Stability of enzymes and proteins in dried glassy systems: Effect of simulated sunlight conditions

The purpose of the present work was to study the effects of simulated sunlight conditions on enzyme inactivation and structural damage in dehydrated glassy systems. Freeze-dried samples containing different enzymes (lactase, invertase, lysozyme and amyloglucosidase) were exposed to light using a med...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Espinosa, L., Schebor, C., Nudelman, N.S., Chirife, J.
Formato:	JOUR
Materias:	Dehydration Electrophoresis Enzyme kinetics Enzymes Freezing Glass Solar energy Dried glassy systems Enzyme inactivation Structural damage Biotechnology enzyme glass protein article chemistry differential scanning calorimetry enzyme stability polyacrylamide gel electrophoresis protein conformation sunlight Calorimetry, Differential Scanning Electrophoresis, Polyacrylamide Gel Enzyme Stability Protein Conformation Proteins Sunlight
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_87567938_v20_n4_p1220_Espinosa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

Ejemplares similares

Stability of enzymes and proteins in dried glassy systems: Effect of simulated sunlight conditions
por: Schebor, Carolina C.
Publicado: (2004)

Calorimetric studies on dry pectinlyase preparations: Impact of glass transition on inactivation kinetics
por: Taragano, Viviana M., et al.
Publicado: (2001)

Calorimetric studies on dry pectinlyase preparations: Impact of glass transition on inactivation kinetics
por: Taragano, V.M., et al.

Thermal resistance of β-galactosidase in dehydrated dairy model systems as affected by physical and chemical changes
por: Burin, L., et al.

Polymeric structure of the cyclic AMP-dependent protein kinase from the dimorphic fungus Mucor rouxii and purification of its catalytic subunit
por: Pastori, Ricardo Luis, et al.
Publicado: (1985)

Polymeric structure of the cyclic AMP-dependent protein kinase from the dimorphic fungus Mucor rouxii and purification of its catalytic subunit
por: Pastori, R., et al.

DSC confirmation that vitrification is not necessary for stabilization of the restriction enzyme EcoRI dried with saccharides
por: Buera, M.P., et al.

Thermal resistance of β-galactosidase in dehydrated dairy model systems as affected by physical and chemical changes
por: Burín, Leila, et al.
Publicado: (2002)

DSC confirmation that vitrification is not necessary for stabilization of the restriction enzyme EcoRI dried with saccharides
por: Buera, María del Pilar, et al.
Publicado: (1999)

Endoglucanase production in 4 species of the genus Saccobolus
por: Ramos, Araceli Marcela, et al.
Publicado: (1996)

Endoglucanase production in 4 species of the genus Saccobolus
por: Ramos, A.M., et al.

Rat kidney porphobilinogen deaminase kinetics: Detection of enzyme-substrate complexes
por: Noriega, Guillermo Osvaldo, et al.
Publicado: (2002)

Rat kidney porphobilinogen deaminase kinetics: Detection of enzyme-substrate complexes
por: Noriega, G., et al.

Concerted action of cosolvents, chaotropic anions and thioredoxin on chloroplast fructose-1,6-bisphosphatase. Reactivity to iodoacetamide.
por: Stein, Mariana, et al.
Publicado: (1989)

Concerted action of cosolvents, chaotropic anions and thioredoxin on chloroplast fructose-1,6-bisphosphatase. Reactivity to iodoacetamide.
por: Stein, M., et al.

Phase transitions and functionality of enzymes and yeasts in dehydrated matrices
por: Mazzobre, María Florencia, et al.
Publicado: (2003)

Phase transitions and functionality of enzymes and yeasts in dehydrated matrices
por: Mazzobre, M.F., et al.

Influence of complexing carboxymethylcellulose on the thermostability and gelation of α-lactalbumin and β-lactoglobulin
por: Pilosof, Ana María Renata
Publicado: (2007)

Influence of complexing carboxymethylcellulose on the thermostability and gelation of α-lactalbumin and β-lactoglobulin
por: Capitani, C., et al.

Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites
por: Matiacevich, Silvia Beatriz, et al.
Publicado: (2008)

Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites
por: Desimone, M.F., et al.

Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems
Publicado: (1999)

Combined effects of trehalose and cations on the thermal resistance of β-galactosidase in freeze-dried systems
por: Mazzobre, M.F., et al.

An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
por: Tandecarz, Juana Sara
Publicado: (1998)

An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
por: García-Patrone, M., et al.

Isolation, purification and some properties of an extracellular phbv depolymerase from Streptomyces SP. SSM 5670
por: Yashchuk, O.

Isolation, purification and some properties of an extracellular phbv depolymerase from Streptomyces SP. SSM 5670
por: Yashchuk, O.

β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions
por: Santagapita, Patricio Román, et al.
Publicado: (2011)

β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions
por: Santagapita, P.R., et al.

Effects of organic solvents on immobilized lipase in pectin microspheres
por: Costas, L., et al.
Publicado: (2008)

Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?
Publicado: (1982)

Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?
por: Olavarría, J.

Pulsed electric fields effects on the molecular structure and gelation of β-lactoglobulin concentrate and egg white
por: Pérez, Oscar E., et al.
Publicado: (2004)

Pulsed electric fields effects on the molecular structure and gelation of β-lactoglobulin concentrate and egg white
por: Perez, O.E., et al.

Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
por: Santagapita, Patricio Román, et al.
Publicado: (2015)

Impact of supramolecular interactions of dextran-β-cyclodextrin polymers on invertase activity in freeze-dried systems
por: Santagapita, P.R., et al.

Recognition-driven assembly of self-limiting supramolecular protein nanoparticles displaying enzymatic activity
por: Pallarola, Diego Andrés, et al.
Publicado: (2015)

Recognition-driven assembly of self-limiting supramolecular protein nanoparticles displaying enzymatic activity
por: Piccinini, E., et al.

Circumscription of the south American moonwort botrychium (ophioglossaceae)
por: Meza Torres, Esteban I., et al.
Publicado: (2022)

Water-dependent thermal transitions in quinoa embryos
por: Matiacevich, Silvia Beatriz, et al.
Publicado: (2006)