Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646
The fungal diversity structures of soils that are suppressive and non-suppressive to Sclerotinia sclerotiorum were characterised and screened for fungal strains antagonistic to the S. sclerotiorum pathogen. Soil suppressiveness was associated with a particular fungal diversity structure. Principal c...
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todo:paper_09583157_v25_n12_p1388_Rodriguez2023-10-03T15:52:38Z Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646 Rodríguez, M.A. Rothen, C. Lo, T.E. Cabrera, G.M. Godeas, A.M. antagonism biological control Clonostachys rosea Sclerotinia sclerotiorum antagonism antimicrobial activity bioassay fungus metabolite potential biocontrol agent principal component analysis selection soil biota soybean species diversity Bionectria ochroleuca Fusarium oxysporum Fusarium solani Glycine max Sclerotinia sclerotiorum Talaromyces flavus var. flavus The fungal diversity structures of soils that are suppressive and non-suppressive to Sclerotinia sclerotiorum were characterised and screened for fungal strains antagonistic to the S. sclerotiorum pathogen. Soil suppressiveness was associated with a particular fungal diversity structure. Principal component analysis showed that antagonism by fungal species in suppressive soils was associated with the occurrence of Fusarium oxysporum, Fusarium solani, Talaromyces flavus var. flavus and Clonostachys rosea f. rosea. In particular, C. rosea f. rosea occurred exclusively in suppressive soil samples, suggesting that this morpho-species plays an important role in suppression of S. sclerotiorum diseases. One strain of C. rosea f. rosea (BAFC1646) was selected for further experiments. Dual-culture assays confirmed the antagonistic behaviour of C. rosea f. rosea BAFC1646 against three different S. sclerotiorum strains. Antifungal activity was corroborated by diffusion assays with metabolite extracts. Greenhouse assays with soybean plants showed that the selected C. rosea f. rosea strain reduced the percentage of dead plants when co-inoculated with S. sclerotiorum. In addition, inclusion of C. rosea f. rosea alone increased shoot lengths significantly. In this work, we established the involvement of fungal species in soil suppressiveness and in further assays confirmed that C. rosea f. rosea BAFC1646 exhibits a bioprotective effect against S. sclerotiorum in soybean plants. © 2015 Taylor & Francis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09583157_v25_n12_p1388_Rodriguez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
antagonism biological control Clonostachys rosea Sclerotinia sclerotiorum antagonism antimicrobial activity bioassay fungus metabolite potential biocontrol agent principal component analysis selection soil biota soybean species diversity Bionectria ochroleuca Fusarium oxysporum Fusarium solani Glycine max Sclerotinia sclerotiorum Talaromyces flavus var. flavus |
| spellingShingle |
antagonism biological control Clonostachys rosea Sclerotinia sclerotiorum antagonism antimicrobial activity bioassay fungus metabolite potential biocontrol agent principal component analysis selection soil biota soybean species diversity Bionectria ochroleuca Fusarium oxysporum Fusarium solani Glycine max Sclerotinia sclerotiorum Talaromyces flavus var. flavus Rodríguez, M.A. Rothen, C. Lo, T.E. Cabrera, G.M. Godeas, A.M. Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646 |
| topic_facet |
antagonism biological control Clonostachys rosea Sclerotinia sclerotiorum antagonism antimicrobial activity bioassay fungus metabolite potential biocontrol agent principal component analysis selection soil biota soybean species diversity Bionectria ochroleuca Fusarium oxysporum Fusarium solani Glycine max Sclerotinia sclerotiorum Talaromyces flavus var. flavus |
| description |
The fungal diversity structures of soils that are suppressive and non-suppressive to Sclerotinia sclerotiorum were characterised and screened for fungal strains antagonistic to the S. sclerotiorum pathogen. Soil suppressiveness was associated with a particular fungal diversity structure. Principal component analysis showed that antagonism by fungal species in suppressive soils was associated with the occurrence of Fusarium oxysporum, Fusarium solani, Talaromyces flavus var. flavus and Clonostachys rosea f. rosea. In particular, C. rosea f. rosea occurred exclusively in suppressive soil samples, suggesting that this morpho-species plays an important role in suppression of S. sclerotiorum diseases. One strain of C. rosea f. rosea (BAFC1646) was selected for further experiments. Dual-culture assays confirmed the antagonistic behaviour of C. rosea f. rosea BAFC1646 against three different S. sclerotiorum strains. Antifungal activity was corroborated by diffusion assays with metabolite extracts. Greenhouse assays with soybean plants showed that the selected C. rosea f. rosea strain reduced the percentage of dead plants when co-inoculated with S. sclerotiorum. In addition, inclusion of C. rosea f. rosea alone increased shoot lengths significantly. In this work, we established the involvement of fungal species in soil suppressiveness and in further assays confirmed that C. rosea f. rosea BAFC1646 exhibits a bioprotective effect against S. sclerotiorum in soybean plants. © 2015 Taylor & Francis. |
| format |
JOUR |
| author |
Rodríguez, M.A. Rothen, C. Lo, T.E. Cabrera, G.M. Godeas, A.M. |
| author_facet |
Rodríguez, M.A. Rothen, C. Lo, T.E. Cabrera, G.M. Godeas, A.M. |
| author_sort |
Rodríguez, M.A. |
| title |
Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646 |
| title_short |
Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646 |
| title_full |
Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646 |
| title_fullStr |
Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646 |
| title_full_unstemmed |
Suppressive soil against Sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of Clonostachys rosea BAFC1646 |
| title_sort |
suppressive soil against sclerotinia sclerotiorum as a source of potential biocontrol agents: selection and evaluation of clonostachys rosea bafc1646 |
| url |
http://hdl.handle.net/20.500.12110/paper_09583157_v25_n12_p1388_Rodriguez |
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