RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella
Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response. This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activit...
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Oxford University Press
2018
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 10790caa a22011777a 4500 | ||
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| 001 | PAPER-17053 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518204810.0 | ||
| 008 | 190410s2018 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85048107543 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JIDIA | ||
| 100 | 1 | |a Valguarnera, E. | |
| 245 | 1 | 0 | |a RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella |
| 260 | |b Oxford University Press |c 2018 | ||
| 270 | 1 | 0 | |m Ugalde, J.E.; Instituto de Investigaciones Biotecnológicas dr. Rodolfo A. Ugalde, IIB-INTECH, CONICET, Universidad Nacional de San Martín, Campus Miguelete, UNSAM, Avenida 25 de Mayo y Francia, Argentina; email: jugalde@iibintech.com.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Salcedo, S.P., Marchesini, M.I., Lelouard, H., Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1 (2008) PLoS Pathog, 4, p. e21 | ||
| 504 | |a Spera, J.M., Comerci, D.J., Ugalde, J.E., Brucella alters the immune response in a prpA-dependent manner (2014) Microb Pathog, 67-68, pp. 8-13 | ||
| 504 | |a Spera, J.M., Herrmann, C.K., Roset, M.S., Comerci, D.J., Ugalde, J.E., A Brucella virulence factor targets macrophages to trigger B-cell proliferation (2013) J Biol Chem, 288, pp. 20208-20216 | ||
| 504 | |a Spera, J.M., Ugalde, J.E., Mucci, J., Comerci, D.J., Ugalde, R.A., A B lymphocyte mitogen is a Brucella abortus virulence factor required for persistent infection (2006) Proc Natl Acad Sci USA, 103, pp. 16514-16519 | ||
| 504 | |a Martirosyan, A., Moreno, E., Gorvel, J.P., An evolutionary strategy for a stealthy intracellular Brucella pathogen (2011) Immunol Rev, 240, pp. 211-234 | ||
| 504 | |a Ruiz, N., Kahne, D., Silhavy, T.J., Transport of lipopolysaccha-ride across the cell envelope: The long road of discovery (2009) Nat Rev Microbiol, 7, pp. 677-683 | ||
| 504 | |a Okuda, S., Sherman, D.J., Silhavy, T.J., Ruiz, N., Kahne, D., Lipopolysaccharide transport and assembly at the outer mem-brane: The PEZ model (2016) Nat Rev Microbiol, 14, pp. 337-345 | ||
| 504 | |a Conde-Álvarez, R., Arce-Gorvel, V., Iriarte, M., The lipo-polysaccharide core of Brucella abortus acts as a shield against innate immunity recognition (2012) PLoS Pathog, 8, p. e1002675 | ||
| 504 | |a Döhmer, P.H., Valguarnera, E., Czibener, C., Ugalde, J.E., Identifcation of a type IV secretion substrate of Brucella abortus that participates in the early stages of intracellular survival (2014) Cell Microbiol, 16, pp. 396-410 | ||
| 504 | |a Roset, M.S., García Fernández, L., DelVecchio, V.G., Briones, G., Intracellularly induced cyclophilins play an important role in stress adaptation and virulence of Brucella abortus (2013) Infect Immun, 81, pp. 521-530 | ||
| 504 | |a Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purifcation (1959) Can J Biochem Physiol, 37, pp. 911-917 | ||
| 504 | |a White, P.G., Wilson, J.B., Diferentiation of smooth and nons-mooth colonies of Brucellae (1951) J Bacteriol, 61, pp. 239-240 | ||
| 504 | |a Luederitz, O., Risse, H.J., Schulte-Holthausen, H., Strominger, J.L., Sutherland, I.W., Westphal, O., Biochemical studies of the smooth-rough mutation in Salmonella Minnesota (1965) J Bacteriol, 89, pp. 343-354 | ||
| 504 | |a Ugalde, J.E., Czibener, C., Feldman, M.F., Ugalde, R.A., Identifcation and characterization of the Brucella abortus phosphoglucomutase gene: Role of lipopolysaccharide in virulence and intracellular multiplication (2000) Infect Immun, 68, pp. 5716-5723 | ||
| 504 | |a Ugalde, J.E., Comerci, D.J., Leguizamón, M.S., Ugalde, R.A., Evaluation of Brucella abortus phosphoglucomutase (pgm) mutant as a new live rough-phenotype vaccine (2003) Infect Immun, 71, pp. 6264-6269 | ||
| 504 | |a Guidolin, L.S., Morrone Seijo, S.M., Guaimas, F.F., Comerci, D.J., Ciocchini, A.E., Interaction network and localization of Brucella abortus membrane proteins involved in the synthesis, transport, and succinylation of cyclic β-1, 2-glucans (2015) J Bacteriol, 197, pp. 1640-1648 | ||
| 504 | |a Marchesini, M.I., Ugalde, J.E., Czibener, C., Comerci, D.J., Ugalde, R.A., N-terminal-capturing screening system for the isolation of Brucella abortus genes encoding surface exposed and secreted proteins (2004) Microb Pathog, 37, pp. 95-105 | ||
| 504 | |a Bos, M.P., Tefsen, B., Geurtsen, J., Tommassen, J., Identifcation of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface (2004) Proc Natl Acad Sci USA, 101, pp. 9417-9422 | ||
| 504 | |a Dinh, T., Bernhardt, T.G., Using superfolder green fuores-cent protein for periplasmic protein localization studies (2011) J Bacteriol, 193, pp. 4984-4987 | ||
| 504 | |a West, N.P., Sansonetti, P., Mounier, J., Optimization of virulence functions through glucosylation of Shigella LPS (2005) Science, 307, pp. 1313-1317 | ||
| 504 | |a Terwagne, M., Ferooz, J., Rolán, H.G., Innate immune recognition of fagellin limits systemic persistence of Brucella (2013) Cell Microbiol, 15, pp. 942-960 | ||
| 520 | 3 | |a Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response. This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities. Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response. We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane. A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation. We show that RomA is involved in the synthesis of LPS, probably coordinating part of the biosynthetic complex in the periplasm. Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype. Our results suggest that the proper synthesis of LPS is central to maximize virulence and minimize inflammation. © 2017 The Author(s). |l eng | |
| 536 | |a Detalles de la financiación: National Council for Scientific Research | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgments. We thank members of the J.E.U. laboratory for useful discussions and Thomas Bernhardt from Harvard University for kindly providing the superfolder GFP coding plasmid. C. C., S. G. A., A. S. C., K. A. P., J. M. S., J. C. and J. E. U. are members of the National Research Council of Argentina (CONICET). Financial support. This work was supported by grants PICT-PRH08-160 to C. C., PICT-PRH08-230 and PICT-1028-2014 to J. E. U., and grants from the University of San Martín to C. C. and J. E. U. E.V. was supported by a fellowship of the National Research Council of Argentina (CONICET). | ||
| 593 | |a Instituto de Investigaciones Biotecnológicas dr. Rodolfo A. Ugalde, IIB-INTECH, CONICET, Universidad Nacional de San Martín, Campus Miguelete, UNSAM, Avenida 25 de Mayo y Francia, San Martín (1650), Buenos Aires, Argentina | ||
| 593 | |a Facultad de Ciencias Exactas y Naturales, Departamento de Quimica Organica-Consejo Nacional de Lnvestigaciones Cientificas y Tecnicas, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Biología Molecular y Celular de Rosario (IBR), Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina | ||
| 690 | 1 | 0 | |a BRUCELLA |
| 690 | 1 | 0 | |a INFLAMMATION |
| 690 | 1 | 0 | |a LIPOPOLYSACCHARIDE |
| 690 | 1 | 0 | |a LIPOPOLYSACCHARIDE |
| 690 | 1 | 0 | |a PERIPLASMIC PROTEIN |
| 690 | 1 | 0 | |a ROMA PROTEIN |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ALPHAPROTEOBACTERIA |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BACTERIAL GENE |
| 690 | 1 | 0 | |a BACTERIAL OUTER MEMBRANE |
| 690 | 1 | 0 | |a BACTERIAL STRAIN |
| 690 | 1 | 0 | |a BACTERIAL VIRULENCE |
| 690 | 1 | 0 | |a BACTERIUM ADHERENCE |
| 690 | 1 | 0 | |a BIOSYNTHESIS |
| 690 | 1 | 0 | |a BRUCELLA ABORTUS |
| 690 | 1 | 0 | |a BRUCELLOSIS |
| 690 | 1 | 0 | |a CARBOHYDRATE ANALYSIS |
| 690 | 1 | 0 | |a CARBOHYDRATE SYNTHESIS |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a INFLAMMATION |
| 690 | 1 | 0 | |a MACROMOLECULE |
| 690 | 1 | 0 | |a MEMBRANE DAMAGE |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a MUTANT |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PERIPLASM |
| 690 | 1 | 0 | |a PHENOTYPE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN CARBOHYDRATE INTERACTION |
| 690 | 1 | 0 | |a PROTEIN DETERMINATION |
| 690 | 1 | 0 | |a PROTEIN FUNCTION |
| 650 | 1 | 7 | |2 spines |a HOMEOSTASIS |
| 700 | 1 | |a Spera, J.M. | |
| 700 | 1 | |a Czibener, C. | |
| 700 | 1 | |a Fulgenzi, F.R. | |
| 700 | 1 | |a Casabuono, A.C. | |
| 700 | 1 | |a Altabe, S.G. | |
| 700 | 1 | |a Pasquevich, K.A. | |
| 700 | 1 | |a Guaimas, F. | |
| 700 | 1 | |a Cassataro, J. | |
| 700 | 1 | |a Couto, A.S. | |
| 700 | 1 | |a Ugalde, J.E. | |
| 773 | 0 | |d Oxford University Press, 2018 |g v. 217 |h pp. 1257-1266 |k n. 8 |p J. Infect. Dis. |x 00221899 |w (AR-BaUEN)CENRE-5621 |t Journal of Infectious Diseases | |
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