Nitric oxide sets off an antioxidant response in adrenal cells: Involvement of sGC and Nrf2 in HO-1 induction

Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulato...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Astort, F., Mercau, M., Giordanino, E., Degese, M.S., Caldareri, L., Coso, O., Cymeryng, C.B.
Formato: JOUR
Materias:
cGMP
Heme oxygenase 1
Nitric oxide
Nuclear factor-erythroid 2-related factor (Nrf2)
Soluble guanylate cyclase
cyclic GMP
dactinomycin
diethylenetriamine
guanylate cyclase
guanylate cyclase inhibitor
heme oxygenase 1
messenger RNA
nitric oxide
transcription factor Nrf2
antioxidant
Nfe2l2 protein, mouse
adrenal cortex cell
animal cell
article
cellular stress response
controlled study
enzyme induction
intracellular signaling
mouse
nonhuman
oxidative stress
priority journal
protein localization
regulatory mechanism
RNA degradation
transcription initiation
transient transfection
adrenal gland
animal
cell culture
cytology
enzymology
metabolism
solubility
Adrenal Glands
Animals
Antioxidants
Cells, Cultured
Guanylate Cyclase
Heme Oxygenase-1
Mice
NF-E2-Related Factor 2
Nitric Oxide
Solubility
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10898603_v37_n1_p1_Astort
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Induction of microsomal heme oxygenase 1 (HO-1) activity is considered a cytoprotective mechanism in different cell types. In adrenal cells, HO-1 induction by ACTH exerts a modulatory effect on steroid production as well. As nitric oxide (NO) has been also regarded as an autocrine/paracrine modulator of adrenal steroidogenesis we sought to study the effects of NO on the induction of HO-1 and the mechanism involved. We hereby analyzed the time and dose-dependent effect of a NO-donor (DETA/NO) on HO-1 induction in a murine adrenocortical cell line. We showed that this effect is mainly exerted at a transcriptional level as it is inhibited by actinomycin D and HO-1 mRNA degradation rates were not affected by DETA/NO treatment. HO-1 induction by NO does not appear to involve the generation of oxidative stress as it was not affected by antioxidant treatment. We also demonstrated that NO-treatment results in the nuclear translocation of the nuclear factor-erythroid 2-related factor (Nrf2), an effect that is attenuated by transfecting the cells with a dominant negative isoform of Nrf2. We finally show that the effects of the NO-donor are reproduced by a permeable analog of cGMP and that a soluble guanylate cyclase specific inhibitor blocked both the induction of HO-1 by NO and the nuclear translocation of Nrf2. © 2013 Elsevier Inc. All rights reserved.

Ejemplares similares