Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities

Microsomal preparations from malignant human breast tumors catalyzed the transfer of mannose and glucose from GDP-[14C]-Man and UDP-[14C]-Glc into lipid-linked sugars and glycoprotein-like substances. As judged by several criteria the obtained lipid-linked monosaccharides behaved as dolichyl phospha...

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Autores principales: Santa Cruza, A.R., Quesada Allue, L.A., Baldi, A.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03008177_v56_n1_p55_SantaCruza
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spelling todo:paper_03008177_v56_n1_p55_SantaCruza2023-10-03T15:17:52Z Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities Santa Cruza, A.R. Quesada Allue, L.A. Baldi, A. carbon diagnostic agent glucosyltransferase glycoprotein glycosyltransferase guanosine diphosphate mannose isoprenoid phosphate sugar mannosyltransferase nucleoside diphosphate sugar uridine diphosphate glucose uridine diphosphate sugar article biosynthesis breast tumor enzymology female human kinetics metabolism microsome Breast Neoplasms Carbon Radioisotopes Female Glucosyltransferases Glycoproteins Guanosine Diphosphate Mannose Hexosyltransferases Human Kinetics Mannosyltransferases Microsomes Nucleoside Diphosphate Sugars Polyisoprenyl Phosphate Oligosaccharides Support, Non-U.S. Gov't Uridine Diphosphate Glucose Uridine Diphosphate Sugars Microsomal preparations from malignant human breast tumors catalyzed the transfer of mannose and glucose from GDP-[14C]-Man and UDP-[14C]-Glc into lipid-linked sugars and glycoprotein-like substances. As judged by several criteria the obtained lipid-linked monosaccharides behaved as dolichyl phosphate mannose and dolichyl phosphate glucose whereas lipid-linked oligosaccharides behaved as polyprenyl diphosphate derivatives. The optimum conditions for mannosyl and glucosyl - transfer reactions and the effect of dolichyl phosphate, detergent and EDTA on incubation mixture were described. © 1983 Martinus Nijhoff Publishers. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v56_n1_p55_SantaCruza
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic carbon
diagnostic agent
glucosyltransferase
glycoprotein
glycosyltransferase
guanosine diphosphate mannose
isoprenoid phosphate sugar
mannosyltransferase
nucleoside diphosphate sugar
uridine diphosphate glucose
uridine diphosphate sugar
article
biosynthesis
breast tumor
enzymology
female
human
kinetics
metabolism
microsome
Breast Neoplasms
Carbon Radioisotopes
Female
Glucosyltransferases
Glycoproteins
Guanosine Diphosphate Mannose
Hexosyltransferases
Human
Kinetics
Mannosyltransferases
Microsomes
Nucleoside Diphosphate Sugars
Polyisoprenyl Phosphate Oligosaccharides
Support, Non-U.S. Gov't
Uridine Diphosphate Glucose
Uridine Diphosphate Sugars
spellingShingle carbon
diagnostic agent
glucosyltransferase
glycoprotein
glycosyltransferase
guanosine diphosphate mannose
isoprenoid phosphate sugar
mannosyltransferase
nucleoside diphosphate sugar
uridine diphosphate glucose
uridine diphosphate sugar
article
biosynthesis
breast tumor
enzymology
female
human
kinetics
metabolism
microsome
Breast Neoplasms
Carbon Radioisotopes
Female
Glucosyltransferases
Glycoproteins
Guanosine Diphosphate Mannose
Hexosyltransferases
Human
Kinetics
Mannosyltransferases
Microsomes
Nucleoside Diphosphate Sugars
Polyisoprenyl Phosphate Oligosaccharides
Support, Non-U.S. Gov't
Uridine Diphosphate Glucose
Uridine Diphosphate Sugars
Santa Cruza, A.R.
Quesada Allue, L.A.
Baldi, A.
Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities
topic_facet carbon
diagnostic agent
glucosyltransferase
glycoprotein
glycosyltransferase
guanosine diphosphate mannose
isoprenoid phosphate sugar
mannosyltransferase
nucleoside diphosphate sugar
uridine diphosphate glucose
uridine diphosphate sugar
article
biosynthesis
breast tumor
enzymology
female
human
kinetics
metabolism
microsome
Breast Neoplasms
Carbon Radioisotopes
Female
Glucosyltransferases
Glycoproteins
Guanosine Diphosphate Mannose
Hexosyltransferases
Human
Kinetics
Mannosyltransferases
Microsomes
Nucleoside Diphosphate Sugars
Polyisoprenyl Phosphate Oligosaccharides
Support, Non-U.S. Gov't
Uridine Diphosphate Glucose
Uridine Diphosphate Sugars
description Microsomal preparations from malignant human breast tumors catalyzed the transfer of mannose and glucose from GDP-[14C]-Man and UDP-[14C]-Glc into lipid-linked sugars and glycoprotein-like substances. As judged by several criteria the obtained lipid-linked monosaccharides behaved as dolichyl phosphate mannose and dolichyl phosphate glucose whereas lipid-linked oligosaccharides behaved as polyprenyl diphosphate derivatives. The optimum conditions for mannosyl and glucosyl - transfer reactions and the effect of dolichyl phosphate, detergent and EDTA on incubation mixture were described. © 1983 Martinus Nijhoff Publishers.
format JOUR
author Santa Cruza, A.R.
Quesada Allue, L.A.
Baldi, A.
author_facet Santa Cruza, A.R.
Quesada Allue, L.A.
Baldi, A.
author_sort Santa Cruza, A.R.
title Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities
title_short Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities
title_full Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities
title_fullStr Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities
title_full_unstemmed Lipid-bound sugars in malignant human breast tumors - Partial characterization of mannosyl and glucosyl transferase activities
title_sort lipid-bound sugars in malignant human breast tumors - partial characterization of mannosyl and glucosyl transferase activities
url http://hdl.handle.net/20.500.12110/paper_03008177_v56_n1_p55_SantaCruza
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AT quesadaalluela lipidboundsugarsinmalignanthumanbreasttumorspartialcharacterizationofmannosylandglucosyltransferaseactivities
AT baldia lipidboundsugarsinmalignanthumanbreasttumorspartialcharacterizationofmannosylandglucosyltransferaseactivities
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