Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata

We have studied the developmental expression of the main pupal cuticle glycoprotein, PCG‐100, in the Medfly Ceratitis capitata. A polyclonal antiserum was raised against this protein. Western blotting analysis showed that this glycoprotein is integument‐ and stage‐specific. No PCG‐100 or immunologic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Boccaccio, G.L., Quesada‐Allué, L.A.
Formato:	JOUR
Materias:	Ceratitis capitata insect epidermis integument metamorphosis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07394462_v27_n3_p217_Boccaccio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_07394462_v27_n3_p217_Boccaccio
record_format	dspace
spelling	todo:paper_07394462_v27_n3_p217_Boccaccio2023-10-03T15:38:02Z Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata Boccaccio, G.L. Quesada‐Allué, L.A. Ceratitis capitata insect epidermis integument metamorphosis We have studied the developmental expression of the main pupal cuticle glycoprotein, PCG‐100, in the Medfly Ceratitis capitata. A polyclonal antiserum was raised against this protein. Western blotting analysis showed that this glycoprotein is integument‐ and stage‐specific. No PCG‐100 or immunologically related polypeptides were detected in other tissues or instars. As studied by microinjection of [35S]methionine in individual flies, in vivo synthesis and deposition of PCG‐100 begins approximately 48 h after the onset of pupariation, shortly after the time of head eversion. Synthesis is maximal at 54–64 h, decreases at 72 h, and practically ceases in fully shaped 4‐day‐old pupae. The time required for PCG‐100 deposition into the cuticle was found to be less than 10 min after its synthesis. This is the first time such in vivo analysis has been performed. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc. Fil:Boccaccio, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07394462_v27_n3_p217_Boccaccio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Ceratitis capitata insect epidermis integument metamorphosis
spellingShingle	Ceratitis capitata insect epidermis integument metamorphosis Boccaccio, G.L. Quesada‐Allué, L.A. Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
topic_facet	Ceratitis capitata insect epidermis integument metamorphosis
description	We have studied the developmental expression of the main pupal cuticle glycoprotein, PCG‐100, in the Medfly Ceratitis capitata. A polyclonal antiserum was raised against this protein. Western blotting analysis showed that this glycoprotein is integument‐ and stage‐specific. No PCG‐100 or immunologically related polypeptides were detected in other tissues or instars. As studied by microinjection of [35S]methionine in individual flies, in vivo synthesis and deposition of PCG‐100 begins approximately 48 h after the onset of pupariation, shortly after the time of head eversion. Synthesis is maximal at 54–64 h, decreases at 72 h, and practically ceases in fully shaped 4‐day‐old pupae. The time required for PCG‐100 deposition into the cuticle was found to be less than 10 min after its synthesis. This is the first time such in vivo analysis has been performed. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc.
format	JOUR
author	Boccaccio, G.L. Quesada‐Allué, L.A.
author_facet	Boccaccio, G.L. Quesada‐Allué, L.A.
author_sort	Boccaccio, G.L.
title	Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_short	Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_full	Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_fullStr	Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_full_unstemmed	Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_sort	synthesis and deposition of pcg‐100, the main pupal cuticle glycoprotein of the medfly ceratitis capitata
url	http://hdl.handle.net/20.500.12110/paper_07394462_v27_n3_p217_Boccaccio
work_keys_str_mv	AT boccacciogl synthesisanddepositionofpcg100themainpupalcuticleglycoproteinofthemedflyceratitiscapitata AT quesadaalluela synthesisanddepositionofpcg100themainpupalcuticleglycoproteinofthemedflyceratitiscapitata
_version_	1807319243570544640

Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata

Ejemplares similares