Nucleotides and nucleotide sugars in human blood cells. II. Group "O"
A study of nucleotides and nucleotide sugars in human blood cells has been carried out. Ten samples from healthy yound people of group "O" were analyzed by ion-exchange chromatography, and the following nucleotides and nucleotide sugars were found: NAD+; UDP-glucose, UDP-mannose, UDP-galac...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1971
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 10094caa a22015497a 4500 | ||
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| 001 | PAPER-1551 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203054.0 | ||
| 008 | 190411s1971 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0014992877 | |
| 024 | 7 | |2 cas |a alcohol, 64-17-5; ammonium chloride, 12125-02-9; carbon, 7440-44-0; chloroform, 67-66-3; fucose, 3615-37-0, 3713-31-3; galactose, 26566-61-0, 50855-33-9, 59-23-4; glucosamine, 3416-24-8, 4607-22-1; hexose, 93780-23-5; mannose, 31103-86-3, 3458-28-4; nicotinamide adenine dinucleotide phosphate, 53-59-8; nicotinamide adenine dinucleotide, 53-84-9; trichloroacetic acid, 14357-05-2, 76-03-9; ABO Blood-Group System; Adenine Nucleotides; Ammonium Chloride, 12125-02-9; Blood Glucose; Carbon Isotopes; Chloroform, 67-66-3; Ethanol, 64-17-5; Fucose, 3713-31-3; Galactose, 26566-61-0; Glucosamine, 3416-24-8; Guanine Nucleotides; Hexosamines; Hexoses; Mannose, 31103-86-3; NAD, 53-84-9; NADP, 53-59-8; Nucleoside Diphosphate Sugars; Nucleotides; Trichloroacetic Acid, 76-03-9; Uracil Nucleotides | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BBGSB | ||
| 100 | 1 | |a Cantore, M.L. | |
| 245 | 1 | 0 | |a Nucleotides and nucleotide sugars in human blood cells. II. Group "O" |
| 260 | |c 1971 | ||
| 270 | 1 | 0 | |m Recondo, E.F.; Instituto de Investigaciones Bioquímicas Fundacion CampomarArgentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Gerlach, Fleckenstein, Gross, (1958) Arch. Ges. Physiol., 266, p. 528 | ||
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| 520 | 3 | |a A study of nucleotides and nucleotide sugars in human blood cells has been carried out. Ten samples from healthy yound people of group "O" were analyzed by ion-exchange chromatography, and the following nucleotides and nucleotide sugars were found: NAD+; UDP-glucose, UDP-mannose, UDP-galactose, UDP-acetylglucosamine and UDP-acetylgalactosamine; AMP; NADP+; ADP-glucose, ADP-galactose and ADP-mannose; GDP-mannose and GDP-fucose; ADP and ATP. The sugar nucleotides were identified on the basis of chemical, enzymatic and chromatographic analysis. UDP-galactose, UDP-mannose, UDP-acetylgalactosamine, ADP-glucose, ADP-galactose, ADP-mannose, GDP-mannose and GDP-fucose have not been found before in human blood, and UDP-mannose was not known to occur in nature. In order to minimize the decomposition of unstable substances, mild techniques were applied: cold acid extraction and neutral (ethanol-chloroform) extraction were compared, and in both cases the aqueous phase was passed through a column of Dowex-i (Cl-form) resin, and the nucleotides eluted with a linear gradient of NH4Cl. Results showed that the extraction step is not very critical, but the use of ammonium salts and almost neutral pH, as compared with methods used in previous papers, permitted the isolation of ten nucleotide-linked sugars, eight of which have not been described before in human blood. An adenine nucleotide with very particular properties was extracted with the ethanol-chloroform technique; it was eluted by the concentration of NH4Cl necessary to elute ATP, but it behaved as AMP on paper chromatography in several solvent systems. It is very unstable and decomposes even at -20° in a few days. © 1971. |l eng | |
| 536 | |a Detalles de la financiación: U.S. Public Health Service, GM o3442 | ||
| 536 | |a Detalles de la financiación: Universidad Nacional del Centro de la Provincia de Buenos Aires, MACTUR I-BS | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, T6cnicas | ||
| 536 | |a Detalles de la financiación: National Institutes of Health | ||
| 536 | |a Detalles de la financiación: The authors wish to express their gratitude to Dr. Luis F. Leloir and Dr. M. Dankert for helpful suggestions and criticisms and to the other members of the Insti-tuto de Investigaciones Bioquimicas for their collaboration. They are also indebted to Mr. Manuel Hern~ndez for skillful technical assistance and to the students of the Instituto Nacional de Educaci6n Fisica de Buenos Aires who were the blood donors. This investigation was supported by grants from the National Institutes of Health, U.S. Public Health Service (No. GM o3442), the Consejo Nacional de Investigaciones Cientificas y T6cnicas (R. Argentina) and the Ministerio de Bienestar Social de la Provincia de Buenos Aires (MACTUR I-BS). M.L.C. and P.L. are Fellows of the Ministerio de Bienestar Social de la Provincia de Buenos Aires. A.F.L. is from the Departamento de Medicina de la Actividad Humana, Ministerio de Bienestar Social de la Provincia de Buenos Aires. E. F.R. is a Career Investigator of the Consejo Nacional de Investigaciones Cientifieas y T~cnicas (Argentina). | ||
| 593 | |a Instituto de Investigaciones Bioquímicas Fundacion Campomar, Argentina | ||
| 593 | |a Facultad de Ciencias Exactas y Naturales, Obligado 2490 Buenos Aires (28), Argentina | ||
| 593 | |a Ministerio de Bienestar Social de la Provincia de Buenos Aires Argentina | ||
| 690 | 1 | 0 | |a ADENINE NUCLEOTIDE |
| 690 | 1 | 0 | |a ALCOHOL |
| 690 | 1 | 0 | |a AMMONIUM CHLORIDE |
| 690 | 1 | 0 | |a CHLOROFORM |
| 690 | 1 | 0 | |a FUCOSE |
| 690 | 1 | 0 | |a GALACTOSE |
| 690 | 1 | 0 | |a GLUCOSAMINE |
| 690 | 1 | 0 | |a GUANINE NUCLEOTIDE |
| 690 | 1 | 0 | |a HEXOSAMINE |
| 690 | 1 | 0 | |a HEXOSE |
| 690 | 1 | 0 | |a MANNOSE |
| 690 | 1 | 0 | |a NICOTINAMIDE ADENINE DINUCLEOTIDE |
| 690 | 1 | 0 | |a NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE |
| 690 | 1 | 0 | |a NUCLEOSIDE DIPHOSPHATE SUGAR |
| 690 | 1 | 0 | |a NUCLEOTIDE |
| 690 | 1 | 0 | |a PYRIMIDINE NUCLEOTIDE |
| 690 | 1 | 0 | |a TRICHLOROACETIC ACID |
| 690 | 1 | 0 | |a ADULT |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BLOOD |
| 690 | 1 | 0 | |a BLOOD CELL |
| 690 | 1 | 0 | |a BLOOD GROUP ABO SYSTEM |
| 690 | 1 | 0 | |a ELECTROPHORESIS |
| 690 | 1 | 0 | |a GLUCOSE BLOOD LEVEL |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a ION EXCHANGE CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a PAPER |
| 690 | 1 | 0 | |a PAPER CHROMATOGRAPHY |
| 690 | 1 | 0 | |a SPECTROPHOTOMETRY |
| 690 | 1 | 0 | |a TECHNIQUE |
| 690 | 1 | 0 | |a ULTRAVIOLET RADIATION |
| 690 | 1 | 0 | |a ABO BLOOD-GROUP SYSTEM |
| 690 | 1 | 0 | |a ADENINE NUCLEOTIDES |
| 690 | 1 | 0 | |a ADULT |
| 690 | 1 | 0 | |a AMMONIUM CHLORIDE |
| 690 | 1 | 0 | |a BLOOD CELLS |
| 690 | 1 | 0 | |a BLOOD GLUCOSE |
| 690 | 1 | 0 | |a CARBON ISOTOPES |
| 690 | 1 | 0 | |a CHLOROFORM |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, ION EXCHANGE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, PAPER |
| 690 | 1 | 0 | |a ELECTROPHORESIS |
| 690 | 1 | 0 | |a ETHANOL |
| 690 | 1 | 0 | |a FUCOSE |
| 690 | 1 | 0 | |a GALACTOSE |
| 690 | 1 | 0 | |a GLUCOSAMINE |
| 690 | 1 | 0 | |a GUANINE NUCLEOTIDES |
| 690 | 1 | 0 | |a HEXOSAMINES |
| 690 | 1 | 0 | |a HEXOSES |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HYDROGEN-ION CONCENTRATION |
| 690 | 1 | 0 | |a MANNOSE |
| 690 | 1 | 0 | |a METHODS |
| 690 | 1 | 0 | |a NAD |
| 690 | 1 | 0 | |a NADP |
| 690 | 1 | 0 | |a NUCLEOSIDE DIPHOSPHATE SUGARS |
| 690 | 1 | 0 | |a NUCLEOTIDES |
| 690 | 1 | 0 | |a PAPER |
| 690 | 1 | 0 | |a SPECTROPHOTOMETRY |
| 690 | 1 | 0 | |a TRICHLOROACETIC ACID |
| 690 | 1 | 0 | |a ULTRAVIOLET RAYS |
| 690 | 1 | 0 | |a URACIL NUCLEOTIDES |
| 650 | 1 | 7 | |2 spines |a CARBON |
| 650 | 1 | 7 | |2 spines |a PH |
| 700 | 1 | |a Leoni, P. | |
| 700 | 1 | |a Leveroni, A.F. | |
| 700 | 1 | |a Recondo, E.F. | |
| 773 | 0 | |d 1971 |g v. 230 |h pp. 423-433 |k n. 3 |p Biochim. Biophys. Acta Gen. Subj. |x 03044165 |w (AR-BaUEN)CENRE-2260 |t BBA - General Subjects | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0304-4165(71)90170-X |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03044165_v230_n3_p423_Cantore |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v230_n3_p423_Cantore |y Registro en la Biblioteca Digital |
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