Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi
A cyclic AMP-dependent protein kinase activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns, giving two peaks of kinase activity, which were eluted at 0.15 M- and 0.32 M-NaCl respectively. The second activity peak w...
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| Otros Autores: | , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1988
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| Acceso en línea: | Registro en Scopus Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 03618caa a22006017a 4500 | ||
|---|---|---|---|
| 001 | PAPER-18040 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204924.0 | ||
| 008 | 190411s1988 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0023764268 | |
| 024 | 7 | |2 cas |a cyclic AMP, 60-92-4; protein kinase, 9026-43-1; Cyclic AMP, 60-92-4; Phosphates; Protein Kinases, EC 2.7.1.37 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BIJOA | ||
| 100 | 1 | |a Ulloa, R.M. | |
| 245 | 1 | 0 | |a Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi |
| 260 | |c 1988 | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a A cyclic AMP-dependent protein kinase activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns, giving two peaks of kinase activity, which were eluted at 0.15 M- and 0.32 M-NaCl respectively. The second activity peak was stimulated by nanomolar concentrations of cyclic AMP. In addition, a cyclic AMP-binding protein co-eluted with the second kinase activity peak. Cyclic AMP-dependent protein kinase activity was further purified by gel filtration, affinity chromatography on histone-agarose and cyclic AMP-agarose, as well as by chromatography on CM-Sephadex. The enzyme ('holoenzyme') could be partially dissociated into two different components: 'catalytic' and 'regulatory'. The 'regulatory' component had specific binding for cyclic AMP, and it inhibited phosphotransferase activity of the homologous 'catalytic component' or of the 'catalytic subunit' from bovine heart. Cyclic AMP reversed these inhibitions. A 'holoenzyme preparation' was phosphorylated in the absence of exogenous phosphate acceptor and analyzed by polyacrylamide- gel electrophoresis. A 56 kDa band was phosphorylated. The same preparation was analysed by Western blotting, by using polyclonal antibodies to the regulatory subunits of protein kinases type I or II. Both antibodies reacted with the 56 kDa band. |l eng | |
| 593 | |a Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ANTIBODY |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a PROTEIN KINASE |
| 690 | 1 | 0 | |a IMMUNOBLOTTING |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTOZOON |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CATTLE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, AFFINITY |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, DEAE-CELLULOSE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, GEL |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a IMMUNOBLOTTING |
| 690 | 1 | 0 | |a MYOCARDIUM |
| 690 | 1 | 0 | |a PHOSPHATES |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PROTEIN BINDING |
| 690 | 1 | 0 | |a PROTEIN KINASES |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Mesri, E. | |
| 700 | 1 | |a Esteva, M. | |
| 700 | 1 | |a Torres, H.N. | |
| 700 | 1 | |a Tellez-Inon, M.T. | |
| 773 | 0 | |d 1988 |g v. 255 |h pp. 319-326 |k n. 1 |p BIOCHEM. J. |x 02646021 |w (AR-BaUEN)CENRE-205 |t Biochemical Journal | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023764268&partnerID=40&md5=5f2ea4d3b457f1d31227ac30c78b7258 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_02646021_v255_n1_p319_Ulloa |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v255_n1_p319_Ulloa |y Registro en la Biblioteca Digital |
| 961 | |a paper_02646021_v255_n1_p319_Ulloa |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 78993 | ||