Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract

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Single-cell cytoplasm sap (1-10 pL) was extracted by using a pressure probe glass microcapillary tip from tulip leaf and bulb and analyzed by UV-MALDI-TOF MS for free underivatized carbohydrate content. Three matrices including 2,5-dihydroxybenzoic acid (DHB), 2,4,6-trihydroxyacetophenone (THAP), an...

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Detalles Bibliográficos
Autores principales: Gholipour, Y., Nonami, H., Erra-Balsells, R.
Formato: JOUR
Materias:
Carbohydrates
Carbon nanotubes
Cells
Cytology
Glucose
Military engineering
Organic compounds
Polysaccharides
Positive ions
Potential flow
Sugar (sucrose)
Sugars
Carbohydrate contents
Cell cytoplasms
Dihydroxybenzoic acids
Direct analyses
High resolutions
Leaf cells
Microcapillary tips
Positive ion modes
Pressure probes
Reproducibility
Trihydroxyacetophenone
Biomolecules
2,4,6 trihydroxyacetophenone
acetophenone derivative
carbon nanotube
cell extract
disaccharide
gentisic acid
hexose
unclassified drug
article
carbohydrate analysis
cytoplasm
matrix assisted laser desorption ionization time of flight mass spectrometry
plant cell
plant leaf
Cytoplasm
Plants
Pressure
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tulipa
Ultraviolet Rays
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10440305_v19_n12_p1841_Gholipour
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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