Quantifying transcription factor-DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy

Probing transcription factor (TF)-DNA interactions remains challenging in complex in vivo systems such as mammalian embryos, especially when TF copy numbers and fluorescence background are high. To address this difficulty, fluorescence correlation spectroscopy (FCS) can be combined with the use of p...

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Detalles Bibliográficos
Autores principales: Zhao, Z.W., White, M.D., Alvarez, Y.D., Zenker, J., Bissiere, S., Plachta, N.
Formato: JOUR
Materias:
DNA
fluorescent dye
transcription factor
protein binding
animal cell
animal experiment
animal tissue
Article
clinical protocol
DNA binding
embryo
embryo culture
embryo development
female
fluorescence correlation spectroscopy
in vivo study
information processing
mouse
nonhuman
photoactivation
priority journal
process optimization
protein DNA binding
protein protein interaction
quantitative analysis
single cell analysis
animal
mammalian embryo
metabolism
procedures
spectrofluorometry
time factor
Animals
Embryo, Mammalian
Mice
Protein Binding
Single-Cell Analysis
Spectrometry, Fluorescence
Time Factors
Transcription Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_17542189_v12_n7_p1458_Zhao
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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