Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells
Bovine tuberculosis is an important animal and zoonotic disease caused by Mycobacterium bovis. The innate immune response is the first line of defense against pathogens and is also crucial for the development of an efficient adaptive immune response. In this study we used an in vitro co-culture mode...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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| Acceso en línea: | http://ri.agro.uba.ar/files/download/articulo/2021blanco.pdf LINK AL EDITOR |
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| 022 | |a 1664-3224 | ||
| 024 | |a 10.3389/fimmu.2021.674643 | ||
| 040 | |a AR-BaUFA |c AR-BaUFA | ||
| 245 | 1 | |a Identifying bacterial and host factors Involved in the interaction of Mycobacterium bovis with the bovine innate immune cells | |
| 520 | |a Bovine tuberculosis is an important animal and zoonotic disease caused by Mycobacterium bovis. The innate immune response is the first line of defense against pathogens and is also crucial for the development of an efficient adaptive immune response. In this study we used an in vitro co-culture model of antigen presenting cells (APC) and autologous lymphocytes derived from peripheral blood mononuclear cells to identify the cell populations and immune mediators that participate in the development of an efficient innate response capable of controlling the intracellular replication of M. bovis. After M. bovis infection, bovine immune cell cultures displayed upregulated levels of iNOS, IL-22 and IFN-g and the induction of the innate immune response was dependent on the presence of differentiated APC. Among the analyzed M. bovis isolates, only a live virulent M. bovis isolate induced an efficient innate immune response, which was increased upon stimulation of cell co-cultures with the M. bovis culture supernatant. Moreover, we demonstrated that an allelic variation of the early secreted protein ESAT-6 (ESAT6 T63A) expressed in the virulent strain is involved in this increased innate immune response. These results highlight the relevance of the compounds secreted by live M. bovis as well as the variability among the assessed M. bovis strains to induce an efficient innate immune response. | ||
| 650 | |2 Agrovoc |9 26 | ||
| 653 | |a INNATE IMMUNE | ||
| 653 | |a MYCOBACTERIUM BOVIS | ||
| 653 | |a NK CELLS | ||
| 653 | |a ESAT-6 | ||
| 653 | |a BOVINE TUBERCULOSIS | ||
| 700 | 1 | |a Blanco, Federico Carlos |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina. |u CONICET. Buenos Aires, Argentina. |9 70699 | |
| 700 | 1 | |a Gravisaco, María José |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina. |9 69464 | |
| 700 | 1 | |a Bigi, María Mercedes |u Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina. |9 67558 | |
| 700 | 1 | |a García, Elizabeth Andrea |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina. |9 74092 | |
| 700 | 1 | |a Marquez, Cecilia |u High Technology Analytical Centre. Laboratory. Buenos Aires, Argentina. |9 74093 | |
| 700 | 1 | |a McNeil, Mike |u Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States. |9 74094 | |
| 700 | 1 | |a Jackson, Mary |u Colorado State University. Department of Microbiology. Immunology and Pathology. Fort Collins, CO, United States. |9 74095 | |
| 700 | 1 | |a Bigi, Fabiana |u Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina. |u CONICET. Buenos Aires, Argentina. |9 48059 | |
| 773 | 0 | |t Frontiers in Immunology |g Vol.12 (2021), e 674643, 16 p., grafs., tbls. | |
| 856 | |f 2021blanco |i en internet |q application/pdf |u http://ri.agro.uba.ar/files/download/articulo/2021blanco.pdf |x ARTI202210 | ||
| 856 | |u http://www.frontiersin.org/ |z LINK AL EDITOR | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||