Cyclic nucleotide phosphodiesterase activities in rat erythrocytes

Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic...

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Autor principal: Téllez-Iñón, M.T
Otros Autores: Torres, H.N
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Martinus Nijhoff/Dr. W. Junk Publishers 1982
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0019948006 
024 7 |2 cas  |a cyclic AMP, 60-92-4; cyclic GMP, 7665-99-8; cyclic nucleotide phosphodiesterase, 50812-31-2; 3',5'-Cyclic-GMP Phosphodiesterase, EC 3.1.4.35; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Magnesium Chloride, 7786-30-3; Magnesium, 7439-95-4 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a MCBIB 
100 1 |a Téllez-Iñón, M.T. 
245 1 0 |a Cyclic nucleotide phosphodiesterase activities in rat erythrocytes 
260 |b Martinus Nijhoff/Dr. W. Junk Publishers  |c 1982 
270 1 0 |m Téllez-Iñón, M.T.; Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Obligado 2490, Buenos Aires, 1428, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Wells, J.N., Hardman, J.G., (1977) Adv. Cyclic Nucl. Res., 8, pp. 119-143 
504 |a Strada, S.J., Thompson, W.J., (1978) Adv. Cyclic Nucl. Res., 9, pp. 265-283 
504 |a Pichard, A.L., Cheung, W.Y., (1976) J. Biol. Chem., 251, pp. 5726-5737 
504 |a Guan, W.R., Cheung, W.Y., (1980) Arch. Biochem. Biophys., 204, pp. 191-198 
504 |a Bylund, D.B., Téllez-Iñón, M.T., Hollenberg, M.D., (1977) Life Sci., 21, pp. 403-410 
504 |a Thompson, W.J., Appleman, M.M., (1971) Biochemistry, 10, pp. 311-316 
504 |a Londesborough, J., (1976) Anal. Biochem., 71, pp. 623-629 
504 |a Patterson, W.O., Hardman, J.G., Sutherland, E.W., (1976) Mol. Cell. Endocrinol., 5, pp. 51-56 
504 |a Segel, I.H., (1975) Enzyme Kinetics, pp. 213-214. , Wiley Interscience, New York, U.S.A 
504 |a Schröder, J., Rickenberg, H.V., (1973) Biochim. Biophys. Acta, 302, pp. 50-63 
504 |a Dicou, E.L., Bracet, P., (1979) Biochim. Biophys. Acta, 578, pp. 232-242 
520 3 |a Erythrocyte soluble phosphodiesterase activities show at least two interconvertible states: 'aggregated' and 'non-aggregated'. In the former state the existence of a high affinity component for cyclic AMP is favoured and the system is more active with cyclic AMP than with cyclic GMP as substrate. When the system is in the 'non-aggregated state', no activity with cyclic GMP is detected. Conversion to the 'non-aggregated state' is enhanced by dilution or by addition of magnesium ions. Upon isoelectric focusing of erythrocyte soluble extracts, phosphodiesterase activities can be resolved in two peaks: one specific for cyclic AMP located at pH 4.66 and the other specific for cyclic GMP focused at pH 4.95. Evidence would indicate that aggregation affects the enzyme specific for cyclic AMP. © 1982 Martinus Nijhoff/Dr W. Junk Publishers.  |l eng 
593 |a Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Obligado 2490, Buenos Aires, 1428, Argentina 
690 1 0 |a CYCLIC AMP 
690 1 0 |a CYCLIC GMP 
690 1 0 |a CYCLIC NUCLEOTIDE PHOSPHODIESTERASE 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a BLOOD AND HEMOPOIETIC SYSTEM 
690 1 0 |a ERYTHROCYTE 
690 1 0 |a RAT 
690 1 0 |a 3',5'-CYCLIC-GMP PHOSPHODIESTERASE 
690 1 0 |a 3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 
690 1 0 |a ANIMAL 
690 1 0 |a CHROMATOGRAPHY, GEL 
690 1 0 |a ERYTHROCYTES 
690 1 0 |a ISOELECTRIC FOCUSING 
690 1 0 |a KINETICS 
690 1 0 |a MAGNESIUM 
690 1 0 |a MAGNESIUM CHLORIDE 
690 1 0 |a MALE 
690 1 0 |a RATS 
690 1 0 |a RATS, INBRED STRAINS 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
700 1 |a Torres, H.N. 
773 0 |d Martinus Nijhoff/Dr. W. Junk Publishers, 1982  |g v. 43  |h pp. 161-166  |k n. 3  |p Mol Cell Biochem  |x 03008177  |w (AR-BaUEN)CENRE-3498  |t Molecular and Cellular Biochemistry 
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