A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde

We present a method to determine concentrations of glycine between 1 and 3 mM in the simultaneous presence of 25 -lOOm M glutamate, which is a modification of the procedure originally described by Klein and Linser, Z. Physiol. Chem. 205, 251 (1932). Glycine and glutamate react with 150 m M o-phthald...

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Autores principales:	Lombardo, M.E., Araujo, L.S., Branca, A.
Formato:	JOUR
Materias:	Glutamate Glycine o-Phthaldialdehyde
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_09395075_v45_n7-8_p911_Lombardo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_09395075_v45_n7-8_p911_Lombardo2023-10-03T15:48:49Z A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde Lombardo, M.E. Araujo, L.S. Branca, A. Glutamate Glycine o-Phthaldialdehyde We present a method to determine concentrations of glycine between 1 and 3 mM in the simultaneous presence of 25 -lOOm M glutamate, which is a modification of the procedure originally described by Klein and Linser, Z. Physiol. Chem. 205, 251 (1932). Glycine and glutamate react with 150 m M o-phthaldialdehyde, glycine is quantitatively converted into a colored o-phthaldialdehyde-glycine-glutamate complex. By recording the absorbance of the complex at its maximum peak (around 540 nm), at 600 nm and 480 nm and applying a correction formula is possible to obtain a very good linear correlation when plotting the values of corrected absorbance versus the concentration of glycine. From these plots an extinction coefficient of 7.0 * 102 cm -1 m -1 was calculated for the complex. The method is highly reproducible and sensitive and solved the problem of glutamate interference when estimating low concentrations of glycine in mixture containing 10 to 50 times concentrations of the former aminoacid. © 1990, Walter de Gruyter. All rights reserved. Fil:Lombardo, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Araujo, L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_09395075_v45_n7-8_p911_Lombardo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Glutamate Glycine o-Phthaldialdehyde
spellingShingle	Glutamate Glycine o-Phthaldialdehyde Lombardo, M.E. Araujo, L.S. Branca, A. A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde
topic_facet	Glutamate Glycine o-Phthaldialdehyde
description	We present a method to determine concentrations of glycine between 1 and 3 mM in the simultaneous presence of 25 -lOOm M glutamate, which is a modification of the procedure originally described by Klein and Linser, Z. Physiol. Chem. 205, 251 (1932). Glycine and glutamate react with 150 m M o-phthaldialdehyde, glycine is quantitatively converted into a colored o-phthaldialdehyde-glycine-glutamate complex. By recording the absorbance of the complex at its maximum peak (around 540 nm), at 600 nm and 480 nm and applying a correction formula is possible to obtain a very good linear correlation when plotting the values of corrected absorbance versus the concentration of glycine. From these plots an extinction coefficient of 7.0 * 102 cm -1 m -1 was calculated for the complex. The method is highly reproducible and sensitive and solved the problem of glutamate interference when estimating low concentrations of glycine in mixture containing 10 to 50 times concentrations of the former aminoacid. © 1990, Walter de Gruyter. All rights reserved.
format	JOUR
author	Lombardo, M.E. Araujo, L.S. Branca, A.
author_facet	Lombardo, M.E. Araujo, L.S. Branca, A.
author_sort	Lombardo, M.E.
title	A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde
title_short	A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde
title_full	A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde
title_fullStr	A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde
title_full_unstemmed	A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde
title_sort	method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde
url	http://hdl.handle.net/20.500.12110/paper_09395075_v45_n7-8_p911_Lombardo
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A method for estimating glycine in the presence of excess glutamate with o-phthaldialdehyde

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