Characterization of small - spored alternaria from Argentinean crops through a polyphasic approach
Small-spored Alternaria have been isolated from a wide variety of food crops, causing both economic losses and human health risk due to the metabolites produced. Their taxonomy has been discussed widely, but no scientific consensus has been established in this field to date. Argentina is a major exp...
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| Otros Autores: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Materias: | |
| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2017dacruzcabral.pdf LINK AL EDITOR |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
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| 003 | AR-BaUFA | ||
| 005 | 20180629140718.0 | ||
| 008 | 180522t2017 ne |||||o|||| 00| | eng d | ||
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| 999 | |d 45517 | ||
| 022 | |a 0168-1605 | ||
| 024 | |a 10.1016/j.ijfoodmicro.2017.06.026 | ||
| 040 | |a AR-BaUFA | ||
| 245 | 1 | |a Characterization of small - spored alternaria from Argentinean crops through a polyphasic approach | |
| 520 | |a Small-spored Alternaria have been isolated from a wide variety of food crops, causing both economic losses and human health risk due to the metabolites produced. Their taxonomy has been discussed widely, but no scientific consensus has been established in this field to date. Argentina is a major exporter of agricultural products, so it is essential to thoroughly understand the physiological behaviour of this pathogen in a food safety context. Thus, the objective of this work was to characterize small-spored Alternaria spp. obtained from tomato fruits, pepper fruits, wheat grains and blueberries from Argentina by a polyphasic approach involving metabolomic and phylogenetic analyses based on molecular and morphological characters. Morphological analysis divided the population studied into three groups; A. arborescens sp.-grp., A. tenuissima sp.-grp., and A. alternata sp.-grp. However, when these characters were simultaneously analysed with molecular data, no clearly separated groups were obtained. Haplotype network and phylogenetic analysis (both Bayesian and maximum parsimony) of a conserved region yielded the same result, suggesting that all isolates belong to the same species. Furthermore, no correlation could be established between morphological species groups and a metabolite or group of metabolites synthesized. Thus, the whole set of analyses carried out in the present work supports the hypothesis that these small-spored Alternaria isolates from food belong to the same species. Identification at species level through classical morphology or modern molecular techniques does not seem to be a useful tool to predict toxicological risk in food matrices. The detection of any small spored Alternaria from Section Alternaria (D.P. Lawr., Gannibal, Peever & B.M. Pryor 2013) in food implies a potential toxicological risk. | ||
| 653 | |a ALTERNARIA | ||
| 653 | |a ENDOPOLYGALACTURONASE GENE | ||
| 653 | |a POLYPHASIC APPROACH | ||
| 653 | |a METABOLITES PROFILE | ||
| 653 | |a FOOD | ||
| 700 | 1 | |a Cruz Cabral, Lucía da |u Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Laboratorio de Microbiología de Alimentos, Buenos Aires, Argentina. E-mail: ldacruzcabral@qo.fcen.uba.ar |9 67182 | |
| 700 | 1 | |a Rodriguero, Marcela |u Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA - CONICET/UBA), Grupos de Investigación en Filogeografía y Filogenias Moleculares, Buenos Aires, Argentina |9 67183 | |
| 700 | 1 | |a Stenglein, Sebastián |u Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CICBA-INBIOTEC, CONICET, Área de Microbiología, Facultad de Agronomía, UNCPBA, Azul, Buenos Aires, Argentina |9 67184 | |
| 700 | 1 | |a Fog Nielsen, Kristian |u Technical University of Denmark, Department of Systems Biology, Kgs. Lyngby, Denmark |9 67185 | |
| 700 | 1 | |a Patriarca, Andrea |u Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Laboratorio de Microbiología de Alimentos, Buenos Aires, Argentina |9 67186 | |
| 773 | |t International Journal of Food Microbiology |g Vol.257 (2017), p. 206-215, grafs., tbls. | ||
| 856 | |f 2017dacruzcabral |i en reservorio |u http://ri.agro.uba.ar/files/intranet/articulo/2017dacruzcabral.pdf |q application/pdf |x ARTI201806 | ||
| 856 | |z LINK AL EDITOR |u http://www.elsevier.com | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||