Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco

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The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populatio...

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Autor principal: Piccinali, R.V
Otros Autores: Marcet, P.L, Ceballos, L.A, Kitron, U., Gürtler, Ricardo Esteban, Dotson, E.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2011
Materias:
ARGENTINA
Acceso en línea:Registro en Scopus
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Piccinali, R.V. 
245 1 0 |a Genetic variability, phylogenetic relationships and gene flow in Triatoma infestans dark morphs from the Argentinean Chaco 
260 |c 2011 
270 1 0 |m Piccinali, R.V.; Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2do piso, Pabellón 2, Ciudad Universitaria, Int. Güiraldes 2160, C1429EGA, Ciudad Autónoma de Buenos Aires, Argentina; email: rpicci@ege.fcen.uba.ar 
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506 |2 openaire  |e Política editorial 
520 3 |a The recent discovery of sylvatic populations of Triatoma infestans outside the Andean Valleys of Bolivia prompted an evolutionary question about the putative ancestral area of origin and dispersal of the species, and an epidemiological question regarding the possible role of these sylvatic populations in the recolonization process of insecticide-treated houses. The finding of a population of sylvatic melanic T. infestans (dark morphs) in the Argentinean dry Chaco at 7. km from a peridomestic bug population of typical coloration gave us the opportunity to test both questions simultaneously by employing phylogenetic and population genetic approaches. For this purpose we analyzed sylvatic and peridomestic bugs using sequence-based mitochondrial and nuclear markers (mtCOI and ITS-1) and microsatellites. Sylvatic bugs were confirmed to be T. infestans and not hybrids, and showed high levels of genetic variability and departures from neutral expectations for mtCOI variation. New ITS-1 and mtCOI haplotypes were recorded, as well as haplotypes shared with peridomestic and/or domestic bugs from previous records. The peridomestic population was invariant for ITS-1 and mtCOI, but showed variability for microsatellites and signatures of a population bottleneck, probably due to a limited number of founders. Phylogenetic analyses were consistent with the presence of ancestral haplotypes in sylvatic bugs. According to F-statistics and assignment methods there was a significant differentiation between sylvatic and peridomestic bugs and gene flow was low and asymmetric, with more bugs moving from the peridomicile to the sylvatic environment. These results support the hypothesis of the Chaco region as the area of origin of T. infestans, and a limited role of sylvatic melanic T. infestans in peridomestic infestation in the Argentinean Chaco. © 2011 Elsevier B.V.  |l eng 
536 |a Detalles de la financiación: Fogarty International Center 
536 |a Detalles de la financiación: Cornell University 
536 |a Detalles de la financiación: National Institute of Environmental Health Sciences 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica 
536 |a Detalles de la financiación: National Health Research Institutes, 01 TW05836 
536 |a Detalles de la financiación: LAMARC 2.1.3, MRBAYES 3.1 and part of the STRUCTURE 2.3.3 runs were carried out by using the resources of the Computational Biology Service Unit from Cornell University which is partially funded by Microsoft Corporation. Many thanks to the CDC core facilities for providing the microsatellite and mtCO1 oligonucleotides. M.S. Rodriguero and J.M. Gurevitz are greatly acknowledged for many helpful discussions and ideas that improved this manuscript. This work was founded by grants from National Institutes of Health Research Grant #R01 TW05836 funded by the Fogarty International Center and the National Institute of Environmental Health Sciences (U.K. and R.E.G.), TDR/WHO , Universidad de Buenos Aires and Agencia Nacional de Promoción Científica y Técnica (R.E.G). R.V.P. and R.E.G. are members of CONICET Carrera de Investigador Científico (Argentina). The findings and conclusions in this manuscript are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention. 
593 |a Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina 
593 |a Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, Entomology Branch, Atlanta, GA, United States 
593 |a Department of Environmental Studies, Emory University, Atlanta, GA, United States 
651 4 |a ARGENTINA 
690 1 0 |a DARK MORPHS 
690 1 0 |a EPIDEMIOLOGICAL RELEVANCE 
690 1 0 |a EVOLUTIONARY HISTORY 
690 1 0 |a GENE FLOW 
690 1 0 |a TRIATOMA INFESTANS 
690 1 0 |a MITOCHONDRIAL PROTEIN 
690 1 0 |a NUCLEAR PROTEIN 
690 1 0 |a PROTEIN ITS 1 
690 1 0 |a PROTEIN MTCOI 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ARTICLE 
690 1 0 |a BOTTLENECK POPULATION 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DOMESTIC SPECIES 
690 1 0 |a ENVIRONMENTAL FACTOR 
690 1 0 |a GENE 
690 1 0 |a GENE FLOW 
690 1 0 |a GENE SEQUENCE 
690 1 0 |a GENETIC VARIABILITY 
690 1 0 |a HAPLOTYPE 
690 1 0 |a INSECT GENETICS 
690 1 0 |a ITS 1 GENE 
690 1 0 |a MICROSATELLITE MARKER 
690 1 0 |a MTCOI GENE 
690 1 0 |a NONHUMAN 
690 1 0 |a NUCLEOTIDE SEQUENCE 
690 1 0 |a PHYLOGENY 
690 1 0 |a POPULATION GENETICS 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a TRIATOMA INFESTANS 
690 1 0 |a ANIMALS 
690 1 0 |a BASE SEQUENCE 
690 1 0 |a DNA, INTERGENIC 
690 1 0 |a ELECTRON TRANSPORT COMPLEX IV 
690 1 0 |a GENE EXPRESSION REGULATION 
690 1 0 |a GENE FLOW 
690 1 0 |a GENETIC VARIATION 
690 1 0 |a HAPLOTYPES 
690 1 0 |a MITOCHONDRIA 
690 1 0 |a PHYLOGENY 
690 1 0 |a PIGMENTS, BIOLOGICAL 
690 1 0 |a TRIATOMA 
690 1 0 |a TRIATOMA INFESTANS 
700 1 |a Marcet, P.L. 
700 1 |a Ceballos, L.A. 
700 1 |a Kitron, U. 
700 1 |a Gürtler, Ricardo Esteban 
700 1 |a Dotson, E.M. 
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