Expression of exogenous genes in Trypanosoma cruzi: Improving vectors and electroporation protocols

To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol and expression vectors which use luciferase and green and red fluorescent proteins as reporter genes. In transient transfections, the electroporation conditions reported here resulted in luciferase e...

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Detalles Bibliográficos
Autores principales: DaRocha, W.D., Silva, R.A., Bartholomeu, D.C., Pires, S.F., Freitas, J.M., Macedo, A.M., Vazquez, M.P., Levin, M.J., Teixeira, S.M.R.
Formato: JOUR
Materias:
alpha tubulin
beta tubulin
DNA fragment
immunoglobulin
luciferase
red fluorescent protein
ribosome protein
5' untranslated region
article
electroporation
epimastigote
expression vector
gene expression
microbial genetics
nonhuman
priority journal
reporter gene
Trypanosoma cruzi
5' Untranslated Regions
Animals
Base Sequence
Electroporation
Genes, Reporter
Genetic Vectors
Green Fluorescent Proteins
Luciferases
Luminescent Proteins
Molecular Sequence Data
Protozoan Proteins
Transfection
Tubulin
Protozoa
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_09320113_v92_n2_p113_DaRocha
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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