Cyclic nucleotide phosphodiesterase activities in rat erythrocytes
Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Martinus Nijhoff/Dr. W. Junk Publishers
1982
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic GMP as substrate. When the system is in the 'non-aggregated state', no activity with cyclic GMP is detected. Conversion to the 'non-aggregated state' is enhanced by dilution or by addition of magnesium ions. Upon isoelectric focusing of erythrocyte soluble extracts, phosphodiesterase activities can be resolved in two peaks: one specific for cyclic AMP located at pH 4.66 and the other specific for cyclic GMP focused at pH 4.95. Evidence would indicate that aggregation affects the enzyme specific for cyclic AMP. © 1982 Martinus Nijhoff/Dr W. Junk Publishers. |
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| ISSN: | 03008177 |
| DOI: | 10.1007/BF00223007 |