Conjugal transfer of enterococcal transposons in Bacillus megaterium

The conjugative enterococcal transposons Tn 916 and Tn 919 were introduced into Bacillus megaterium by a filtermating technique. The transfer frequencies obtained ranged from 1.3×10-6 to 6.6×10-7. The transposons integrated stably into the B. megaterium chromosome. Tn 916 could generate auxotrophs a...

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Autor principal: Floccari, M.E
Otros Autores: Méndez, B.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Kluwer Academic Publishers 1994
Acceso en línea:Registro en Scopus
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100 1 |a Floccari, M.E. 
245 1 0 |a Conjugal transfer of enterococcal transposons in Bacillus megaterium 
260 |b Kluwer Academic Publishers  |c 1994 
270 1 0 |m Méndez, B.S.; Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina 
506 |2 openaire  |e Política editorial 
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504 |a Clewell, D.B., Gawron-Burke, C., Conjugative transposons and the dissemination of antibiotic resistance in streptococci (1986) Annual Review of Microbiology, 40, pp. 635-665 
504 |a English, J.D., Vary, P.S., Isolation of recombination defective and UV sensitive mutants of Bacillus megaterium (1986) Journal of Bacteriology, 165, pp. 155-160 
504 |a Fitzgerald, G.F., Clewell, D.B., A conjugative transposon (Tn 919) in Streptococcus sanguis (1985) Infection and Immunity, 47, pp. 415-420 
504 |a Fleischer, E.R., Vary, P.S., Genetic analysis of fusion recombinants and presence of non-complementing diploids in Bacillus megaterium (1985) Journal of General Microbiology, 131, pp. 919-926 
504 |a Franke, A.E., Clewell, D.B., Evidence for a chromosomal borne resistance transposon (Tn 916) in Streptococcus faecalis that is capable of conjugal transfer in the absence of a conjugative plasmid (1981) Journal of Bacteriology, 145, pp. 494-502 
504 |a Gawron-Burke, C., Clewell, D.B., Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn 916 in Escherichia coli: strategy for targeting and cloning of genes of Gram-positive bacteria (1984) Journal of Bacteriology, 159, pp. 214-221 
504 |a Holland, J., Towner, K.J., Willia, P., Tn 916 insertional mutagenesis in Escherichia coli and Haemophilus influenzae type b following conjugative transfer (1992) Journal of General Microbiology, 138, pp. 509-515 
504 |a Ivins, B.E., Welkos, S.L., Knudson, G.B., Leblanc, D.J., Transposon Tn 916 mutagenesis in Bacillus anthracis (1988) Infection and Immunity, 56, pp. 176-181 
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504 |a Millet, J., Aubert, J.P., Etude de la megatriopeptidase, protease exocellulaire de Bacillus megaterium. III. Biosynthèse et rôle physiologique (1969) Annales de l'Institut Pasteur, 117, pp. 461-473 
504 |a Naglich, J.F., Andrews, R.E., Jr, Introduction of the Streptococcus faecalis transposon Tn 916 into Bacillus thuringiensis subsp. israeliensis (1988) Plasmid, 19, pp. 84-93 
504 |a Norgren, M., Scott, J.R., Presence of the conjugative transposon Tn 916 in the recipient strain does not impede transfer of a second copy of the element (1991) Journal of Bacteriology, 173, pp. 319-324 
504 |a Priest, F.G., Isolation and identification of aerobic endospore-forming bacteria (1989) Bacillus, pp. 293-320. , C. R., Harwood, Biotechnology Handbooks, Plenum Press, New York 
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504 |a Scott, J.R., Kirchman, P.A., Caparon, M.C., An intermediate in transposition of the conjugative transposon Tn 916 (1988) Proceedings of the National Academy of Sciences of the United States of America, 85, pp. 4809-4813 
504 |a Steinbüchel, A., Polyhydroxyalkanoic acids (1991) Biomaterials: Novel Materials from Biological Sources, pp. 123-213. , D., Byrom, Macmillan, Basingstoke, UK 
504 |a Vary, P.S., Transduction in Bacillus megaterium (1979) Biochemical and Biophysical Research Communications, 88, pp. 1119-1124 
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504 |a Vary, P.S., Tao, Y.P., Development of genetic methods in Bacillus megaterium (1988) Genetics and Biotechnology of Bacilli, pp. 403-407. , A. T., Ganesan, J. S., Hoch, Academic Press, New York 
520 3 |a The conjugative enterococcal transposons Tn 916 and Tn 919 were introduced into Bacillus megaterium by a filtermating technique. The transfer frequencies obtained ranged from 1.3×10-6 to 6.6×10-7. The transposons integrated stably into the B. megaterium chromosome. Tn 916 could generate auxotrophs and was transferred from B. megaterium Tn 916 transconjugants to other species. © 1994 Rapid Communications of Oxford Ltd.  |l eng 
593 |a Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina 
690 1 0 |a BACILLUS MEGATERIUM 
690 1 0 |a CONJUGATIVE TRANSPOSONS 
690 1 0 |a TN 916 
690 1 0 |a TN 919 
700 1 |a Méndez, B.S. 
773 0 |d Kluwer Academic Publishers, 1994  |g v. 10  |h pp. 673-676  |k n. 6  |p World J Microbiol Biotechnol  |x 09593993  |t World Journal of Microbiology and Biotechnology 
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856 4 0 |u https://doi.org/10.1007/BF00327957  |y DOI 
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