Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation

Antioxidant enzymes play a key role in plant tolerance to different types of stress, including ultraviolet-B [UV-B] radiation. Here we report that nitric oxide [NO] enhances antioxidant enzymes gene expression and increases the activity of specific isoforms protecting against UV-B radiation. Pre-tre...

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Otros Autores: Santa Cruz, Diego Mario, Pacienza, Natalia, Zilli, Carla G., Tomaro, María L., Balestrasse, Karina Beatriz, Yannarelli, Gustavo G.
Formato: Artículo
Lenguaje:Español
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Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2014santacruz.pdf
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Aporte de:Registro referencial: Solicitar el recurso aquí
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024 |a 10.1016/j.jphotobiol.2014.09.019 
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245 1 0 |a Nitric oxide induces specific isoforms of antioxidant enzymes in soybean leaves subjected to enhanced ultraviolet - B radiation 
520 |a Antioxidant enzymes play a key role in plant tolerance to different types of stress, including ultraviolet-B [UV-B] radiation. Here we report that nitric oxide [NO] enhances antioxidant enzymes gene expression and increases the activity of specific isoforms protecting against UV-B radiation. Pre-treatments with sodium nitroprussiate [SNP], a NO-donor, prevented lipid peroxidation, ion leakage and H2O2 and superoxide anion accumulation in leaves of UV-B-treated soybean plants. Transcripts levels of superoxide dismutase [SOD], catalase [CAT] and ascorbate peroxidase [APX] were significantly induced by SNP. These data correlated with the enhancement of particular antioxidant enzyme isoforms, such as one CAT isoform and two APX isoforms. Moreover, SNP induced the expression of three new isoforms of SOD, identified as Mn-SOD subclass. Further results showed that total activities of SOD, CAT and APX significantly increased by 2.2-, 1.8- and 2.1-fold in SNP-treated plants compared to controls, respectively. The protective effect of SNP against UV-B radiation was negated by addition of the specific NO scavenger cPTIO, indicating that NO released by SNP mediates the enhancement of antioxidant enzymes activities. In conclusion, NO is involved in the signaling pathway that up-regulates specific isoforms of antioxidant enzymes protecting against UV-B-induced oxidative stress. 
653 0 |a ASCORBATE PEROXIDASE 
653 0 |a CATALASE 
653 0 |a CONTROLLED STUDY 
653 0 |a ENZYME ACTIVATION 
653 0 |a ENZYME ACTIVITY 
653 0 |a ENZYME INDUCTION 
653 0 |a HYDROGEN PEROXIDE 
653 0 |a ION 
653 0 |a ION TRANSPORT 
653 0 |a LIPID 
653 0 |a LIPID PEROXIDATION 
653 0 |a MANGANESE SUPEROXIDE DISMUTASE 
653 0 |a NITRIC OXIDE 
653 0 |a NITROPRUSSIDE SODIUM 
653 0 |a NONHUMAN 
653 0 |a PEROXIDASE 
653 0 |a PLANT DEFENSE 
653 0 |a PLANT LEAF 
653 0 |a RADIATION PROTECTION 
653 0 |a SOYBEAN 
653 0 |a SUPEROXIDE 
653 0 |a SUPEROXIDE DISMUTASE 
653 0 |a ULTRAVIOLET B RADIATION 
700 1 |9 48329  |a Santa Cruz, Diego Mario 
700 1 |a Pacienza, Natalia  |9 49509 
700 1 |9 48328  |a Zilli, Carla G. 
700 1 |a Tomaro, María L.  |9 48332 
700 1 |a Balestrasse, Karina Beatriz  |9 37343 
700 1 |9 48331  |a Yannarelli, Gustavo G. 
773 |t Journal of Photochemistry and Photobiology B: Biology  |g vol.141 (2014), p.202-209 
856 |u http://ri.agro.uba.ar/files/intranet/articulo/2014santacruz.pdf  |i En reservorio  |q application/pdf  |f 2014santacruz  |x MIGRADOS2018 
856 |u http://www.elsevier.com/  |x MIGRADOS2018  |z LINK AL EDITOR 
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