Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria

Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and...

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Detalles Bibliográficos
Autores principales: Piuri, M., Hatfull, G.F.
Formato: SER
Materias:
Antibiotics
DST
Fluoromycobacteriophage
Mycobacteria
Reporter phage
Resistance
carbenicillin
cycloheximide
ethambutol
ethionamide
isoniazid
kanamycin
rifampicin
streptomycin
tuberculostatic agent
antibiotic sensitivity
bacteriophage
flow cytometry
fluorescence microscopy
fluoromycobacteriophage
high throughput screening
mycobacteriosis
Mycobacterium
Mycobacterium smegmatis infection
nonhuman
tuberculosis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p27_Piuri
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

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