Valorization of an Andean crop (yacon) through the production of a yeast cell - bound phytase
The suitability of the yeast Schwanniomyces (f. Debaryomyces) occidentalis to produce phytase activity on a Smallanthus sonchifolius (yacon) extract-based medium (YSM) was studied. Optimal conditions for phytase production were evaluated in YSM at 28 ◦C and pH 5.5, with an inoculum of 107 cells/m...
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| Acceso en línea: | http://ri.agro.uba.ar/files/intranet/articulo/2021condemolina.pdf LINK AL EDITOR |
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| 245 | 1 | |a Valorization of an Andean crop (yacon) through the production of a yeast cell - bound phytase | |
| 520 | |a The suitability of the yeast Schwanniomyces (f. Debaryomyces) occidentalis to produce phytase activity on a Smallanthus sonchifolius (yacon) extract-based medium (YSM) was studied. Optimal conditions for phytase production were evaluated in YSM at 28 ◦C and pH 5.5, with an inoculum of 107 cells/mL, in shaken flasks, using a Box-Benkhen optimization design, and analyzed by response surface methodology. Phytase location was determined by subcellular fractioning and protoplast formation. Maximum phytase titres were obtained with 0.11% w/v yacon reducing sugar, 0.65% w/v (NH4)2SO4 and 16 h of fermentation. The maximum phytase activity attained under these conditions was over 6-fold higher than in non-optimized medium. Scaling-up in a STR-type bioreactor further increased phytase productivity by 1.5-fold. Phytase produced was associated to the cell wall fraction, and presented optimal temperature and pH range values of 75–80 ◦C and 4.0–5.0, respectively, retaining 80% of its activity at 80 ◦C for 40 min. Progressively increasing phosphorus concentration in the medium inhibited enzyme production, up to 60% with 0.1% w/v KH2PO4. Yeast biomass, phytase production and production kinetics in YSM were similar to those produced in a cane molasses-based medium. Yacon resulted in a promising alternative feed stock to produce a yeast-bound phytase potentially applicable in the feed industry as a nutritional additive. | ||
| 650 | |2 Agrovoc |9 26 | ||
| 653 | |a FEED INDUSTRY | ||
| 653 | |a NUTRITIONAL ADDITIVE | ||
| 653 | |a YEAST | ||
| 653 | |a PHYTASE | ||
| 653 | |a SCHWANNIOMYCES OCCIDENTALIS | ||
| 653 | |a SMALLANTHUS SONCHIFOLIUS (YACON) | ||
| 700 | 1 | |a Conde Molina, Débora |u Universidad Tecnológica Nacional. Facultad Regional Delta. Departamento de Ingeniería Química. Laboratorio de Biotecnología. Campana, Buenos Aires, Argentina. |9 74082 | |
| 700 | 1 | |a Novelli Poisson, Guido Fernando |u Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Ingeniería Química. Laboratorio de Microbiología Industrial. Ciudad Universitaria, Buenos Aires, Argentina. |u Universidad Nacional de San Martín (UNSAM). Instituto de Investigaciones Biotecnológicas (IIBIO). San Martín, Buenos Aires, Argentina. |u CONICET - Universidad Nacional de San Martín (UNSAM). Instituto de Investigaciones Biotecnológicas (IIBIO). San Martín, Buenos Aires, Argentina. |9 74083 | |
| 700 | 1 | |a Kronberg, María Florencia |u Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. |u CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. |9 66853 | |
| 700 | 1 | |a Galvagno, Miguel Angel |u Universidad Nacional de San Martín (UNSAM). Instituto de Investigaciones Biotecnológicas (IIBIO). San Martín, Buenos Aires, Argentina. |u Universidad Nacional de San Martín (UNSAM). Instituto de Investigaciones Biotecnológicas (IIBIO). San Martín, Buenos Aires, Argentina. |u Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica (INMIBO). Ciudad Universitaria, Buenos Aires, Argentina. |u CONICET - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica (INMIBO). Ciudad Universitaria, Buenos Aires, Argentina. |9 69520 | |
| 773 | 0 | |t Biocatalysis and Agricultural Biotecnology |g Vol.36 (2021), e102116, 8 p., grafs., tbls. | |
| 856 | |f 2021condemolina |i en reservorio |q application/pdf |u http://ri.agro.uba.ar/files/intranet/articulo/2021condemolina.pdf |x ARTI202210 | ||
| 856 | |u https://www.elsevier.com/ |z LINK AL EDITOR | ||
| 942 | |c ARTICULO | ||
| 942 | |c ENLINEA | ||
| 976 | |a AAG | ||