Pollen aquaporins: What are they there for?

In order to provide more insight into the function of aquaporins during pollination, we characterized NIP4;1 and NIP4;2, 2 pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 displayed high amino acid identity. RT-PCR and GUS promoter analysis showed that they have different expres...

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Detalles Bibliográficos
Autores principales: Pérez Di Giorgio, J.A., Barberini, M.L., Amodeo, G., Muschietti, J.P.
Formato: JOUR
Materias:
Aquaporin
pollen
pollen hydration
pollination
aquaporin
Arabidopsis protein
Arabidopsis
genetics
metabolism
physiology
pollen tube
promoter region
Aquaporins
Arabidopsis Proteins
Pollen Tube
Pollination
Promoter Regions, Genetic
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15592324_v11_n9_pe1217375_PerezDiGiorgio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:In order to provide more insight into the function of aquaporins during pollination, we characterized NIP4;1 and NIP4;2, 2 pollen-specific aquaporins of Arabidopsis thaliana. NIP4;1 and NIP4;2 displayed high amino acid identity. RT-PCR and GUS promoter analysis showed that they have different expression patterns. NIP4;1 is expressed at low levels in mature pollen, while NIP4;2 is highly expressed only during pollen tube growth. Single T-DNA nip4;1 and nip4;2 mutants and double amiRNA nip4;1 nip4;2 knockdowns showed reduced male fertility due to deficient pollen germination and pollen tube length. Functional assays in oocytes showed that NIP4;1 and NIP4;2 transport water and nonionic solutes. Here, the participation of the different pollen aquaporins in pollen hydration and pollen tube growth is discussed.

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