Active site histidine in pig liver aminolevulic acid dehydratase modified by diethylpyrocarbonate and protected by Zn2+ Ions

1. 1. The effect of diethylpyrocarbonate (DEP) (0.1-0.35 mM) on the purified pig liver aminolevulic acid dehydratase (ALA-D) containing 0.3 g-atoms Zn/subunit, under different pHs (6.0-7.5), temperature (0-18°C) and time (0-60 min) was studied 2. 2. Three histidyl residues/subunit were modified by D...

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Detalles Bibliográficos
Autores principales:	Fukuda, H., Paredes, S.R., del C. Batlle, A.M.
Formato:	JOUR
Materias:	diethyl pyrocarbonate histidine metal porphobilinogen synthase zinc animal article binding site enzymology in vitro study liver metabolism pH swine Animal Binding Sites Diethyl Pyrocarbonate Histidine Hydrogen-Ion Concentration In Vitro Liver Metals Porphobilinogen Synthase Support, Non-U.S. Gov't Swine Zinc
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03050491_v91_n2_p285_Fukuda
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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