Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach
Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dy...
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Springer Verlag
2018
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| LEADER | 17176caa a22014777a 4500 | ||
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| 003 | AR-BaUEN | ||
| 005 | 20230518204824.0 | ||
| 008 | 190410s2018 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85033403418 | |
| 024 | 7 | |2 cas |a alcohol dehydrogenase, 9031-72-5; aldehyde dehydrogenase, 37353-37-0, 9028-86-8; alkane 1 monooxygenase, 9059-16-9; diesel fuel, 68334-30-5; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; oxygen, 7782-44-7; RNA, 63231-63-0 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JIMBF | ||
| 100 | 1 | |a Tribelli, P.M. | |
| 245 | 1 | 0 | |a Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
| 260 | |b Springer Verlag |c 2018 | ||
| 270 | 1 | 0 | |m Raiger Iustman, L.J.; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes, 2160, Argentina; email: lri@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13–C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments. © 2017, Society for Industrial Microbiology and Biotechnology. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: EMBO | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: Acknowledgements This work was partially supported by grants from UBA, CONICET, and ANPCyT. PMT, MMR, LJRI and NIL are career investigators from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina). RNA-seq experiments were performed by PMT at Dr. Molin’s Lab supported by a short term EMBO fellowship. Authors want to thank to anonymous reviewers for the helpful criticisms. | ||
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes, 2160, Buenos Aires, C1428EGA, Argentina | ||
| 593 | |a IQUIBICEN, CONICET, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina | ||
| 593 | |a Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark | ||
| 690 | 1 | 0 | |a ALKANE DEGRADATION |
| 690 | 1 | 0 | |a ALKB |
| 690 | 1 | 0 | |a MICRO-AEROBIOSIS |
| 690 | 1 | 0 | |a PSEUDOMONAS EXTREMAUSTRALIS |
| 690 | 1 | 0 | |a RNA-SEQ |
| 690 | 1 | 0 | |a ALCOHOL DEHYDROGENASE |
| 690 | 1 | 0 | |a ALDEHYDE DEHYDROGENASE |
| 690 | 1 | 0 | |a ALKANE |
| 690 | 1 | 0 | |a ALKANE 1 MONOOXYGENASE |
| 690 | 1 | 0 | |a DIESEL FUEL |
| 690 | 1 | 0 | |a HYDROCARBON |
| 690 | 1 | 0 | |a OXIDOREDUCTASE |
| 690 | 1 | 0 | |a OXYGEN |
| 690 | 1 | 0 | |a RNA |
| 690 | 1 | 0 | |a RUBREDOXIN |
| 690 | 1 | 0 | |a RUBREDOXINE REDUCTASE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a AEROBIC METABOLISM |
| 690 | 1 | 0 | |a AMINO ACID METABOLISM |
| 690 | 1 | 0 | |a ARGC GENE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a AZU GENE |
| 690 | 1 | 0 | |a BACTERIAL COUNT |
| 690 | 1 | 0 | |a CARBON SOURCE |
| 690 | 1 | 0 | |a CELL VIABILITY |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DEGRADATION |
| 690 | 1 | 0 | |a DENITRIFICATION |
| 690 | 1 | 0 | |a DOWN REGULATION |
| 690 | 1 | 0 | |a FLGA GENE |
| 690 | 1 | 0 | |a GENE |
| 690 | 1 | 0 | |a GENE EXPRESSION |
| 690 | 1 | 0 | |a GENETIC ORGANIZATION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PROMOTER REGION |
| 690 | 1 | 0 | |a PSEUDOMONAS |
| 690 | 1 | 0 | |a PSEUDOMONAS EXTREMAUSTRALIS |
| 690 | 1 | 0 | |a REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION |
| 690 | 1 | 0 | |a RNA SEQUENCE |
| 690 | 1 | 0 | |a TRANSCRIPTOMICS |
| 690 | 1 | 0 | |a UPREGULATION |
| 700 | 1 | |a Rossi, L. | |
| 700 | 1 | |a Ricardi, M.M. | |
| 700 | 1 | |a Gomez-Lozano, M. | |
| 700 | 1 | |a Molin, S. | |
| 700 | 1 | |a Raiger Iustman, L.J. | |
| 700 | 1 | |a Lopez, N.I. | |
| 773 | 0 | |d Springer Verlag, 2018 |g v. 45 |h pp. 15-23 |k n. 1 |p J. Ind. Microbiol. Biotechnol. |x 13675435 |t Journal of Industrial Microbiology and Biotechnology | |
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