Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract

Rhodopseudomonas viridis was grown in the presence and in the absence of yeast extract. The cells grown under this latter condition present a ten fold diminished bacteriochlorophyll (bchl) content. This decrease was paralleled by a similar increase in the ratio lipid phosphorous/bchl, whereas the or...

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Autor principal: Pucheu, N.L
Otros Autores: Kerber, N.L, García, A.F
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Springer-Verlag 1974
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-0016262110 
024 7 |2 cas  |a lipid, 66455-18-3; ornithine, 70-26-8, 7006-33-9; protein, 67254-75-5; Bacterial Proteins; Chlorophyll, 1406-65-1; Culture Media; Fatty Acids; Lipids; Ornithine, 7006-33-9; Phosphorus, 7723-14-0 
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030 |a AMICC 
100 1 |a Pucheu, N.L. 
245 1 0 |a Comparative studies on membranes isolated from Rhodopseudomonas viridis grown in the presence and in the absence of yeast extract 
260 |b Springer-Verlag  |c 1974 
270 1 0 |m Pucheu, N.L.; Centro de Investigaciones Microbiológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Bartlett, G.R., Phosphorous assay in column chromatography (1959) J. biol. Chem., 234, pp. 466-468 
504 |a Blight, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Canadian Journal of Biochemistry and Physiology, 37, pp. 911-917 
504 |a Bregoff, H.M., Roberts, E., Delwiche, C.C., Paper chromatography of quaternary ammonium bases and related compounds (1953) J. biol. Chem., 205, pp. 565-574 
504 |a Chinard, F.P., Photometric estimations of proline and ornithine (1952) J. biol. Chem., 199, pp. 91-95 
504 |a Cohen-Bazire, G., Kunisawa, R., Some observations on the synthesis and function of the photosynthetic apparatus in Rhodospirillum rubrum (1960) Proceedings of the National Academy of Sciences, 46, pp. 1543-1553 
504 |a Cohen-Bazire, G., Sistrom, W.R., Stanier, R.Y., Kinetic studies of pigment synthesis by non-sulphur bacteria (1957) J. cell. comp. Physiol., 49, pp. 25-68 
504 |a Drews, G., Giesbrecht, P., Zur Morphogenese der Bakterien-“Chromatophoren” (=Thylakoide) und zur Synthese des Bakteriochlorophylls bei Rhodopseudomonas spheroides und Rhodospirillum rubrum (1963) Zbl. Bakt., I. Abt. Orig., 190, pp. 508-536 
504 |a Folch, J., Lees, M., sloane-Stanley, G.H.S., A simple method for the isolation and purification of total lipides from animal tissues (1957) J. biol. Chem., 226, pp. 497-509 
504 |a Fraker, P.J., Kaplan, S., Isolation and fractionation of the photosynthetic membranous organelles from Rhodopseudomonas spheroides (1971) J. Bact., 108, pp. 465-473 
504 |a Fraker, P.J., Kaplan, S., Isolation and characterization of a bacteriochlorophyll-containing protein from Rhodopseudomonas spheroides (1972) J. biol. Chem., 247, pp. 2732-2737 
504 |a Giesbrecht, P., Drews, G., Elektronenmikroskopische Untersuchungen über die Entwicklung der “Chromatophoren” von Rhodospirillum molischianum Giesberger (1962) Arch. Mikrobiol., 43, pp. 152-161 
504 |a Holt, S.C., Marr, A.G., Isolation and purification of the intracytoplasmic membranes of Rhodospirillum rubrum (1965) J. Bact., 89, pp. 1513-1520 
504 |a Loach, P.A., Hadsell, R.M., Sekura, D.L., Stemer, A., Quantitative dissolution of the membrane and preparation of photoreceptor subunits from Rhodospirillum rubrum (1970) Biochemistry, 8, pp. 3127-3135 
504 |a Loach, P.A., Sekura, D.L., Hadsell, R.M., Stemer, A., Quantitative dissolution of the membrane and preparation of photoreceptor subunits from Rhodopseudomonas spheroides (1970) Biochemistry, 9, pp. 724-733 
504 |a Lowry, O.H., Rosebrough, N.H., Farr, A.L., Randall, R.J., Protein measurement with the Folin-phenol reagent (1951) J. biol. Chem., 193, pp. 265-275 
504 |a Nichols, B.W., (1964) New biochemical separation, pp. 321-337. , A. T., James, L. J., Morris, Van Nostrand, London 
504 |a Oelze, J., Drews, G., Membranes of photosynthetic bacteria (1972) Biochim. biophys. Acta (Amst.), 265, pp. 209-239 
504 |a Pucheu, N.L., Kerber, N.L., García, A.F., Some environmental factors influencing the state of the membranes isolated by gradient centrifugation from cell free extracts of Rhodopseudomonas viridis (1973) FEBS Letters, 33, pp. 119-124 
504 |a Schröder, J., Biedermann, M., Drews, G., Die Fettsäuren in ganzen Zellen, Thylakoiden und Lipopolysacchariden von Rhodospirillum rubrum und Rhodopseudomonas capsulata (1969) Arch. Mikrobiol., 66, pp. 273-280 
504 |a Schröder, J., Drews, G., Quantitative Bestimmung der Fettsäuren von Rhodospirillum rubrum und Rhodopseudomonas capsulata während der Thylakoidmorphogenese (1968) Arch. Mikrobiol., 64, pp. 59-70 
504 |a Tauschel, H.D., Drews, G., Thylakoidmorphogenese bei Rhodopseudomonas palustris (1967) Arch. Mikrobiol., 59, pp. 381-404 
504 |a Thornber, J.P., Olson, J.M., Willia, D.M., Clayton, M.J., Isolation of the reaction center of Rhodopseudomonas viridis (1969) Biochimica et Biophysica Acta (BBA) - Bioenergetics, 172, pp. 351-354 
504 |a Vaskowsky, V.E., Kotsketsky, E.Y., Modified spray for the detection of phospholipids on thin layer chromatogramms (1968) J. Lipid Res., 9, p. 396 
504 |a Weber, K., Osborn, M., The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis (1969) J. biol. Chem., 244, pp. 4406-4412 
520 3 |a Rhodopseudomonas viridis was grown in the presence and in the absence of yeast extract. The cells grown under this latter condition present a ten fold diminished bacteriochlorophyll (bchl) content. This decrease was paralleled by a similar increase in the ratio lipid phosphorous/bchl, whereas the ornithine lipid/bchl ratio remains constant. Some quantitative differences in the fatty acid composition are also reported. The protein composition of both membranes was also studied, only indicating quantitative differences. An active reaction center preparation was obtained from both types of cells. When isolated from cells grown in the presence of yeast extract, this reaction center preparation shows the presence of proteins a, b, c and d. Further treatment of this active reaction center results, in cells grown under either condition, in the isolation of green (oxidized behl) and brown (inactive reaction center) bchl containing fractions. The protein composition and absorption spectrum of the inactive reaction centers obtained from both types of cells were identical (proteins a, c and d). On the other hand the green complexes differ in their protein composition as well as in their absorption spectrum. © 1974 Springer-Verlag.  |l eng 
593 |a Centro de Investigaciones Microbiológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
690 1 0 |a LIPID AND PROTEIN COMPOSITION 
690 1 0 |a PHOTOSYNTHETIC MEMBRANE 
690 1 0 |a REACTION CENTER 
690 1 0 |a BACTERIOCHLOROPHYLL 
690 1 0 |a LIPID 
690 1 0 |a ORNITHINE 
690 1 0 |a PROTEIN 
690 1 0 |a BACTERIAL CELL WALL 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a MICROORGANISM 
690 1 0 |a RHODOPSEUDOMONAS VIRIDIS 
690 1 0 |a THEORETICAL STUDY 
690 1 0 |a BACTERIAL PROTEINS 
690 1 0 |a CELL FRACTIONATION 
690 1 0 |a CELL MEMBRANE 
690 1 0 |a CHLOROPHYLL 
690 1 0 |a CHROMATOGRAPHY, THIN LAYER 
690 1 0 |a COMPARATIVE STUDY 
690 1 0 |a CULTURE MEDIA 
690 1 0 |a ELECTROPHORESIS 
690 1 0 |a FATTY ACIDS 
690 1 0 |a LIPIDS 
690 1 0 |a ORNITHINE 
690 1 0 |a PHOSPHORUS 
690 1 0 |a RHODOPSEUDOMONAS 
690 1 0 |a SACCHAROMYCES CEREVISIAE 
700 1 |a Kerber, N.L. 
700 1 |a García, A.F. 
773 0 |d Springer-Verlag, 1974  |g v. 101  |h pp. 259-272  |k n. 1  |p Arch. Microbiol.  |x 03028933  |t Archives of Microbiology 
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