Cholestenes HEK293 Cells Humans Ligands in vitro study ligand ligand binding molecular dynamics unclassified drug 27 nor 5 cholestenoic acid 27 nor delta4 dafachronic acid Animals Article Binding Sites Caenorhabditis elegans Caenorhabditis elegans Proteins Cholestenoic acid DAF-12 receptor Caenorhabditis elegans daf-9 Mutants Dafachronic acid Molecular dynamics Dose-Response Relationship, Drug Gene Expression Regulation HEK293 cell line Hep G2 Cells HepG2 cell line Inverse agonism Liver X receptors Models, Molecular Molecular Conformation Molecular Dynamics Simulation Molecular dynamics Orphan Nuclear Receptors