The cytosolic invertase NI6 affects vegetative growth, flowering, fruit set, and yield in tomato

Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble and insoluble invertases have been largely inves...

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Otros Autores: Coluccio Leskow, Carla, Conte, Mariana, Del Pozo, Talia, Bermúdez, Luisa, Silvestre Lira, Bruno, Gramegna, Giovanna, Baroli, Irene, Carrari, Fernando
Formato: Artículo
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Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2021coluccioleskow.pdf
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245 1 0 |a The cytosolic invertase NI6 affects vegetative growth, flowering, fruit set, and yield in tomato 
520 |a Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble and insoluble invertases have been largely investigated, studies on the role of neutral invertases (A/N-INV) have lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with a previously reported quantitative trait locus (QTL) largely affecting primary carbon metabolism in tomato. Of the eight A/N-INV genes identified in the tomato genome, NI6 mRNA is present in all organs, but its expression was higher in sink tissues (mainly roots and fruits). A NI6-GFP fusion protein localized to the cytosol of mesophyll cells. Tomato NI6- silenced plants showed impaired growth phenotype, delayed flowering and a dramatic reduction in fruit set. Global gene expression and metabolite profile analyses of these plants revealed that NI6 is not only essential for sugar metabolism, but also plays a signaling role in stress adaptation. We also identified major hubs, whose expression patterns were greatly affected by NI6 silencing; these hubs were within the signaling cascade that coordinates carbohydrate metabolism with growth and development in tomato. 
650 |2 Agrovoc  |9 26 
653 |a CARBON PARTITIONING 
653 |a CYTOSOLIC INVERTASE 
653 |a SIGNALING 
653 |a SUCROSE METABOLISM 
653 |a TOMATO 
700 1 |a Coluccio Leskow, Carla  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Buenos Aires, Argentina.  |u CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Argentina.  |9 73980 
700 1 |a Conte, Mariana  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Buenos Aires, Argentina.  |u CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Argentina.  |9 70031 
700 1 |a Del Pozo, Talia  |u Universidad Mayor. Escuela de Agronomía. Centro Tecnológico de Recursos Vegetales. Huechuraba, Santiago, Chile.  |9 73981 
700 1 |a Bermúdez, Luisa  |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Buenos Aires, Argentina.  |u CONICE - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto De Investigación Biotecnología (IB). Hurlingham, Argentina.  |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética. Buenos Aires, Argentina.  |9 68277 
700 1 |a Silvestre Lira, Bruno  |u Universidade de São Paulo. Instituto de Biociências. Departamento de Botânica. São Paulo, SP, Brazil.  |9 73982 
700 1 |a Gramegna, Giovanna  |u Universidade de São Paulo. Departamento de Botânica. Instituto de Biociências. São Paulo, SP, Brazil.  |9 73983 
700 1 |a Baroli, Irene  |u Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA). Buenos Aires, Argentina.  |u CONICET - Instituto de Biodiversidad y Biología Experimental Aplicada (IBBEA). Buenos Aires, Argentina.  |9 73984 
700 1 |a Carrari, Fernando  |u Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética. Buenos Aires, Argentina.  |u Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, CONICET-UBA). Buenos Aires, Argentina.  |u CONICET - Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, CONICET-UBA). Buenos Aires, Argentina.  |9 11158 
773 0 |t Journal of experimental botany  |w (AR-BaUFA)SECS000114  |g Vol.72, no.7 (2021), p.2525-2543, grafs., tbls., fot. 
856 |f 2021coluccioleskow  |i en internet  |q application/pdf  |u http://ri.agro.uba.ar/files/download/articulo/2021coluccioleskow.pdf  |x ARTI202206 
856 |u https://academic.oup.com  |z LINK AL EDITOR 
942 |c ARTICULO 
942 |c ENLINEA 
976 |a AAG