Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii

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Cell wall fragments from both yeast-like and mycelial forms of the dimorphic fungus Mucor rouxii were used as enzymatic preparations to study the synthesis and role of prenyl-phospho-sugars in these systems. In the presence of GDP ['4C] mannose two main products were formed. One of them was cha...

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Detalles Bibliográficos
Autor principal: Bernard, E.A
Otros Autores: Guaragna, R., Amaral, B.B, Perry, M.L.S, Pereira, I.R.G, Ielpi, L., Couso, R.O
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Martinus Nijhoff/Dr. W. Junk Publishers 1982
Acceso en línea:Registro en Scopus
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 scopus  |a 2-s2.0-0020492757 
024 7 |2 cas  |a glucose, 50-99-7, 84778-64-3; lipid, 66455-18-3; mannose, 31103-86-3, 3458-28-4; Glucose, 50-99-7; Lipids; Mannose, 31103-86-3; Polysaccharides 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a MCBIB 
100 1 |a Bernard, E.A. 
245 1 0 |a Formation of lipid-linked sugars in mycelial and yeast-like forms of Mucor rouxii 
260 |b Martinus Nijhoff/Dr. W. Junk Publishers  |c 1982 
270 1 0 |m Bernard, E.A.; Departamento de Bioquimica, Instituto de Biociéncias UFRGS, Porto Alegre, 90000, Brazil 
506 |2 openaire  |e Política editorial 
504 |a Lennarz, W.J., Scher, M.G., (1972) Biochim. Biophys. Acta, 265, pp. 417-441 
504 |a Staneloni, R.J., Leloir, L.F., (1979) Trends Biochem. Sci., 4, pp. 65-67 
504 |a Letoublon, R.C.P., Comte, J., Got, R., (1973) Eur. J. Biochem., 40, pp. 95-101 
504 |a Gold, M.H., Hahn, H.J., (1976) Biochemistry, 15, pp. 1808-1814 
504 |a Couso, R.O., Ielpi, L., Garcia, R.C., Dankert, M., (1980) Arch. Biochem. Biophys., 204, pp. 434-443 
504 |a Garcia, R.C., Recondo, E., Dankert, M., (1974) Eur. J. Biochem., 43, pp. 93-105 
504 |a Romero, P., Garcia, R.C., Dankert, M., (1977) Mol. Cell. Biochem., 16, pp. 205-212 
504 |a Pont Lezica, R., Romero, P.A., Dankert, M.A., (1976) Plant Physiol., 58, pp. 675-680 
504 |a Bartnicki-Garcia, S., Nickerson, W.J., (1962) J. Bacteriol., 84, pp. 829-840 
504 |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., (1951) J. Biol. Chem., 193, pp. 265-275 
504 |a Arima, T., Spiro, M.J., Spiro, R.G., (1972) J. Biol. Chem., 247, pp. 1825-1835 
504 |a Dankert, M., Wright, A., Kelly, W.S., Robbins, P.W., (1966) Arch. Biochem. Biophys., 116, pp. 425-435 
504 |a Trevelyan, W.E., Procter, D.P., Harrison, J.S., (1950) Nature (Lond.), 166, pp. 444-445 
504 |a Herscovics, A., Warren, C.D., Jeanloz, R.W., Wedgwood, J.F., Liu, I.Y., Strominger, J.L., (1974) FEBS Lett., 45, pp. 312-317 
504 |a Pont Lezica, R., Brett, C.T., Romero Martinez, P., Dankert, M.A., (1975) Biochem. Biophys. Res. Commun., 66, pp. 980-987 
504 |a Morrison, W.R., (1969) Biochim. Biophys. Acta, 176, pp. 537-546 
504 |a Krisman, C.R., (1962) Analyt. Biochem., 4, pp. 17-23 
504 |a Dow, J.M., Rubery, P.H., (1977) J. Gen. Microbiol., 99, pp. 29-41 
504 |a Jung, P., Tanner, W., Identification of the Lipid Intermediate in Yeast Mannan Biosynthesis (1973) European Journal of Biochemistry, 37, pp. 1-6 
504 |a Gibson, R., Kornfeld, S., Schlesinger, S., A role for oligosaccharides in glycoprotein biosynthesis (1980) Trends in Biochemical Sciences, 5, pp. 290-293 
520 3 |a Cell wall fragments from both yeast-like and mycelial forms of the dimorphic fungus Mucor rouxii were used as enzymatic preparations to study the synthesis and role of prenyl-phospho-sugars in these systems. In the presence of GDP ['4C] mannose two main products were formed. One of them was characterized as dolichol-monophosphate β-mannose on the following basis: solubility in organic solvents, behaviour upon paper chromatography, DEAF cellulose column chromatography, mild acid hydrolysis, alkali treatment, catalytic reduction and phenol degradation. The other product was identified as a glicoprotein containing a single mannose unit linked to a serine or threonine residue. It was degraded with pronase and by mild NaOH-NaBH4 treatment all the radioactivity was released as free mannitol. When UDP[4C] glucose was employed as sugar donor two butanol soluble components were isolated. One of them (25%) was characterized as dolichol-monophosphate-ß-glucose on the basis of the same criteria as described above. The other one (75%) was neutral and was not studied in detail. Mycelial enzymes were about 40 times more active in the synthesis of the dolichol derivatives. In addition, large amounts of glycogen were detected. The role that both dolichol derivatives might play in glycoprotein biosynthesis is discussed. © 1982 Martinus Nijhoff/Dr W. Junk Publishers.  |l eng 
593 |a Departamento de Bioquimica, Instituto de Biociéncias UFRGS, Porto Alegre, 90000, Brazil 
593 |a Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Buenos Aires, 1428, Argentina 
690 1 0 |a GLUCOSE 
690 1 0 |a LIPID 
690 1 0 |a MANNOSE 
690 1 0 |a POLYSACCHARIDE 
690 1 0 |a ARTICLE 
690 1 0 |a BIOSYNTHESIS 
690 1 0 |a CELL WALL 
690 1 0 |a CHEMISTRY 
690 1 0 |a METABOLISM 
690 1 0 |a MUCOR 
690 1 0 |a CELL WALL 
690 1 0 |a CHEMISTRY 
690 1 0 |a GLUCOSE 
690 1 0 |a LIPIDS 
690 1 0 |a MANNOSE 
690 1 0 |a MUCOR 
690 1 0 |a POLYSACCHARIDES 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
700 1 |a Guaragna, R. 
700 1 |a Amaral, B.B. 
700 1 |a Perry, M.L.S. 
700 1 |a Pereira, I.R.G. 
700 1 |a Ielpi, L. 
700 1 |a Couso, R.O. 
773 0 |d Martinus Nijhoff/Dr. W. Junk Publishers, 1982  |g v. 45  |h pp. 41-48  |k n. 1  |p Mol Cell Biochem  |x 03008177  |w (AR-BaUEN)CENRE-3498  |t Molecular and Cellular Biochemistry 
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856 4 0 |u https://doi.org/10.1007/BF01283162  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_03008177_v45_n1_p41_Bernard  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v45_n1_p41_Bernard  |y Registro en la Biblioteca Digital 
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962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
963 |a VARI 
999 |c 79349 

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