Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells
Mutant cells of the HD33 subline of the Ehrlich‐Lettré ascites tumor synthesize and store glycogen mainly intranuclearly, when growing in vivo, and exclusively in the cytoplasm, when permanently cultivated as a suspension cell strain. To investigate whether there exist differences between glycogen o...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_07302312_v39_n2_p185_Kopun |
| Aporte de: |
| id |
todo:paper_07302312_v39_n2_p185_Kopun |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_07302312_v39_n2_p185_Kopun2023-10-03T15:37:16Z Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells Kopun, M. Granzow, C. Krisman, C.R. carcinoma cell nucleus Ehrlich tumor glycogen glycogen glycogen analysis glycogen isolation neoplasm ultrastructure glycogen animal cell cell culture cell nucleus cytoplasm ehrlich ascites tumor cell glycogen analysis mouse nonhuman ultrastructure Animal Carcinoma, Ehrlich Tumor Cell Nucleus Comparative Study Cytoplasm Glycogen Mice Molecular Weight Mutation Spectrophotometry Support, Non-U.S. Gov't Mutant cells of the HD33 subline of the Ehrlich‐Lettré ascites tumor synthesize and store glycogen mainly intranuclearly, when growing in vivo, and exclusively in the cytoplasm, when permanently cultivated as a suspension cell strain. To investigate whether there exist differences between glycogen of nuclear and cytoplasmic origin, the ultrastructure and the biophysical and biochemical properties of glycogen from in vivo and in vitro grown HD33 ascites cells were compared. Pronounced heterogeneity and differences in glycogen particle ultrastructure were evident in situ and after isolation of the native, high‐molecular polysaccharide. Nuclear glycogen contains a fraction of heavier molecules (up to 2 × 109) and larger particles (up to 340 nm) which could not be found in the cytoplasmic preparations, which contained only particles smaller than 140 nm. The subparticles of β‐type are similar in both nuclear and cytoplasmic glycogen. The absorption spectra and glucose analysis after degradation with phosphorylase and debranching enzyme indicate that nuclear glycogen has a higher degree of branching, associated with a decrease in the average chain length between the branching points, and shorter external polyglucosidic chains than cytoplasmic glycogen. This is the first report about the analysis and properties of isolated nuclear glycogen. Copyright © 1989 Alan R. Liss, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07302312_v39_n2_p185_Kopun |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carcinoma cell nucleus Ehrlich tumor glycogen glycogen glycogen analysis glycogen isolation neoplasm ultrastructure glycogen animal cell cell culture cell nucleus cytoplasm ehrlich ascites tumor cell glycogen analysis mouse nonhuman ultrastructure Animal Carcinoma, Ehrlich Tumor Cell Nucleus Comparative Study Cytoplasm Glycogen Mice Molecular Weight Mutation Spectrophotometry Support, Non-U.S. Gov't |
| spellingShingle |
carcinoma cell nucleus Ehrlich tumor glycogen glycogen glycogen analysis glycogen isolation neoplasm ultrastructure glycogen animal cell cell culture cell nucleus cytoplasm ehrlich ascites tumor cell glycogen analysis mouse nonhuman ultrastructure Animal Carcinoma, Ehrlich Tumor Cell Nucleus Comparative Study Cytoplasm Glycogen Mice Molecular Weight Mutation Spectrophotometry Support, Non-U.S. Gov't Kopun, M. Granzow, C. Krisman, C.R. Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells |
| topic_facet |
carcinoma cell nucleus Ehrlich tumor glycogen glycogen glycogen analysis glycogen isolation neoplasm ultrastructure glycogen animal cell cell culture cell nucleus cytoplasm ehrlich ascites tumor cell glycogen analysis mouse nonhuman ultrastructure Animal Carcinoma, Ehrlich Tumor Cell Nucleus Comparative Study Cytoplasm Glycogen Mice Molecular Weight Mutation Spectrophotometry Support, Non-U.S. Gov't |
| description |
Mutant cells of the HD33 subline of the Ehrlich‐Lettré ascites tumor synthesize and store glycogen mainly intranuclearly, when growing in vivo, and exclusively in the cytoplasm, when permanently cultivated as a suspension cell strain. To investigate whether there exist differences between glycogen of nuclear and cytoplasmic origin, the ultrastructure and the biophysical and biochemical properties of glycogen from in vivo and in vitro grown HD33 ascites cells were compared. Pronounced heterogeneity and differences in glycogen particle ultrastructure were evident in situ and after isolation of the native, high‐molecular polysaccharide. Nuclear glycogen contains a fraction of heavier molecules (up to 2 × 109) and larger particles (up to 340 nm) which could not be found in the cytoplasmic preparations, which contained only particles smaller than 140 nm. The subparticles of β‐type are similar in both nuclear and cytoplasmic glycogen. The absorption spectra and glucose analysis after degradation with phosphorylase and debranching enzyme indicate that nuclear glycogen has a higher degree of branching, associated with a decrease in the average chain length between the branching points, and shorter external polyglucosidic chains than cytoplasmic glycogen. This is the first report about the analysis and properties of isolated nuclear glycogen. Copyright © 1989 Alan R. Liss, Inc. |
| format |
JOUR |
| author |
Kopun, M. Granzow, C. Krisman, C.R. |
| author_facet |
Kopun, M. Granzow, C. Krisman, C.R. |
| author_sort |
Kopun, M. |
| title |
Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells |
| title_short |
Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells |
| title_full |
Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells |
| title_fullStr |
Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells |
| title_full_unstemmed |
Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells |
| title_sort |
comparative study of nuclear and cytoplasmic glycogen isolated from mutant hd33 ascites cells |
| url |
http://hdl.handle.net/20.500.12110/paper_07302312_v39_n2_p185_Kopun |
| work_keys_str_mv |
AT kopunm comparativestudyofnuclearandcytoplasmicglycogenisolatedfrommutanthd33ascitescells AT granzowc comparativestudyofnuclearandcytoplasmicglycogenisolatedfrommutanthd33ascitescells AT krismancr comparativestudyofnuclearandcytoplasmicglycogenisolatedfrommutanthd33ascitescells |
| _version_ |
1807317208620072960 |