From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments

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Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusi...

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Autor principal: Ipiña, E.P
Otros Autores: Dawson, S.P
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2013
Acceso en línea:Registro en Scopus
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Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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100 1 |a Ipiña, E.P. 
245 1 5 |a From free to effective diffusion coefficients in fluorescence correlation spectroscopy experiments 
260 |c 2013 
270 1 0 |m Ipiña, E.P.; Departamento de Física, FCEN-UBA, Pabellón i, 1428 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
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520 3 |a Diffusion is one of the main transport processes that occur inside cells determining the spatial and time distribution of relevant action molecules. In most cases these molecules not only diffuse but also interact with others as they get transported. When these interactions occur faster than diffusion the resulting transport can be characterized by "effective diffusion coefficients" that depend on both the reaction rates and the "free" diffusion coefficients. Fluorescence correlation spectroscopy (FCS) gives information on effective rather than free diffusion coefficients under this condition. In the present paper we investigate what coefficients can be drawn from FCS experiments for a wide range of values of the ratio of reaction to diffusion time scales, using different fitting functions. We find that the effective coefficients can be inferred with relatively small errors even when the condition of fast reactions does not exactly hold. Since the diffusion time scale depends on the size of the observation volume and the reaction time scale depends on concentrations, we also discuss how by changing either one or the other property one can switch between the two limits and extract more information on the system under study. © 2013 American Physical Society.  |l eng 
593 |a Departamento de Física, FCEN-UBA, Pabellón i, 1428 Buenos Aires, Argentina 
690 1 0 |a DIFFUSION TIME 
690 1 0 |a EFFECTIVE DIFFUSION COEFFICIENTS 
690 1 0 |a FAST REACTION 
690 1 0 |a FITTING FUNCTIONS 
690 1 0 |a FLUORESCENCE CORRELATION SPECTROSCOPY 
690 1 0 |a OTHER PROPERTIES 
690 1 0 |a TIME DISTRIBUTION 
690 1 0 |a TRANSPORT PROCESS 
690 1 0 |a EXPERIMENTS 
690 1 0 |a FLUORESCENCE SPECTROSCOPY 
690 1 0 |a MOLECULES 
690 1 0 |a REACTION RATES 
690 1 0 |a TIME MEASUREMENT 
690 1 0 |a DIFFUSION 
690 1 0 |a BIOPOLYMER 
690 1 0 |a ARTICLE 
690 1 0 |a BIOLOGICAL MODEL 
690 1 0 |a CHEMICAL MODEL 
690 1 0 |a CHEMISTRY 
690 1 0 |a COMPUTER SIMULATION 
690 1 0 |a DIFFUSION 
690 1 0 |a METABOLISM 
690 1 0 |a METHODOLOGY 
690 1 0 |a SPECTROFLUOROMETRY 
690 1 0 |a BIOPOLYMERS 
690 1 0 |a COMPUTER SIMULATION 
690 1 0 |a DIFFUSION 
690 1 0 |a MODELS, BIOLOGICAL 
690 1 0 |a MODELS, CHEMICAL 
690 1 0 |a SPECTROMETRY, FLUORESCENCE 
700 1 |a Dawson, S.P. 
773 0 |d 2013  |g v. 87  |k n. 2  |p Phys. Rev. E Stat. Nonlinear Soft Matter Phys.  |x 15393755  |t Physical Review E - Statistical, Nonlinear, and Soft Matter Physics 
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