Reduced faecal shedding of Escherichia coli O157 H7 in cattle following systemic vaccination with Y - intimin C 280 and EspB proteins

Enterohaemorrhagic Escherichia coli [EHEC] O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome [HUS] worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This stud...

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Detalles Bibliográficos
Otros Autores: Vilte, Daniel A., Larzábal, Mariano, Garbaccio, Sergio, Gammella, Mariela, Rabinovitz, Bettina C., Elizondo, Ana M., Cantet, Rodolfo Juan Carlos, Delgado, Fernando, Meikle, Virginia, Cataldi, Angel Adrián, Mercado, Elsa C.
Formato: Artículo
Lenguaje:Español
Materias:
E. COLI O157:H7
ESPB
INTIMIN
VACCINE
ESCHERICHIA COLI PROTEIN
IMMUNOGLOBULIN A ANTIBODY
IMMUNOGLOBULIN G ANTIBODY
PROTEIN ESPB
RECOMBINANT PROTEIN
UNCLASSIFIED DRUG
ANIMAL EXPERIMENT
ANIMAL MODEL
BACTERIAL SHEDDING
CATTLE
CONTROLLED STUDY
ESCHERICHIA COLI INFECTION
ESCHERICHIA COLI O157
IMMUNOGLOBULIN BLOOD LEVEL
MALE
NONHUMAN
ADHESINS, BACTERIAL
ANIMALS
ANTIBODIES, BACTERIAL
BACTERIAL OUTER MEMBRANE PROTEINS
CATTLE DISEASES
ESCHERICHIA COLI INFECTIONS
ESCHERICHIA COLI PROTEINS
ESCHERICHIA COLI VACCINES
FECES
INJECTIONS, INTRAMUSCULAR
RECOMBINANT PROTEINS
TREATMENT OUTCOME
VACCINATION
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2011Vilte.pdf
LINK AL EDITOR
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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245 1 0 |a Reduced faecal shedding of Escherichia coli O157  |b H7 in cattle following systemic vaccination with Y - intimin C 280 and EspB proteins 
520 |a Enterohaemorrhagic Escherichia coli [EHEC] O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome [HUS] worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of gamma-intimin [gamma-intimin C 280] and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle. 
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653 0 |a FECES 
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773 |t Vaccine  |g vol.29, no.23 (2011), p.3962-3968 
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900 |a ^aVilte^bD.A.^tInstituto de Patobiología, Instituto Nacional de Tecnología Agropecuaria [INTA], Los Reseros y Las Cabañas, 1712 Castelar, Argentina 
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900 |a Enterohaemorrhagic Escherichia coli [EHEC] O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome [HUS] worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of gamma-intimin [gamma-intimin C 280] and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle. 
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