Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells

Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferat...

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Detalles Bibliográficos
Autores principales: Albani, C.M., Denegri, G.M., Elissondo, M.C.
Formato: JOUR
Materias:
anthelmintic agent
essential oil
thymol
anthelmintic activity
Article
cell count
cell proliferation
cell survival
cell viability
controlled study
drug effect
drug mechanism
Echinococcus granulosus
in vitro study
Mentha piperita
Mentha pulegium
nonhuman
rosemary
scanning electron microscopy
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1687708X_v2014_n_p_Albani
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_1687708X_v2014_n_p_Albani
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spelling todo:paper_1687708X_v2014_n_p_Albani2023-10-03T16:29:53Z Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells Albani, C.M. Denegri, G.M. Elissondo, M.C. anthelmintic agent essential oil thymol anthelmintic activity Article cell count cell proliferation cell survival cell viability controlled study drug effect drug mechanism Echinococcus granulosus in vitro study Mentha piperita Mentha pulegium nonhuman rosemary scanning electron microscopy Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus larval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst's germinal layer and exposed to 1, 5, and 10 g/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 106 (100%) after 7 days of incubation. At day 7, thymol 5 g/ml caused a reduction in cell viability of 63% and the essential oils of M. piperita 10 g/ml, M. pulegium 10 g/ml, and R. officinalis 10 g/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils on E. granulosus cells we suggested that it would be an effective means for suppression of larval growth. © 2014 Clara María Albani et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1687708X_v2014_n_p_Albani
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic anthelmintic agent
essential oil
thymol
anthelmintic activity
Article
cell count
cell proliferation
cell survival
cell viability
controlled study
drug effect
drug mechanism
Echinococcus granulosus
in vitro study
Mentha piperita
Mentha pulegium
nonhuman
rosemary
scanning electron microscopy
spellingShingle anthelmintic agent
essential oil
thymol
anthelmintic activity
Article
cell count
cell proliferation
cell survival
cell viability
controlled study
drug effect
drug mechanism
Echinococcus granulosus
in vitro study
Mentha piperita
Mentha pulegium
nonhuman
rosemary
scanning electron microscopy
Albani, C.M.
Denegri, G.M.
Elissondo, M.C.
Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
topic_facet anthelmintic agent
essential oil
thymol
anthelmintic activity
Article
cell count
cell proliferation
cell survival
cell viability
controlled study
drug effect
drug mechanism
Echinococcus granulosus
in vitro study
Mentha piperita
Mentha pulegium
nonhuman
rosemary
scanning electron microscopy
description Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus larval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst's germinal layer and exposed to 1, 5, and 10 g/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 106 (100%) after 7 days of incubation. At day 7, thymol 5 g/ml caused a reduction in cell viability of 63% and the essential oils of M. piperita 10 g/ml, M. pulegium 10 g/ml, and R. officinalis 10 g/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils on E. granulosus cells we suggested that it would be an effective means for suppression of larval growth. © 2014 Clara María Albani et al.
format JOUR
author Albani, C.M.
Denegri, G.M.
Elissondo, M.C.
author_facet Albani, C.M.
Denegri, G.M.
Elissondo, M.C.
author_sort Albani, C.M.
title Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
title_short Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
title_full Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
title_fullStr Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
title_full_unstemmed Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
title_sort effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
url http://hdl.handle.net/20.500.12110/paper_1687708X_v2014_n_p_Albani
work_keys_str_mv AT albanicm effectofdifferentterpenecontainingessentialoilsontheproliferationofechinococcusgranulosuslarvalcells
AT denegrigm effectofdifferentterpenecontainingessentialoilsontheproliferationofechinococcusgranulosuslarvalcells
AT elissondomc effectofdifferentterpenecontainingessentialoilsontheproliferationofechinococcusgranulosuslarvalcells
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