Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus

The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0-200 ?L and hemicellulase amounts of 0-200 mg for 5 g of material, we explor...

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Otros Autores: Santo Domingo, Cinthia, Soria, Marcelo Abel, Rojas, Ana M., Fissore, Eliana N., Gerschenson, Lia N.
Formato: Artículo
Lenguaje:Español
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Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2015santodomingo.pdf
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024 |a 10.3390/ijms16036057 
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245 1 0 |a Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus 
520 |a The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0-200 ?L and hemicellulase amounts of 0-200 mg for 5 g of material, we explored the effect of a 5 h enzymatic treatment at 40 °C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol. 
650 |2 Agrovoc  |9 26 
653 0 |a URONIC ACID 
653 0 |a SPECTROPHOTOMETRY 
653 0 |a PROTEINASE 
653 0 |a PROTEIN ANALYSIS 
653 0 |a PROTEIN 
653 0 |a PROTEASE 
653 0 |a POLYPHENOLS 
653 0 |a POLYPHENOL 
653 0 |a PECTIN 
653 0 |a NONHUMAN 
653 0 |a INULIN 
653 0 |a HYDROLYSIS 
653 0 |a HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 
653 0 |a HEMICELLULOSE 
653 0 |a HEMICELLULASE 
653 0 |a GALACTURONIC ACID 
653 0 |a FLOW RATE 
653 0 |a ENZYME ISOLATION 
653 0 |a ENZYME ACTIVITY 
653 0 |a ELECTROSPRAY MASS SPECTROMETRY 
653 0 |a DIETARY FIBER 
653 0 |a CYNARA CARDUNCULUS 
653 0 |a CONTROLLED STUDY 
653 0 |a CHEMICAL COMPOSITION 
653 0 |a CARDOON 
653 0 |a BIOMASS TO CHEMICALS 
700 1 |a Santo Domingo, Cinthia  |9 71589 
700 1 |9 49057  |a Soria, Marcelo Abel 
700 1 |a Rojas, Ana M.  |9 70153 
700 1 |a Fissore, Eliana N.  |9 68705 
700 1 |a Gerschenson, Lia N.  |9 67234 
773 |t International Journal of Molecular Sciences  |g vol.16, no.3 (2015), p.6057- 6075 
856 |u http://ri.agro.uba.ar/files/download/articulo/2015santodomingo.pdf  |i En internet  |q application/pdf  |f 2015santodomingo  |x MIGRADOS2018 
856 |u http://www.mdpi.com/journal/ijms  |x MIGRADOS2018  |z LINK AL EDITOR 
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