Effect of sodium citrate concentration on coagulation assays

The aim of this study was to assess the extent of influence of citrate concentration on the results of routine coagulation assays (prothrombin time (PT) and activated partial thromboplastin time (APTT). PT was evaluated using three tromboplastins of different origin and APTT was determined by four d...

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Autores principales: Duboscq, C., Kordich, L.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03252957_v39_n1_p87_Duboscq
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spelling todo:paper_03252957_v39_n1_p87_Duboscq2023-10-03T15:23:37Z Effect of sodium citrate concentration on coagulation assays Duboscq, C. Kordich, L. Activated partial thromboplastin time Prothrombin time Sodium citrate concentration citrate sodium heparin reagent thromboplastin anticoagulant therapy article blood clotting concentration (parameters) controlled study dose response heparinization human international normalized ratio normal human outpatient partial thromboplastin time prothrombin time sensitivity analysis statistical significance The aim of this study was to assess the extent of influence of citrate concentration on the results of routine coagulation assays (prothrombin time (PT) and activated partial thromboplastin time (APTT). PT was evaluated using three tromboplastins of different origin and APTT was determined by four different APTT reagents. Three methods of clot detecction were used (manual, photo-optic and electromecanic). Three groups were studied: healthy volunteers, outpatients receiving stable dosis of oral anticoagulant therapy and hospitalized patients receiving non fractionated heparin therapy. There were no statistically significant differences of TP expressed in percentaje. In patients receiving dicumarinic therapy, the INR (International Normatized Ratio) obtained with two thromboplastins of lower ISI showed a statistically significant difference between two citrate concentrations. This difference is more important when the RIN is greater than 3.5. Effects of sodium citrate concentration on APTT were satistically significant in all groups when a reagent sensitive to aid the detection of lupus anticoagulant was used. For APTT the greatest change was observed in patients receiving heparin therapy. Fil:Duboscq, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordich, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03252957_v39_n1_p87_Duboscq
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Activated partial thromboplastin time
Prothrombin time
Sodium citrate concentration
citrate sodium
heparin
reagent
thromboplastin
anticoagulant therapy
article
blood clotting
concentration (parameters)
controlled study
dose response
heparinization
human
international normalized ratio
normal human
outpatient
partial thromboplastin time
prothrombin time
sensitivity analysis
statistical significance
spellingShingle Activated partial thromboplastin time
Prothrombin time
Sodium citrate concentration
citrate sodium
heparin
reagent
thromboplastin
anticoagulant therapy
article
blood clotting
concentration (parameters)
controlled study
dose response
heparinization
human
international normalized ratio
normal human
outpatient
partial thromboplastin time
prothrombin time
sensitivity analysis
statistical significance
Duboscq, C.
Kordich, L.
Effect of sodium citrate concentration on coagulation assays
topic_facet Activated partial thromboplastin time
Prothrombin time
Sodium citrate concentration
citrate sodium
heparin
reagent
thromboplastin
anticoagulant therapy
article
blood clotting
concentration (parameters)
controlled study
dose response
heparinization
human
international normalized ratio
normal human
outpatient
partial thromboplastin time
prothrombin time
sensitivity analysis
statistical significance
description The aim of this study was to assess the extent of influence of citrate concentration on the results of routine coagulation assays (prothrombin time (PT) and activated partial thromboplastin time (APTT). PT was evaluated using three tromboplastins of different origin and APTT was determined by four different APTT reagents. Three methods of clot detecction were used (manual, photo-optic and electromecanic). Three groups were studied: healthy volunteers, outpatients receiving stable dosis of oral anticoagulant therapy and hospitalized patients receiving non fractionated heparin therapy. There were no statistically significant differences of TP expressed in percentaje. In patients receiving dicumarinic therapy, the INR (International Normatized Ratio) obtained with two thromboplastins of lower ISI showed a statistically significant difference between two citrate concentrations. This difference is more important when the RIN is greater than 3.5. Effects of sodium citrate concentration on APTT were satistically significant in all groups when a reagent sensitive to aid the detection of lupus anticoagulant was used. For APTT the greatest change was observed in patients receiving heparin therapy.
format JOUR
author Duboscq, C.
Kordich, L.
author_facet Duboscq, C.
Kordich, L.
author_sort Duboscq, C.
title Effect of sodium citrate concentration on coagulation assays
title_short Effect of sodium citrate concentration on coagulation assays
title_full Effect of sodium citrate concentration on coagulation assays
title_fullStr Effect of sodium citrate concentration on coagulation assays
title_full_unstemmed Effect of sodium citrate concentration on coagulation assays
title_sort effect of sodium citrate concentration on coagulation assays
url http://hdl.handle.net/20.500.12110/paper_03252957_v39_n1_p87_Duboscq
work_keys_str_mv AT duboscqc effectofsodiumcitrateconcentrationoncoagulationassays
AT kordichl effectofsodiumcitrateconcentrationoncoagulationassays
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