In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii
Yeast cells of the dimorphic fungus Mucor rouxii have been permeabilized by treatment with toluene:ethanol. The permeabilization allowed the in situ measurement of pyruvate kinase, cAMP phosphodiesterase and adenylate cyclase activities. Using a small peptide as substrate, cAMP dependent protein kin...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1982
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 08565caa a22009137a 4500 | ||
|---|---|---|---|
| 001 | PAPER-1231 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203034.0 | ||
| 008 | 190411s1982 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0020349508 | |
| 024 | 7 | |2 cas |a 3',5' cyclic nucleotide phosphodiesterase, 9040-59-9; adenylate cyclase, 9012-42-4; cyclic AMP, 60-92-4; protein kinase, 9026-43-1; pyruvate kinase, 9001-59-6; toluene, 108-88-3; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Adenylate Cyclase, EC 4.6.1.1; Cyclic AMP, 60-92-4; Protein Kinases, EC 2.7.1.37; Pyruvate Kinase, EC 2.7.1.40; Toluene, 108-88-3 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a CBRPD | ||
| 100 | 1 | |a Maggese, M.C. | |
| 245 | 1 | 3 | |a In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii |
| 260 | |c 1982 | ||
| 270 | 1 | 0 | |m Passeron, S.; Career Investigator of the Consejo Nacional de Investigaciones Científicas y TécnicasArgentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Bartnicki-García, Nickerson, Nutrition, growth and morphogenesis of Mucor rouxii (1962) Journal of Bacteriology, 84, pp. 841-858 | ||
| 504 | |a Bartnicki-García, Cell wall chemistry, morphogenesis, and taxonomy of fungi (1968) Annual Review of Microbiology, 22, pp. 87-108 | ||
| 504 | |a Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Analytical Biochemistry, 72, pp. 248-254 | ||
| 504 | |a Cantore, Galvagno, Passeron, Variations in the levels of cyclic adenosine 3′–5′ monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3′–5′ monophosphate phosphodiesterase during aerobic morphogenesis of Mucor rouxii (1980) Archives of Biochemistry and Biophysics, 199, pp. 312-320 | ||
| 504 | |a Cantore, Passeron, Kinetic properties solubilization and molecular characterization of Mucor rouxii adenylate cyclase (1982) Arch Biochem Biophys, , in press | ||
| 504 | |a Felix, Permeabilized cells (1982) Analytical Biochemistry, 120, pp. 211-234 | ||
| 504 | |a Galvagno, Moreno, Cantore, Passeron, Cyclic adenosine 3′–5′ monophosphate phosphodiesterase from Mucor rouxii: regulation of enzyme activity by phosphorylation and dephosphorylation (1979) Biochemical and Biophysical Research Communications, 89, pp. 779-785 | ||
| 504 | |a Glass, Masaracchia, Feramisco, Kemp, Isolation of phosphorylated peptides and proteins on ionexchange papers (1978) Analytical Biochemistry, 87, pp. 566-575 | ||
| 504 | |a Kemp, Clark, Adrenergic control of the cyclic AMP-dependent protein kinase and pyruvate kinase in isolated hepatocytes (1978) Journal of Biological Chemistry, 253, pp. 5147-5154 | ||
| 504 | |a Larsen, Sypherd, Cyclic adenosine 3′,5′-monophosphate and morphogenesis in Mucor racemosus (1974) Journal of Bacteriology, 117, pp. 432-438 | ||
| 504 | |a Leloir, Goldenberg, Synthesis of glycogen from uridine diphosphate glucose in liver (1960) Journal of Biological Chemistry, 235, pp. 919-923 | ||
| 504 | |a Moreno, Galvagno, Passeron, Control of Mucor rouxii adenosine 3′–5′ monophosphate phosphodiesterase by phosphorylation-dephosphorylation and proteolysis (1982) Archives of Biochemistry and Biophysics, 214, pp. 573-580 | ||
| 504 | |a Moreno, Passeron, Further studies on cyclic adenosine 3′–5′ monophosphate protein kinase from the dimorphic fungus Mucor rouxii (1980) Archives of Biochemistry and Biophysics, 199, pp. 321-330 | ||
| 504 | |a Moreno, Paveto, Passeron, Multiple protein kinase activities in the dimorphic fungus Mucor rouxii. Comparison with a cyclic adenosine 3′–5′-monophosphate binding protein (1977) Archives of Biochemistry and Biophysics, 180, pp. 225-231 | ||
| 504 | |a Pastori, Kerner, Moreno, Passeron, cAMP-dependent protein kinase from Mucor rouxii: physical evidence of a ternary complex holoenzyme-cAMP (1981) Biochemical and Biophysical Research Communications, 101, pp. 663-671 | ||
| 504 | |a Paveto, Epstein, Passeron, Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii (1975) Archives of Biochemistry and Biophysics, 169, pp. 449-456 | ||
| 504 | |a Paveto, Passeron, Some kinetic properties of Mucor rouxii phosphofructokinase. Effect of cyclic adenosine 3′–5′ monophosphate (1977) Archives of Biochemistry and Biophysics, 178, pp. 1-7 | ||
| 504 | |a Reeves, Sols, Regulation of Escherichia coli phosphofructokinase in situ (1973) Biochemical and Biophysical Research Communications, 50, pp. 459-466 | ||
| 504 | |a Salomon, Londos, Rodbell, A highly sensitive adenylate cyclase assay (1974) Analytical Biochemistry, 58, pp. 541-548 | ||
| 504 | |a Serrano, Gancedo, Gancedo, Assay of yeast enzymes in vitro (1973) European Journal of Biochemistry, 34, pp. 479-482 | ||
| 504 | |a Sols, Marco, Concentrations of Metabolites and Binding Sites Implications in Metabolic Regulation (1970) Current Topics in Cellular Regulation, 2, pp. 227-273. , B.L. Horecker, E.R. Stadtman, Academic Press, New York | ||
| 504 | |a Srere, Enzymes concentrations in tissues (1967) Science, 158, pp. 936-937 | ||
| 504 | |a Sypherd, Borgia, Paznokas, Biochemistry of dimorphism in the fungus Mucor (1978) Advances in Microbiological Physiology, 18, pp. 67-104 | ||
| 504 | |a Terenzi, Rosellino, Passeron, Two types of pyruvate kinase in Mucor rouxii. Changes in the enzymatic pattern related to aerobic development (1971) European Journal of Biochemistry, 18, pp. 342-350 | ||
| 520 | 3 | |a Yeast cells of the dimorphic fungus Mucor rouxii have been permeabilized by treatment with toluene:ethanol. The permeabilization allowed the in situ measurement of pyruvate kinase, cAMP phosphodiesterase and adenylate cyclase activities. Using a small peptide as substrate, cAMP dependent protein kinase activity could also be measured. Permeabilized cells showed higher cAMP phosphodiesterase and adenylate cyclase activities than cellular homogenates. The main catalytic properties of the enzymes were similar to that previously found in in vitro studies. © 1982. |l eng | |
| 536 | |a Detalles de la financiación: Consejo De Ciencia, Innovación Y Tecnología Del Estado De Yucatán | ||
| 536 | |a Detalles de la financiación: ACKNOWLEDGEMENTSW. e thank Dr. Silvia Moreno for her constructive criticism of the manuscript. This work was supported by grants from the "Consejo National de Investiuaciones Cientificas y Tecnicas", 'Subsecretaria de Estado de Ciencia y Tecnologia" and "Comision Na-cional de Energia Atomica". | ||
| 593 | |a Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica y Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina | ||
| 593 | |a Career Investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina | ||
| 690 | 1 | 0 | |a 3',5' CYCLIC NUCLEOTIDE PHOSPHODIESTERASE |
| 690 | 1 | 0 | |a ADENYLATE CYCLASE |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a PROTEIN KINASE |
| 690 | 1 | 0 | |a PYRUVATE KINASE |
| 690 | 1 | 0 | |a TOLUENE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MUCOR |
| 690 | 1 | 0 | |a PERMEABILITY |
| 690 | 1 | 0 | |a 3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE |
| 690 | 1 | 0 | |a ADENYLATE CYCLASE |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a MUCOR |
| 690 | 1 | 0 | |a PERMEABILITY |
| 690 | 1 | 0 | |a PROTEIN KINASES |
| 690 | 1 | 0 | |a PYRUVATE KINASE |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TOLUENE |
| 700 | 1 | |a Galvagno, M.A. | |
| 700 | 1 | |a Cantore, M.L. | |
| 700 | 1 | |a Passeron, S. | |
| 773 | 0 | |d 1982 |g v. 6 |h pp. 1101-1108 |k n. 12 |p Cell Biol. Int. Rep. |x 03091651 |t Cell Biology International Reports | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0309-1651(82)90027-3 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03091651_v6_n12_p1101_Maggese |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v6_n12_p1101_Maggese |y Registro en la Biblioteca Digital |
| 961 | |a paper_03091651_v6_n12_p1101_Maggese |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 62184 | ||