Conjugal transfer of enterococcal transposons in Bacillus megaterium
The conjugative enterococcal transposons Tn 916 and Tn 919 were introduced into Bacillus megaterium by a filtermating technique. The transfer frequencies obtained ranged from 1.3×10-6 to 6.6×10-7. The transposons integrated stably into the B. megaterium chromosome. Tn 916 could generate auxotrophs a...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Kluwer Academic Publishers
1994
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| Sumario: | The conjugative enterococcal transposons Tn 916 and Tn 919 were introduced into Bacillus megaterium by a filtermating technique. The transfer frequencies obtained ranged from 1.3×10-6 to 6.6×10-7. The transposons integrated stably into the B. megaterium chromosome. Tn 916 could generate auxotrophs and was transferred from B. megaterium Tn 916 transconjugants to other species. © 1994 Rapid Communications of Oxford Ltd. |
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| Bibliografía: | Bertram, J., Kuhn, A., Dürre, P., Conjugal transfer and expression of streptococcal transposons in Clostridium acetobutylicum (1990) Archives of Microbiology, 153, pp. 373-377 Bertram, J., Stratz, M., Dürre, P., Natural transfer of Tn 916 between Gram-positive and Gram-negative bacteria (1991) Journal of Bacteriology, 173, pp. 443-448 Bohall, N.A., Jr, Vary, P.S., Transposition of Tn 917 in Bacillus megaterium (1986) Journal of Bacteriology, 167, pp. 716-718 Bringel, F., Van Alstine, G.L., Scott, J.R., Transfer of Tn 916 between Lactococcus lactis subsp. lactis strain is nontranspositional: Evidence for a chromosomal fertility function in strain MG1363 (1992) Journal of Bacteriology, 174, pp. 5840-5847 Burdett, V., Inamine, J., Rajagopalan, Heterogenity of tetracycline resistance determinants in Streptococcus (1982) Journal of Bacteriology, 149, pp. 995-1004 Clewell, D.B., Flannaghan, S.E., Ike, Y., Jones, J.M., Gawron-Burke, C., Sequence analysis of termini of conjugative transposon Tn 916 (1988) Journal of Bacteriology, 170, pp. 3046-3052 Clewell, D.B., Gawron-Burke, C., Conjugative transposons and the dissemination of antibiotic resistance in streptococci (1986) Annual Review of Microbiology, 40, pp. 635-665 English, J.D., Vary, P.S., Isolation of recombination defective and UV sensitive mutants of Bacillus megaterium (1986) Journal of Bacteriology, 165, pp. 155-160 Fitzgerald, G.F., Clewell, D.B., A conjugative transposon (Tn 919) in Streptococcus sanguis (1985) Infection and Immunity, 47, pp. 415-420 Fleischer, E.R., Vary, P.S., Genetic analysis of fusion recombinants and presence of non-complementing diploids in Bacillus megaterium (1985) Journal of General Microbiology, 131, pp. 919-926 Franke, A.E., Clewell, D.B., Evidence for a chromosomal borne resistance transposon (Tn 916) in Streptococcus faecalis that is capable of conjugal transfer in the absence of a conjugative plasmid (1981) Journal of Bacteriology, 145, pp. 494-502 Gawron-Burke, C., Clewell, D.B., Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn 916 in Escherichia coli: strategy for targeting and cloning of genes of Gram-positive bacteria (1984) Journal of Bacteriology, 159, pp. 214-221 Holland, J., Towner, K.J., Willia, P., Tn 916 insertional mutagenesis in Escherichia coli and Haemophilus influenzae type b following conjugative transfer (1992) Journal of General Microbiology, 138, pp. 509-515 Ivins, B.E., Welkos, S.L., Knudson, G.B., Leblanc, D.J., Transposon Tn 916 mutagenesis in Bacillus anthracis (1988) Infection and Immunity, 56, pp. 176-181 Maguin, E., Duwat, P., Hege, T., Ehrlich, D., Gruss, A., New thermosensitive plasmids for Gram-positive bacteria (1992) Journal of Bacteriology, 174, pp. 5633-5638 Maniatis, T., Frisch, E.F., Sammbrook, J., (1982) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor Laboratory, New York Millet, J., Aubert, J.P., Etude de la megatriopeptidase, protease exocellulaire de Bacillus megaterium. III. Biosynthèse et rôle physiologique (1969) Annales de l'Institut Pasteur, 117, pp. 461-473 Naglich, J.F., Andrews, R.E., Jr, Introduction of the Streptococcus faecalis transposon Tn 916 into Bacillus thuringiensis subsp. israeliensis (1988) Plasmid, 19, pp. 84-93 Norgren, M., Scott, J.R., Presence of the conjugative transposon Tn 916 in the recipient strain does not impede transfer of a second copy of the element (1991) Journal of Bacteriology, 173, pp. 319-324 Priest, F.G., Isolation and identification of aerobic endospore-forming bacteria (1989) Bacillus, pp. 293-320. , C. R., Harwood, Biotechnology Handbooks, Plenum Press, New York Saito, H., Miura, K., Preparation of transforming deoxyribonucleic acid by phenol treatment (1963) Biochimica et Biophysica Acta, 72, pp. 619-629 Scott, J.R., Kirchman, P.A., Caparon, M.C., An intermediate in transposition of the conjugative transposon Tn 916 (1988) Proceedings of the National Academy of Sciences of the United States of America, 85, pp. 4809-4813 Steinbüchel, A., Polyhydroxyalkanoic acids (1991) Biomaterials: Novel Materials from Biological Sources, pp. 123-213. , D., Byrom, Macmillan, Basingstoke, UK Vary, P.S., Transduction in Bacillus megaterium (1979) Biochemical and Biophysical Research Communications, 88, pp. 1119-1124 Vary, P.S., Development of genetic engineering in Bacillus megaterium (1992) Biology of Bacilli: Applications to Industry, pp. 251-310. , R. H., Doi, M., McGloughlin, Butterworth-Heinemann, Boston Vary, P.S., Tao, Y.P., Development of genetic methods in Bacillus megaterium (1988) Genetics and Biotechnology of Bacilli, pp. 403-407. , A. T., Ganesan, J. S., Hoch, Academic Press, New York |
| ISSN: | 09593993 |
| DOI: | 10.1007/BF00327957 |