Leptin promotes HLA-G expression on placental trophoblasts via the MEK/Erk and PI3K signaling pathways
Introduction The development of the human haemochorial placenta requires complex regulatory mechanisms to protect invasive trophoblast cells from cytotoxic responses elicited by maternal immune cells. Leptin, the adipocyte derived hormone encoded by the Lep gene, is synthesized by placental trophobl...
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W.B. Saunders Ltd
2015
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| 024 | 7 | |2 cas |a 2 (2 amino 3 methoxyphenyl)chromone, 167869-21-8; 2 morpholino 8 phenylchromone, 154447-36-6; mitogen activated protein kinase kinase, 142805-58-1; phosphatidylinositol 3 kinase, 115926-52-8; protein kinase B, 148640-14-6; HLA-G Antigens; Leptin; Oligodeoxyribonucleotides, Antisense; Phosphatidylinositol 3-Kinase; Protein Kinase Inhibitors; Recombinant Proteins | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
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| 100 | 1 | |a Barrientos, G. | |
| 245 | 1 | 0 | |a Leptin promotes HLA-G expression on placental trophoblasts via the MEK/Erk and PI3K signaling pathways |
| 260 | |b W.B. Saunders Ltd |c 2015 | ||
| 270 | 1 | 0 | |m Blois, S.M.; Charité Center 12 Internal Medicine and Dermatology, Reproductive Medicine Research Group, Medicine UniversityGermany |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Appel, S., Turnwald, E.M., Alejandre-Alcazar, M.A., Ankerne, J., Rother, E., Janoschek, R., Leptin does not induce an inflammatory response in the murine placenta (2014) Horm Metab Res, 46 (6), pp. 384-389 | ||
| 520 | 3 | |a Introduction The development of the human haemochorial placenta requires complex regulatory mechanisms to protect invasive trophoblast cells from cytotoxic responses elicited by maternal immune cells. Leptin, the adipocyte derived hormone encoded by the Lep gene, is synthesized by placental trophoblasts and exerts pleiotropic effects on the immune system, including the promotion of inflammation and the activation of T cell responses. Methods To address its possible involvement in the modulation of maternal immune responses during pregnancy, we investigated the effect of leptin on the expression of the class Ib histocompatibility antigen HLA-G as one of the chief immunosuppressive strategies used by trophoblast cells. Results In vitro incubation of the trophoblast derived Swan 71 and JEG-3 cell lines with 25-50 ng/ml recombinant leptin significantly boosted HLA-G mRNA and protein expression, and this effect was abrogated upon pharmacological inhibition of the PI3K-Akt and MEK-Erk signaling pathways. A similar stimulatory effect of leptin was observed in term placental tissue explants, though 10-fold higher doses were required for stimulation. Further, JEG-3 cells treated with a leptin antisense oligodeoxynucleotide displayed decreased HLA-G expression levels, which were partially recovered by addition of stimulating doses of exogenous hormone. Immunofluorescence and qPCR analysis confirmed leptin biosynthesis in placental tissue, further showing that invasive extravillous trophoblast cells were a main source of this hormone during the first trimester of normal pregnancies. Discussion Taken together, our results show that leptin acts as an autocrine/paracrine signal promoting HLA-G expression in placental trophoblasts suggesting an important role in the regulation of immune evasion mechanisms at the fetal maternal interface. © 2015 Elsevier Ltd. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: Deutscher Akademischer Austausch Dienst Kairo | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Secretaría de Ciencia y Técnica, Universidad de Buenos Aires, 2010-01/2123 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: PIP 2010-247 | ||
| 536 | |a Detalles de la financiación: Fritz Thyssen Stiftung, Az. 10.10.2.125 | ||
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica, PICT 2008-0425 | ||
| 536 | |a Detalles de la financiación: The authors are especially grateful to Christine Wuillemin (Cohen's group) and Dr. I. Tirado-González (Blois's group) for their excellent technical assistance. This work was supported by grants from Universidad de Buenos Aires [UBACYT, 2010-01/2123 ], Agencia Nacional de Promoción Científica y Tecnológica [ ANPCyT PICT 2008-0425 ] and Consejo Nacional de Investigaciones Científicas y Técnicas [ CONICET PIP 2010-247 ] to C. L. V.; and the Fritz Thyssen Stiftung [Az. 10.10.2.125 ] and Charité Stiftung to S.M.B. G.B. is a postdoctoral fellow of CONICET and was granted a Reinvitation fellowship from the Deutscher Akademischer Austausch Dienst (DAAD). Appendix A | ||
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN CONICET, Buenos Aires, Argentina | ||
| 593 | |a Charité Center 12 Internal Medicine and Dermatology, Reproductive Medicine Research Group, Medicine University, Berlin, Germany | ||
| 593 | |a Laboratoire d'Hormonologie, Department of Gynaecology and Obstetrics, Geneva, Switzerland | ||
| 593 | |a Gene Therapy Laboratory, Facultad de Ciencias Biomédicas, Universidad Austral, Derqui-Buenos Aires, Argentina | ||
| 593 | |a Hospital Nacional Profesor Alejandro Posadas, Buenos Aires, Argentina | ||
| 593 | |a Departamento de Bioquímica Médica y Biología Molecular, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Sevilla, Spain | ||
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| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 650 | 1 | 7 | |2 spines |a PLACENTA |
| 700 | 1 | |a Toro, A. | |
| 700 | 1 | |a Moschansky, P. | |
| 700 | 1 | |a Cohen, M. | |
| 700 | 1 | |a Garcia, M.G. | |
| 700 | 1 | |a Rose, M. | |
| 700 | 1 | |a Maskin, B. | |
| 700 | 1 | |a Sánchez-Margalet, V. | |
| 700 | 1 | |a Blois, S.M. | |
| 700 | 1 | |a Varone, C.L. | |
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