Application of BRED technology to construct recombinant D29 reporter phage expressing EGFP

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Bacteriophage Recombineering of Electroporated DNA (BRED) has been described for construction of gene deletion and point mutations in mycobacteriophages. Using BRED, we inserted a Phsp60-egfp cassette (1143 bp) into the mycobacteriophage D29 genome to construct a new reporter phage, which was used f...

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Detalles Bibliográficos
Autores principales: da Silva, J.L., Piuri, M., Broussard, G., J. Marinelli, L., Bastos, G.M., Hirata, R.D.C., Hatfull, G.F., Hirata, M.H.
Formato: JOUR
Materias:
Bacteriophage
Green fluorescent protein
Mycobacterium
Recombineering
DNA
enhanced green fluorescent protein
genomic DNA
analytic method
article
bacteriophage
Bacteriophage Recombineering of Electroporated DNA technology
cytolysis
DNA sequence
gene cassette
gene deletion
gene mutation
nonhuman
point mutation
priority journal
protein expression
Electroporation
Genes, Reporter
Green Fluorescent Proteins
Lysogeny
Mycobacteriophages
Mycobacterium smegmatis
Promoter Regions, Genetic
Sequence Deletion
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03781097_v344_n2_p166_daSilva
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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