Blotting, Western 1 Calcium 1 Fluorescence Resonance Energy Transfer 1 HEK293 Cells 1 HEK293 cell line 1 Humans 1 Immunoprecipitation 1 P2XR receptor, human 1 Patch-Clamp Techniques 1 Polymorphism, Single Nucleotide 1 RNA, Small Interfering 1 Real-Time Polymerase Chain Reaction 1 Receptors, Purinergic P2X7 1 Signal Transduction 1 Western blotting 1 calcium 1 fluorescence resonance energy transfer 1 genetics 1 human 1 immunoprecipitation 1 metabolism 1 patch clamp technique 1 physiology 1 purinergic P2X7 receptor 1 real time polymerase chain reaction 1 signal transduction 1 single nucleotide polymorphism 1 small interfering RNA 1