article Humans Circular Dichroism Homeostasis Hydrodynamics Iron Iron-Binding Proteins Magnetic Resonance Spectroscopy Microscopy, Fluorescence Models, Molecular Molecular Conformation Molecular Dynamics Simulation Point Mutation Protein Denaturation Protein Folding Protein Structure, Tertiary Recombinant Proteins Solvents Temperature Time Factors alpha helix carboxy terminal sequence circular dichroism fluorescence analysis frataxin hydrodynamics nuclear magnetic resonance spectroscopy protein folding protein function protein secondary structure