., Gloyna, R.E., (1970) Rectn. Progr. Hormone Res., 26, p. 309 Frederiksen, D.W., Wilson, J.D., (1971) J. Biol. Chem, 246, p. 2584 Shirmazaki, J., Horaguchi, T., Ohki, J., Shida, K., (1971) Endocrinol. Jap., 18, p. 179 Moore, R.J., Wilson, J.D., (1972) J. Biol. Chem., 247, p. 958 Norzer, D., Tamaoki, B.I., (1974) Biochim. Biophys. Acta, 348, p. 321 Emmelot, P., Bos, C.J., (1968) Biochim. Biophys. Acta., 150, p. 341 Rethy, A., Tomasi, V., Trevisani, A., (1971) Arch. Biochem, Biophys., 147, p. 36 Beaufay, H., de Duve, C., (1954) Bull. Soc. Chim. Biol., 36, p. 1551 Duttera, S.M., Byrne, W.L., Ganoza, M.C., (1968) J. Biol. Chem., 243, p. 2216 Graham, A.B., Wood, G.L., (1969) Biochem. Biophys. Res. Commun., 37, p. 567 Zakim, D., (1970) J. Biol. Chem., 245, p. 4953 Charreau, E.H., Villee, C.A., (1968) J. Clin. Endocr. and Metab., 28, p. 1741 Saito, K., Hanahan, D.J., (1962) Biochemistry, 1, p. 521 Folch, J., Lees, M., Sloane Stanley, G.H., (1957) J. Biol. Chem., 226, p. 497 Borgstrom, B., (1952) Acta Physiol. Scand., 25, p. 101 Bartlett, G.R., (1959) J. Biol. Chem., 234, p. 466 Dole, V.P., (1956) J. Clin. Invest., 35, p. 150 Lowry, O.H., Rosbrough, J.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, p. 265 Felischer, S., Casu, A., Fleishcher, B., (1964) Fed. Proc., 23, p. 486 Casu, A., Fleischer, B., Fleischer, S., (1966) Fed. Proc., 25, pp. 3-4 Maronosi, A., (1964) Fed. Proc., 23, p. 913 Igo, R.P., Mackler, B., Ducan, J., Ridyard, J.N.A., Hanahan, P.J., (1960) Biochem. Biophys. Acta., 42, p. 55 Martonosi, A., (1963) Biochem. Biophys. Res. Commun., 13, p. 273 Fiscus, W.G., Schneider, W.C., (1966) J. Biol. Chem., 241, p. 3324 Goodman, D.S., (1958) J. Amer. Chem. Soc., 80, p. 3892 Verhoeven, D.S., Lamberigts, G., De Moor, P., (1974) J. Steroid Biochem., 5, p. 93 Salmoral, E.M., Charreau, E.H., (1974) VIII Pan American. Endocrinol. Meet. Abstr., 56 |
| ISSN: | 07435800 |
| DOI: | 10.3109/07435807709073915 |