Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees
The American Foulbrood Disease (AFB) is a fatal larval bee infection. The etiologic agent is the bacterium Paenibacillus larvae. The treatment involves incineration of all contaminated materials, leading to high losses. The Glycerol Monolaurate (GML) is a known antimicrobial potential compound, howe...
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todo:paper_08824010_v97_n_p183_Lopes2023-10-03T15:40:22Z Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees Lopes, L.Q.S. Santos, C.G. de Almeida Vaucher, R. Gende, L. Raffin, R.P. Santos, R.C.V. Antimicrobial activity Beehives Nanoparticle Toxicity antiinfective agent glycerol monolaurate nanocapsule unclassified drug water antiinfective agent lauric acid derivative monoacylglycerol monolaurin American foulbrood animal experiment animal model antimicrobial activity Article bactericidal activity bacteriostasis bee disease controlled release formulation controlled study dilution drug solubility melting point microorganism minimum inhibitory concentration nonhuman Paenibacillus Paenibacillus larvae pharmacological parameters physical chemistry polydispersity index priority journal time kill curve zeta potential animal bacterial spore bee drug effects growth, development and aging microbial sensitivity test microbial viability Paenibacillus larvae physiology survival analysis Animals Anti-Infective Agents Bees Laurates Microbial Sensitivity Tests Microbial Viability Monoglycerides Nanocapsules Paenibacillus larvae Spores, Bacterial Survival Analysis The American Foulbrood Disease (AFB) is a fatal larval bee infection. The etiologic agent is the bacterium Paenibacillus larvae. The treatment involves incineration of all contaminated materials, leading to high losses. The Glycerol Monolaurate (GML) is a known antimicrobial potential compound, however its use is reduced due to its low solubility in water and high melting point. The nanoencapsulation of some drugs offers several advantages like improved stability and solubility in water. The present study aimed to evaluate the antimicrobial activity against P. larvae and the toxicity in bees of GML nanoparticles. The nanocapsules were produced and presented mean diameter of 210 nm, polydispersity index of 0.044, and zeta potential of -23.4 mV demonstrating the acceptable values to predict a stable system. The microdilution assay showed that it is necessary 142 and 285 μg/mL of GML nanocapsules to obtain a bacteriostatic and bactericidal effect respectively. The time-kill curve showed the controlled release of compound, exterminating the microorganism after 24 h. The GML nanocapsules were able to kill the spore form of Paenibacillus larvae while the GML do not cause any effect. The assay in bees showed that the GML has a high toxicity while the GML nanoparticles showed a decrease on toxic effects. Concluding, the formulation shows positive results in the action to combat AFB besides not causing damage to bees. © 2016 Elsevier Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08824010_v97_n_p183_Lopes |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antimicrobial activity Beehives Nanoparticle Toxicity antiinfective agent glycerol monolaurate nanocapsule unclassified drug water antiinfective agent lauric acid derivative monoacylglycerol monolaurin American foulbrood animal experiment animal model antimicrobial activity Article bactericidal activity bacteriostasis bee disease controlled release formulation controlled study dilution drug solubility melting point microorganism minimum inhibitory concentration nonhuman Paenibacillus Paenibacillus larvae pharmacological parameters physical chemistry polydispersity index priority journal time kill curve zeta potential animal bacterial spore bee drug effects growth, development and aging microbial sensitivity test microbial viability Paenibacillus larvae physiology survival analysis Animals Anti-Infective Agents Bees Laurates Microbial Sensitivity Tests Microbial Viability Monoglycerides Nanocapsules Paenibacillus larvae Spores, Bacterial Survival Analysis |
| spellingShingle |
Antimicrobial activity Beehives Nanoparticle Toxicity antiinfective agent glycerol monolaurate nanocapsule unclassified drug water antiinfective agent lauric acid derivative monoacylglycerol monolaurin American foulbrood animal experiment animal model antimicrobial activity Article bactericidal activity bacteriostasis bee disease controlled release formulation controlled study dilution drug solubility melting point microorganism minimum inhibitory concentration nonhuman Paenibacillus Paenibacillus larvae pharmacological parameters physical chemistry polydispersity index priority journal time kill curve zeta potential animal bacterial spore bee drug effects growth, development and aging microbial sensitivity test microbial viability Paenibacillus larvae physiology survival analysis Animals Anti-Infective Agents Bees Laurates Microbial Sensitivity Tests Microbial Viability Monoglycerides Nanocapsules Paenibacillus larvae Spores, Bacterial Survival Analysis Lopes, L.Q.S. Santos, C.G. de Almeida Vaucher, R. Gende, L. Raffin, R.P. Santos, R.C.V. Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees |
| topic_facet |
Antimicrobial activity Beehives Nanoparticle Toxicity antiinfective agent glycerol monolaurate nanocapsule unclassified drug water antiinfective agent lauric acid derivative monoacylglycerol monolaurin American foulbrood animal experiment animal model antimicrobial activity Article bactericidal activity bacteriostasis bee disease controlled release formulation controlled study dilution drug solubility melting point microorganism minimum inhibitory concentration nonhuman Paenibacillus Paenibacillus larvae pharmacological parameters physical chemistry polydispersity index priority journal time kill curve zeta potential animal bacterial spore bee drug effects growth, development and aging microbial sensitivity test microbial viability Paenibacillus larvae physiology survival analysis Animals Anti-Infective Agents Bees Laurates Microbial Sensitivity Tests Microbial Viability Monoglycerides Nanocapsules Paenibacillus larvae Spores, Bacterial Survival Analysis |
| description |
The American Foulbrood Disease (AFB) is a fatal larval bee infection. The etiologic agent is the bacterium Paenibacillus larvae. The treatment involves incineration of all contaminated materials, leading to high losses. The Glycerol Monolaurate (GML) is a known antimicrobial potential compound, however its use is reduced due to its low solubility in water and high melting point. The nanoencapsulation of some drugs offers several advantages like improved stability and solubility in water. The present study aimed to evaluate the antimicrobial activity against P. larvae and the toxicity in bees of GML nanoparticles. The nanocapsules were produced and presented mean diameter of 210 nm, polydispersity index of 0.044, and zeta potential of -23.4 mV demonstrating the acceptable values to predict a stable system. The microdilution assay showed that it is necessary 142 and 285 μg/mL of GML nanocapsules to obtain a bacteriostatic and bactericidal effect respectively. The time-kill curve showed the controlled release of compound, exterminating the microorganism after 24 h. The GML nanocapsules were able to kill the spore form of Paenibacillus larvae while the GML do not cause any effect. The assay in bees showed that the GML has a high toxicity while the GML nanoparticles showed a decrease on toxic effects. Concluding, the formulation shows positive results in the action to combat AFB besides not causing damage to bees. © 2016 Elsevier Ltd. |
| format |
JOUR |
| author |
Lopes, L.Q.S. Santos, C.G. de Almeida Vaucher, R. Gende, L. Raffin, R.P. Santos, R.C.V. |
| author_facet |
Lopes, L.Q.S. Santos, C.G. de Almeida Vaucher, R. Gende, L. Raffin, R.P. Santos, R.C.V. |
| author_sort |
Lopes, L.Q.S. |
| title |
Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees |
| title_short |
Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees |
| title_full |
Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees |
| title_fullStr |
Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees |
| title_full_unstemmed |
Evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against American foulbrood disease agent and toxicity on bees |
| title_sort |
evaluation of antimicrobial activity of glycerol monolaurate nanocapsules against american foulbrood disease agent and toxicity on bees |
| url |
http://hdl.handle.net/20.500.12110/paper_08824010_v97_n_p183_Lopes |
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