a-1,4-glucan: a-1,4-glucan 6-glycosyltransferase from liver
a-1,4-glucan:a-1,4-glucan 6-glycosyltransferase was purified about 35-fold from rat-liver extracts. Removal of α-amylase could be accomplished by high-speed centrifugation of extracts from livers with high glycogen content. Enzyme action on amylopectin is optimum at pH 6.4 in 0.3 M citrate buffer. I...
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| Autor principal: | |
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1962
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 03373caa a22004337a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1771 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203106.0 | ||
| 008 | 190411s1962 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-9844232718 | |
| 024 | 7 | |2 cas |a glucosyltransferase, 9031-48-5; Glucosyltransferases, 2.4.1.- | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Krisman, C.R. | |
| 245 | 1 | 0 | |a a-1,4-glucan: a-1,4-glucan 6-glycosyltransferase from liver |
| 260 | |c 1962 | ||
| 270 | 1 | 0 | |m Krisman, C.R.; Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a a-1,4-glucan:a-1,4-glucan 6-glycosyltransferase was purified about 35-fold from rat-liver extracts. Removal of α-amylase could be accomplished by high-speed centrifugation of extracts from livers with high glycogen content. Enzyme action on amylopectin is optimum at pH 6.4 in 0.3 M citrate buffer. It requires salts for maximal activity, and is inhibited by molybdate, Mn2+, Mg2+ and sodium p-chloromercuribenzoate. Amylose and amylopectin ß-limit dextrin were also substrates for the liver branching enzyme. A method is described for the assay of the enzyme in the presence of α-amylase. © 1962. |l eng | |
| 536 | |a Detalles de la financiación: U.S. Public Health Service | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, T6cnicas | ||
| 536 | |a Detalles de la financiación: National Institutes of Health | ||
| 536 | |a Detalles de la financiación: Rockefeller Foundation | ||
| 536 | |a Detalles de la financiación: I wish to express my gratitude to Dr. L. F. LELOIR for his inspiring guidance and support, and to the other members of the Instituto de Investigaciones Bioquimicas "Fundaci6n Campomar" for many helpful discussions and criticisms, This investigation was supported in part by a research grant (No G-3442) from the National Institutes of Health, U.S. Public Health Service, by the Rockefeller Foundation and by the Consejo Nacional de Investigaciones Cientificas y T6cnicas. The author is an investigator of the Instituto Nacional de Microbiologla. | ||
| 593 | |a Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a GLUCOSYLTRANSFERASE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a GLUCOSYLTRANSFERASES |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a GLUCOSYLTRANSFERASES |
| 690 | 1 | 0 | |a LIVER |
| 773 | 0 | |d 1962 |g v. 65 |h pp. 307-315 |k n. 2 |x 00063002 |w (AR-BaUEN)CENRE-164 |t BBA - Biochimica et Biophysica Acta | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-9844232718&doi=10.1016%2f0006-3002%2862%2991049-1&partnerID=40&md5=33bb951be1bbeb772942e483cc778c49 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0006-3002(62)91049-1 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00063002_v65_n2_p307_Krisman |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063002_v65_n2_p307_Krisman |y Registro en la Biblioteca Digital |
| 961 | |a paper_00063002_v65_n2_p307_Krisman |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 62724 | ||