Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5
Fusaric acid is produced by pathogenic fungi of the genus Fusarium, and is toxic to plants and rhizobacteria. Many fluorescent pseudomonads can prevent wilt diseases caused by these fungi. This study was undertaken to evaluate the effect of fusaric acid on P. protegens Pf-5 and elucidate the mechani...
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todo:paper_19326203_v10_n1_p_Ruiz2023-10-03T16:34:34Z Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 Ruiz, J.A. Bernar, E.M. Jung, K. copper ion enantio pyochelin ferric ion ferrous ion fusaric acid manganese microbial products not classified elsewhere multidrug resistance protein pyoverdine siderophore unclassified drug zinc ion bacterial protein fusaric acid ion iron metal oligopeptide pyoverdine siderophore antibacterial activity antibiotic resistance Article bacterial gene bacterial growth bacterial secretion system bacterial strain bacterial survival bacterium culture bacterium mutant binding affinity binding kinetics biofilm biosynthesis cell motility concentration response controlled study drug binding fus gene gene expression growth inhibition minimum inhibitory concentration nonhuman protein expression Pseudomonas Pseudomonas protegens spectrophotometry wild type chemistry drug effects growth, development and aging metabolism physiology Pseudomonas spectrofluorometry Bacteria (microorganisms) Fungi Fusarium Pseudomonas Pseudomonas fluorescens group Rhizobiales Bacterial Proteins Biofilms Fusaric Acid Ions Iron Metals Oligopeptides Pseudomonas Siderophores Spectrometry, Fluorescence Fusaric acid is produced by pathogenic fungi of the genus Fusarium, and is toxic to plants and rhizobacteria. Many fluorescent pseudomonads can prevent wilt diseases caused by these fungi. This study was undertaken to evaluate the effect of fusaric acid on P. protegens Pf-5 and elucidate the mechanisms that enable the bacterium to survive in the presence of the mycotoxin. The results confirm that fusaric acid negatively affects growth and motility of P. protegens. Moreover, a notable increase in secretion of the siderophore pyoverdine was observed when P. protegens was grown in the presence of fusaric acid. Concomitantly, levels of enzymes involved in the biosynthesis of pyoverdine and enantio-pyochelin, the second siderophore encoded by P. protegens, increased markedly. Moreover, while similar levels of resistance to fusaric acid were observed for P. protegens mutants unable to synthesize either pyoverdine or enanto-pyochelin and the wild type strain, a double mutant unable to synthesize both kinds of siderophores showed a dramatically reduced resistance to this compound. This reduced resistance was not observed when this mutant was grown under conditions of iron excess. Spectrophotometric titrations revealed that fusaric acid binds not only Fe2+ and Fe3+, but also Zn2+, Mn2+ and Cu2+, with high affinity. Our results demonstrate that iron sequestration accounts at least in part for the deleterious effect of the mycotoxin on P. protegens. © 2015 Ruiz et al. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v10_n1_p_Ruiz |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
copper ion enantio pyochelin ferric ion ferrous ion fusaric acid manganese microbial products not classified elsewhere multidrug resistance protein pyoverdine siderophore unclassified drug zinc ion bacterial protein fusaric acid ion iron metal oligopeptide pyoverdine siderophore antibacterial activity antibiotic resistance Article bacterial gene bacterial growth bacterial secretion system bacterial strain bacterial survival bacterium culture bacterium mutant binding affinity binding kinetics biofilm biosynthesis cell motility concentration response controlled study drug binding fus gene gene expression growth inhibition minimum inhibitory concentration nonhuman protein expression Pseudomonas Pseudomonas protegens spectrophotometry wild type chemistry drug effects growth, development and aging metabolism physiology Pseudomonas spectrofluorometry Bacteria (microorganisms) Fungi Fusarium Pseudomonas Pseudomonas fluorescens group Rhizobiales Bacterial Proteins Biofilms Fusaric Acid Ions Iron Metals Oligopeptides Pseudomonas Siderophores Spectrometry, Fluorescence |
| spellingShingle |
copper ion enantio pyochelin ferric ion ferrous ion fusaric acid manganese microbial products not classified elsewhere multidrug resistance protein pyoverdine siderophore unclassified drug zinc ion bacterial protein fusaric acid ion iron metal oligopeptide pyoverdine siderophore antibacterial activity antibiotic resistance Article bacterial gene bacterial growth bacterial secretion system bacterial strain bacterial survival bacterium culture bacterium mutant binding affinity binding kinetics biofilm biosynthesis cell motility concentration response controlled study drug binding fus gene gene expression growth inhibition minimum inhibitory concentration nonhuman protein expression Pseudomonas Pseudomonas protegens spectrophotometry wild type chemistry drug effects growth, development and aging metabolism physiology Pseudomonas spectrofluorometry Bacteria (microorganisms) Fungi Fusarium Pseudomonas Pseudomonas fluorescens group Rhizobiales Bacterial Proteins Biofilms Fusaric Acid Ions Iron Metals Oligopeptides Pseudomonas Siderophores Spectrometry, Fluorescence Ruiz, J.A. Bernar, E.M. Jung, K. Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| topic_facet |
copper ion enantio pyochelin ferric ion ferrous ion fusaric acid manganese microbial products not classified elsewhere multidrug resistance protein pyoverdine siderophore unclassified drug zinc ion bacterial protein fusaric acid ion iron metal oligopeptide pyoverdine siderophore antibacterial activity antibiotic resistance Article bacterial gene bacterial growth bacterial secretion system bacterial strain bacterial survival bacterium culture bacterium mutant binding affinity binding kinetics biofilm biosynthesis cell motility concentration response controlled study drug binding fus gene gene expression growth inhibition minimum inhibitory concentration nonhuman protein expression Pseudomonas Pseudomonas protegens spectrophotometry wild type chemistry drug effects growth, development and aging metabolism physiology Pseudomonas spectrofluorometry Bacteria (microorganisms) Fungi Fusarium Pseudomonas Pseudomonas fluorescens group Rhizobiales Bacterial Proteins Biofilms Fusaric Acid Ions Iron Metals Oligopeptides Pseudomonas Siderophores Spectrometry, Fluorescence |
| description |
Fusaric acid is produced by pathogenic fungi of the genus Fusarium, and is toxic to plants and rhizobacteria. Many fluorescent pseudomonads can prevent wilt diseases caused by these fungi. This study was undertaken to evaluate the effect of fusaric acid on P. protegens Pf-5 and elucidate the mechanisms that enable the bacterium to survive in the presence of the mycotoxin. The results confirm that fusaric acid negatively affects growth and motility of P. protegens. Moreover, a notable increase in secretion of the siderophore pyoverdine was observed when P. protegens was grown in the presence of fusaric acid. Concomitantly, levels of enzymes involved in the biosynthesis of pyoverdine and enantio-pyochelin, the second siderophore encoded by P. protegens, increased markedly. Moreover, while similar levels of resistance to fusaric acid were observed for P. protegens mutants unable to synthesize either pyoverdine or enanto-pyochelin and the wild type strain, a double mutant unable to synthesize both kinds of siderophores showed a dramatically reduced resistance to this compound. This reduced resistance was not observed when this mutant was grown under conditions of iron excess. Spectrophotometric titrations revealed that fusaric acid binds not only Fe2+ and Fe3+, but also Zn2+, Mn2+ and Cu2+, with high affinity. Our results demonstrate that iron sequestration accounts at least in part for the deleterious effect of the mycotoxin on P. protegens. © 2015 Ruiz et al. |
| format |
JOUR |
| author |
Ruiz, J.A. Bernar, E.M. Jung, K. |
| author_facet |
Ruiz, J.A. Bernar, E.M. Jung, K. |
| author_sort |
Ruiz, J.A. |
| title |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_short |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_full |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_fullStr |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_full_unstemmed |
Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5 |
| title_sort |
production of siderophores increases resistance to fusaric acid in pseudomonas protegens pf-5 |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v10_n1_p_Ruiz |
| work_keys_str_mv |
AT ruizja productionofsiderophoresincreasesresistancetofusaricacidinpseudomonasprotegenspf5 AT bernarem productionofsiderophoresincreasesresistancetofusaricacidinpseudomonasprotegenspf5 AT jungk productionofsiderophoresincreasesresistancetofusaricacidinpseudomonasprotegenspf5 |
| _version_ |
1807315893912338432 |