Involvement of α-adrenoceptors in norepinephrine-induced prostaglandin E2 release by rat pineal gland in vitro

Rat pineal glands incubated in vitro with 10 or 100 μM norepinephrine (NE) released 51% and 415% more prostaglandin E2 (PGE2) to the medium than in the absence of NE. Phentolamine (10 μM) prevented fully the effect of NE at both concentrations, whereas propranolol failed to affect it significantly....

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Detalles Bibliográficos
Autores principales: Ritta, M.N., Cardinali, D.P.
Formato: JOUR
Materias:
adrenergic supersensitivity
norepinephrine
pineal gland
prostaglandins
superior cervical ganglion
α-adrenoceptors
alpha adrenergic receptor
noradrenalin
phentolamine
propranolol
prostaglandin
prostaglandin e2
animal experiment
endocrine system
in vitro study
pineal body
rat
Adrenergic Fibers
Animal
Denervation
Dinoprostone
Dose-Response Relationship, Drug
Ganglia, Sympathetic
Male
Norepinephrine
Pineal Gland
Prostaglandins E
Radioimmunoassay
Rats
Rats, Inbred Strains
Receptors, Adrenergic
Receptors, Adrenergic, alpha
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03043940_v31_n3_p307_Ritta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Rat pineal glands incubated in vitro with 10 or 100 μM norepinephrine (NE) released 51% and 415% more prostaglandin E2 (PGE2) to the medium than in the absence of NE. Phentolamine (10 μM) prevented fully the effect of NE at both concentrations, whereas propranolol failed to affect it significantly. After superior cervical ganglionectomy (SCGx), NE-induced PGE2 release was significantly higher than in sham-operated controls - an effect also blocked by phentolamine but not by oropranolol. These results suggest that NE releases PGE2 in rat pineals via α-adrenoceptors, acting at a post-synaptic site, and that SCGx induces α-adrenergic supersensitivity in the pinealocytes. © 1982.

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