Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution

Herein, we report on the implementation of photofunctional surfaces for the investigation of cellular responses by means of quantitative fluorescence microscopy. The developed substrates are able to produce reactive oxygen species under the fluorescence microscope upon irradiation with visible light...

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Detalles Bibliográficos
Autores principales: Stegemann, L., Schuermann, K.C., Strassert, C.A., Grecco, H.E.
Formato: JOUR
Materias:
apoptosis
caspase activity
cell death
necrosis
photoactive surfaces
phototherapy
quantitative functional microscopy
reactive oxygen species
Cell death
Cells
Cytology
Diseases
Fluorescence
Fluorescence microscopy
Mobile security
Morphology
Oxygen
Photosensitizers
Caspases
Phototherapy
Quantitative evaluation
Quantitative fluorescence microscopy
Reactive oxygen species
Spatio-temporal resolution
Time resolved fluorescence anisotropy
Molecular oxygen
nanoparticle
reactive oxygen metabolite
chemistry
fluorescence microscopy
HeLa cell line
human
light
metabolism
molecular imaging
photochemistry
procedures
radiation response
spatiotemporal analysis
HeLa Cells
Humans
Light
Microscopy, Fluorescence
Molecular Imaging
Nanoparticles
Photochemistry
Reactive Oxygen Species
Spatio-Temporal Analysis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19448244_v7_n10_p5944_Stegemann
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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